Receptors for IgM: a feature of subpopulations of both T and B human lymphocytes

S. Romagnani, E. Maggi, R. Biagiotti, G. M. Giudizi, A. Amadori, M. Ricci

Research output: Contribution to journalArticlepeer-review


Receptors for IgM were detected on peripheral blood and tonsil human lymphocytes by a rosette technique with ox red blood cells (ORBC) coated with anti-ORBC rabbit IgM. It was found that the receptors are very sensitive to handling procedures of cells and to low temperatures. An overnight incubation period at 37°C was the optimal condition for the maximum expression of receptors for IgM, but the use of IgM-free media in these cultures was neither an essential nor favourable factor for an optimal rosette formation, when ORBC heavily coated with rabbit IgM were used. The great majority of chronic lymphocytic leukaemia (CLL) patients presented a high number of EA(IgM)-RFC, either on freshly drawn or cultured lymphocytes. By fractionation procedures of normal peripheral blood and tonsil lymphocytes, it was found that a subpopulation of B cells, like T cells, also possess receptors for IgM. The receptors for IgM on B cells are less easily detectable and seem to possess a lower avidity for IgM than those present on T lymphocytes.

Original languageEnglish
Pages (from-to)324-332
Number of pages9
JournalClinical and Experimental Immunology
Issue number2
Publication statusPublished - 1978

ASJC Scopus subject areas

  • Immunology


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