Real Time PCR TaqMan assays for detection of polyomaviruses KIV and WUV in clinical samples

Massimiliano Bergallo, Maria Elena Terlizzi, Sara Astegiano, Marco Ciotti, Muhammed Babakir-Mina, Carlo Federico Perno, Rossana Cavallo, Cristina Costa

Research output: Contribution to journalArticlepeer-review

Abstract

Recently, polyomaviruses KI and WU were identified in the airways of patients with acute respiratory symptoms. The epidemiology and pathogenesis of these two viruses are not fully understood, and the development of molecular assays, such as Real Time PCR, was useful for examining their biology and role in different clinical syndromes. The evaluation of different target regions for the amplification of polyomaviruses KI and WU, comparing published primer/probe sets and sets designed in the laboratory is described and was used for testing 175 clinical specimens (84 stools and 91 tonsils). The results showed that the laboratory designs were more sensitive for the detection of polyomaviruses KI and WU DNA in clinical samples. The choice of the primer/probe set, and primarily of the region for amplification, may be relevant for understanding the pathogenic role of viruses such as polyomaviruses KI and WU.

Original languageEnglish
Pages (from-to)69-74
Number of pages6
JournalJournal of Virological Methods
Volume162
Issue number1-2
DOIs
Publication statusPublished - Jan 2009

Keywords

  • Polyomavirus KI
  • Polyomavirus WU
  • Primer/probe set
  • Real Time polymerase chain reaction

ASJC Scopus subject areas

  • Virology

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