Real-time quantitative assay for routine testing of hcv rna in formalin-fixed, paraffin-embedded liver samples

Elisa Gruppioni, Francesco Vasuri, Michelangelo Fiorentino, Elisa Capizzi, Annalisa Altimari, Maria G. Pirini, Gian L. Grazi, Deborah Malvi, Walter F. Grigioni, Antonia D'Errico-Grigioni

Research output: Contribution to journalArticle

Abstract

The assessment of hepatitis C virus (HCV) RNA in liver tissues is clinically relevant in cases where histology, liver function tests, and HCV serology are not sufficient for a definitive diagnosis of HCV-related hepatitis. We analyzed 215 formalin-fixed, paraffin-embedded liver needle biopsies from patients infected with HCV genotypes 1b and 2. HCV RNA extracted from paraffin sections were quantified by means of a TaqMan real-time reverse transcription-polymerase chain reaction method. The quantification of HCV RNA in liver tissue was correlated with the amount of HCV detected by immunohistochemistry (IHC) on paired frozen biopsies, the HCV RNA load in the serum, and the main serum tests of liver function and cholestasis. HCV RNA was detected by real-time reverse transcription-polymerase chain reaction in 169 liver biopsies (78.6%) with a mean value of 13.59±37.25 IU/ng. Tissue HCV RNA levels strongly correlated with the IHC results (P

Original languageEnglish
Pages (from-to)232-238
Number of pages7
JournalDiagnostic Molecular Pathology
Volume18
Issue number4
DOIs
Publication statusPublished - Dec 2009

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Keywords

  • HCV
  • Immunohistochemistry
  • Liver
  • Real-time RT-PCR

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Molecular Biology

Cite this

Gruppioni, E., Vasuri, F., Fiorentino, M., Capizzi, E., Altimari, A., Pirini, M. G., Grazi, G. L., Malvi, D., Grigioni, W. F., & D'Errico-Grigioni, A. (2009). Real-time quantitative assay for routine testing of hcv rna in formalin-fixed, paraffin-embedded liver samples. Diagnostic Molecular Pathology, 18(4), 232-238. https://doi.org/10.1097/PDM.0b013e3181916e31