Recent advances in mass spectrometry analysis of histone post-translational modifications: Potential clinical impact of the PAT-H-MS approach

Tiziana Bonaldi, Roberta Noberini

Research output: Contribution to journalArticlepeer-review

Abstract

Histone post-translational modifications (hPTMs) contribute to the regulation of gene expression and increasing evidence links them to the development of various pathologies, highlighting their potential as biomarkers for prognostic, diagnostic and therapeutic applications. Mass spectrometry (MS) has emerged as a powerful analytical tool for hPTM analysis, which has also been applied to the analysis of epigenetic aberrations in diseases. However, the potential offered by the MS-based hPTM analysis of clinical samples for epigenetic biomarker discovery has been left largely unexploited. This article summarizes the contribution of MS-based approaches to clinical epigenetics, with a special focus on the PAThology tissue analysis of Histones by Mass Spectrometry (PAT-H-MS) approach-which represents the first application of MS-based hPTM analysis to formalin-fixed paraffin-embedded clinical samples-discussing its strengths and limitations, as well as possible implementations.

Original languageEnglish
Pages (from-to)245-250
Number of pages6
JournalExpert Review of Proteomics
Volume13
Issue number3
DOIs
Publication statusPublished - Mar 3 2016

Keywords

  • Biomarker
  • breast cancer
  • epigenetics
  • formalin-fixed paraffin-embedded tissues
  • mass spectrometry
  • PAT-H-MS
  • proteomics
  • translational modification

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

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