Recent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination

Victoria R. Polonis, Bruce K. Brown, Andrew Rosa Borges, Susan Zolla-Pazner, Dimiter S. Dimitrov, Mei Yun Zhang, Susan W. Barnett, Ruth M. Ruprecht, Gabriella Scarlatti, Eva Maria Fenyö, David C. Montefiori, Francine E. McCutchan, Nelson L. Michael

Research output: Contribution to journalArticle

Abstract

In AIDS vaccine development the pendulum has swung towards a renewed emphasis on the potential role for neutralizing antibodies in a successful global vaccine. It is recognized that vaccine-induced antibody performance, as assessed in the available neutralization assays, may well serve as a "gatekeeper" for HIV-1 subunit vaccine prioritization and advancement. As a result, development of a standardized platform for reproducible measurement of neutralizing antibodies has received considerable attention. Here we review current advancements in our knowledge of the performance of different types of antibodies in a traditional primary cell neutralization assay and the newer, more standardized TZM-bl reporter cell line assay. In light of recently revealed differences (see accompanying article) in the results obtained in these two neutralization formats, parallel evaluation with both platforms should be contemplated as an interim solution until a better understanding of immune correlates of protection is achieved.

Original languageEnglish
Pages (from-to)315-320
Number of pages6
JournalVirology
Volume375
Issue number2
DOIs
Publication statusPublished - Jun 5 2008

Keywords

  • Antibodies
  • Human immunodeficiency virus type 1 (HIV-1)
  • Neutralization assays
  • Pseudovirus
  • Standardization
  • Vaccine immune assessment

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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  • Cite this

    Polonis, V. R., Brown, B. K., Borges, A. R., Zolla-Pazner, S., Dimitrov, D. S., Zhang, M. Y., Barnett, S. W., Ruprecht, R. M., Scarlatti, G., Fenyö, E. M., Montefiori, D. C., McCutchan, F. E., & Michael, N. L. (2008). Recent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination. Virology, 375(2), 315-320. https://doi.org/10.1016/j.virol.2008.02.007