Receptor mediated endocytosis of N-acetylglucosamine and mannose exposing molecules by cultured chick embryo hepatocytes.

L. Falasca, A. Lentini, L. Dini

Research output: Contribution to journalArticlepeer-review

Abstract

We studied the receptor mediated endocytosis of a modified glycoprotein (N-acetylglucosamine-BSA) and mannan in cultured hepatocytes isolated from 19-days-old embryos. The binding sites for molecules exposing terminal N-acetylglucosamine (GlcNac) and mannose residues were localized and quantified at the ultrastructural level by means of protein-gold complexes. The binding sites were found to be randomly distributed as single gold particles on cultured hepatocyte cell surfaces not restricted to specialized areas of the plasma membrane. The gold ligands were internalized following a receptor mediated pathway, which was studied at different interval times (15, 30 and 60 min.) after incubating the cells with the electron dense markers.

Original languageEnglish
Pages (from-to)621-627
Number of pages7
JournalCellular and Molecular Biology
Volume38
Issue number5-6
Publication statusPublished - Aug 1992

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Molecular Biology

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