Real-time PCR detection of Mycoplasma pneumonia in the diagnosis of community-acquired pneumonia

Eddi Di Marco

Research output: Contribution to journalArticle

Abstract

Polymerase chain reaction is a useful technique in microbial diagnostics to detect and quantify DNA or RNA of low abundance. Bacterial and viral nucleic acid can be amplified by PCR upon clinical sample extraction using specific primers for classical qualitative PCR and primers and probes for real-time PCR. Here we describe the Scorpion-probe real-time PCR-based assay that offers thermodynamic advantages due to its kinetic reaction and provides faster performances compared to a classical double-labeled probe-based assays.

Original languageEnglish
Pages (from-to)99-105
Number of pages7
JournalMethods in molecular biology (Clifton, N.J.)
Volume1160
DOIs
Publication statusPublished - 2014

Keywords

  • Community-acquired pneumonia
  • Mycoplasma pneumoniae
  • Quantitative PCR
  • Scorpion Probe

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

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