Recapitulation of the Roberts syndrome cellular phenotype by inhibition of INCENP, ZWINT-1 and ZW10 genes

Antonio Musio, Tullio Mariani, Cristina Montagna, Desirèe Zambroni, Cesare Ascoli, Thomas Ried, Paolo Vezzoni

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Roberts syndrome is an autosomal recessive disorder characterised primarily by symmetric reduction of all limbs and growth retardation. Patients have been reported to have premature separation of heterochromatin regions of many chromosomes and abnormalities in cell cycle. Given the rarity of the syndrome, the linkage analysis approach is not suitable to identify the responsible gene. In this work, a cell line derived from a patient affected by Roberts syndrome was characterized by cell biology and molecular cytogenetics, including comparative genomic hybridization and spectral karyotype. No recurrent chromosomal rearrangements were identified. Thereafter, based on the fact that premature chromatide separation is a reliable marker of the disease, we used antisense oligonucleotide technologies to inhibit six genes involved in various steps of the correct chromosome segregation, such as chromosome cohesion, kinetochore assembling, spindle checkpoint and spindle formation. We found that the inhibition of INCENP, ZWINT-1, ZW10 genes results in the appearance of mitotic cells characterised by centromere separation, chromosome aneuploidy and micronuclei formation. In addition, INCENP, ZWINT-1, ZW10 antisense-treated chromosome morphology was very similar to that of Roberts chromosome when analysed by atomic force microscopy. We concluded that INCENP, ZWINT-1, ZW10 gene inhibition results in cellular phenocopies of Roberts syndrome. Taken together, these findings support a possible role of these genes in the pathogenesis of Roberts syndrome.

Original languageEnglish
Pages (from-to)33-40
Number of pages8
JournalGene
Volume331
Issue number1-2
DOIs
Publication statusPublished - Apr 28 2004

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Phenotype
Chromosomes
Genes
Kinetochores
Chromosome Segregation
Comparative Genomic Hybridization
Heterochromatin
Centromere
Antisense Oligonucleotides
Atomic Force Microscopy
Aneuploidy
Karyotype
Cytogenetics
Chromosome Aberrations
Cell Biology
Cell Cycle
Extremities
Roberts Syndrome
Technology
Cell Line

Keywords

  • Cell cycle
  • Centromere separation
  • DMEM
  • Dulbeco's minimal essential medium
  • FCS
  • FITC
  • Flourescein isothiocynate
  • Foetal calf serum
  • INCENP
  • PD
  • Population doubling
  • Roberts syndrome
  • SKY
  • TdT-medicated dUTP nick end labelling
  • TUNEL
  • ZW10
  • ZWINT-1

ASJC Scopus subject areas

  • Genetics

Cite this

Recapitulation of the Roberts syndrome cellular phenotype by inhibition of INCENP, ZWINT-1 and ZW10 genes. / Musio, Antonio; Mariani, Tullio; Montagna, Cristina; Zambroni, Desirèe; Ascoli, Cesare; Ried, Thomas; Vezzoni, Paolo.

In: Gene, Vol. 331, No. 1-2, 28.04.2004, p. 33-40.

Research output: Contribution to journalArticle

Musio, Antonio ; Mariani, Tullio ; Montagna, Cristina ; Zambroni, Desirèe ; Ascoli, Cesare ; Ried, Thomas ; Vezzoni, Paolo. / Recapitulation of the Roberts syndrome cellular phenotype by inhibition of INCENP, ZWINT-1 and ZW10 genes. In: Gene. 2004 ; Vol. 331, No. 1-2. pp. 33-40.
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AU - Montagna, Cristina

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AU - Ascoli, Cesare

AU - Ried, Thomas

AU - Vezzoni, Paolo

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AB - Roberts syndrome is an autosomal recessive disorder characterised primarily by symmetric reduction of all limbs and growth retardation. Patients have been reported to have premature separation of heterochromatin regions of many chromosomes and abnormalities in cell cycle. Given the rarity of the syndrome, the linkage analysis approach is not suitable to identify the responsible gene. In this work, a cell line derived from a patient affected by Roberts syndrome was characterized by cell biology and molecular cytogenetics, including comparative genomic hybridization and spectral karyotype. No recurrent chromosomal rearrangements were identified. Thereafter, based on the fact that premature chromatide separation is a reliable marker of the disease, we used antisense oligonucleotide technologies to inhibit six genes involved in various steps of the correct chromosome segregation, such as chromosome cohesion, kinetochore assembling, spindle checkpoint and spindle formation. We found that the inhibition of INCENP, ZWINT-1, ZW10 genes results in the appearance of mitotic cells characterised by centromere separation, chromosome aneuploidy and micronuclei formation. In addition, INCENP, ZWINT-1, ZW10 antisense-treated chromosome morphology was very similar to that of Roberts chromosome when analysed by atomic force microscopy. We concluded that INCENP, ZWINT-1, ZW10 gene inhibition results in cellular phenocopies of Roberts syndrome. Taken together, these findings support a possible role of these genes in the pathogenesis of Roberts syndrome.

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