Abstract
Polymerase chain reaction is a useful technique in microbial diagnostics to detect and quantify DNA or RNA of low abundance. Bacterial and viral nucleic acid can be amplified by PCR upon clinical sample extraction using specific primers for classical qualitative PCR and primers and probes for real-time PCR. Here we describe the Scorpion-probe real-time PCR-based assay that offers thermodynamic advantages due to its kinetic reaction and provides faster performances compared to a classical double-labeled probe-based assays.
Original language | English |
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Pages (from-to) | 99-105 |
Number of pages | 7 |
Journal | Methods in molecular biology (Clifton, N.J.) |
Volume | 1160 |
DOIs | |
Publication status | Published - 2014 |
Keywords
- Community-acquired pneumonia
- Mycoplasma pneumoniae
- Quantitative PCR
- Scorpion Probe
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Medicine(all)