Receptor-operated, but not voltage-operated, calcium channels are involved in basophil leucocyte activation and histamine release

A. Tedeschi, A. Miadonna, M. Lorini, M. Arquati, C. Zanussi

Research output: Contribution to journalArticle

Abstract

Transmembrane calcium flux is a critical step in basophil and mast cell activation and subsequent histamine release. This calcium flux is likely to take place through specialized membrane ion channels. Two types of calcium channels have been described so far: the first type is voltage operated and the second type is receptor operated. Depolarization of cell membrane by K+-rich solutions is followed by voltage-operated channel opening in excitable cells, such as smooth muscle cells. We evaluated whether high K+ extracellular concentrations can trigger basophil activation and histamine release. We found that human basophil leucocytes, showing a normal response to activating signals, such as anti-IgE antiserum and formylmethionine peptide, release no histamine when exposed to K+-rich media, alone or in combination with the K+ carrier valinomycin. These results are consistent with there being receptor-operated, but not voltage-operated, calcium channels in the basophil leucocyte plasma membrane.

Original languageEnglish
Pages (from-to)109-111
Number of pages3
JournalInternational Archives of Allergy and Applied Immunology
Volume90
Issue number1
Publication statusPublished - 1989

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Basophils
Histamine Release
Calcium Channels
Leukocytes
Ion Channels
Cell Membrane
Calcium
Valinomycin
Mast Cells
Smooth Muscle Myocytes
Immune Sera
Peptides

ASJC Scopus subject areas

  • Immunology and Allergy

Cite this

Receptor-operated, but not voltage-operated, calcium channels are involved in basophil leucocyte activation and histamine release. / Tedeschi, A.; Miadonna, A.; Lorini, M.; Arquati, M.; Zanussi, C.

In: International Archives of Allergy and Applied Immunology, Vol. 90, No. 1, 1989, p. 109-111.

Research output: Contribution to journalArticle

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