Reciprocal expression of CD70 and of its receptor, CD27, in human long term-activated T and natural killer (NK) cells

Inverse regulation by cytokines and role in induction of cytotoxicity

A. M. Orengo, C. Cantoni, F. Neglia, R. Biassoni, S. Ferrini

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

By transfection of COS cells with an expression vector containing CD70 cDNA we demonstrate that two previously described MoAbs (ED6 and LD6) recognize CD70. By means of these MoAbs, we show that the surface expression of CD70 inversely correlates with the expression of its receptor, CD27, on activated T and NK cell populations and clones, although a subpopulation of cells expressing low density of both molecules exists. In addition, culture in the presence of IL-4 significantly enhances CD27 and reduces CD70 surface expression in phytohaemagglutinin (PHA)-activated peripheral blood lymphocytes (PBL), while tumour necrosis factor-alpha (TNF-α) displays opposite effects, indicating that receptor and ligand are reciprocally regulated by these cytokines. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of CD27 and CD70 mRNA suggests a transcriptional control of CD27 antigen expression in T cell clones. In addition, we show by the use of a re-directed killing assay that in cytotoxic T cell receptor (TCR) α/β+ T cell clones, CD27 molecule may be involved in the regulation of cytolytic functions and may act synergistically with CD2. Finally, CD70 also acts as a signal transducing molecule in some activated CD70+ TCR γ/δ+ T or NK cell clones. In conclusion, our data indicate that D27 and CD70 molecules are differentially expressed and regulated on long terma-ctivated T and NK cells and are involved in the control of cellular functions.

Original languageEnglish
Pages (from-to)608-613
Number of pages6
JournalClinical and Experimental Immunology
Volume107
Issue number3
Publication statusPublished - 1997

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Natural Killer T-Cells
Clone Cells
Cytokines
T-Cell Antigen Receptor
CD27 Antigens
T-Lymphocytes
COS Cells
Phytohemagglutinins
Reverse Transcriptase Polymerase Chain Reaction
Interleukin-4
Transfection
Complementary DNA
Tumor Necrosis Factor-alpha
Lymphocytes
Ligands
Messenger RNA
Population

Keywords

  • Activation
  • CD27
  • CD70
  • NK cells
  • T lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

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title = "Reciprocal expression of CD70 and of its receptor, CD27, in human long term-activated T and natural killer (NK) cells: Inverse regulation by cytokines and role in induction of cytotoxicity",
abstract = "By transfection of COS cells with an expression vector containing CD70 cDNA we demonstrate that two previously described MoAbs (ED6 and LD6) recognize CD70. By means of these MoAbs, we show that the surface expression of CD70 inversely correlates with the expression of its receptor, CD27, on activated T and NK cell populations and clones, although a subpopulation of cells expressing low density of both molecules exists. In addition, culture in the presence of IL-4 significantly enhances CD27 and reduces CD70 surface expression in phytohaemagglutinin (PHA)-activated peripheral blood lymphocytes (PBL), while tumour necrosis factor-alpha (TNF-α) displays opposite effects, indicating that receptor and ligand are reciprocally regulated by these cytokines. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of CD27 and CD70 mRNA suggests a transcriptional control of CD27 antigen expression in T cell clones. In addition, we show by the use of a re-directed killing assay that in cytotoxic T cell receptor (TCR) α/β+ T cell clones, CD27 molecule may be involved in the regulation of cytolytic functions and may act synergistically with CD2. Finally, CD70 also acts as a signal transducing molecule in some activated CD70+ TCR γ/δ+ T or NK cell clones. In conclusion, our data indicate that D27 and CD70 molecules are differentially expressed and regulated on long terma-ctivated T and NK cells and are involved in the control of cellular functions.",
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TY - JOUR

T1 - Reciprocal expression of CD70 and of its receptor, CD27, in human long term-activated T and natural killer (NK) cells

T2 - Inverse regulation by cytokines and role in induction of cytotoxicity

AU - Orengo, A. M.

AU - Cantoni, C.

AU - Neglia, F.

AU - Biassoni, R.

AU - Ferrini, S.

PY - 1997

Y1 - 1997

N2 - By transfection of COS cells with an expression vector containing CD70 cDNA we demonstrate that two previously described MoAbs (ED6 and LD6) recognize CD70. By means of these MoAbs, we show that the surface expression of CD70 inversely correlates with the expression of its receptor, CD27, on activated T and NK cell populations and clones, although a subpopulation of cells expressing low density of both molecules exists. In addition, culture in the presence of IL-4 significantly enhances CD27 and reduces CD70 surface expression in phytohaemagglutinin (PHA)-activated peripheral blood lymphocytes (PBL), while tumour necrosis factor-alpha (TNF-α) displays opposite effects, indicating that receptor and ligand are reciprocally regulated by these cytokines. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of CD27 and CD70 mRNA suggests a transcriptional control of CD27 antigen expression in T cell clones. In addition, we show by the use of a re-directed killing assay that in cytotoxic T cell receptor (TCR) α/β+ T cell clones, CD27 molecule may be involved in the regulation of cytolytic functions and may act synergistically with CD2. Finally, CD70 also acts as a signal transducing molecule in some activated CD70+ TCR γ/δ+ T or NK cell clones. In conclusion, our data indicate that D27 and CD70 molecules are differentially expressed and regulated on long terma-ctivated T and NK cells and are involved in the control of cellular functions.

AB - By transfection of COS cells with an expression vector containing CD70 cDNA we demonstrate that two previously described MoAbs (ED6 and LD6) recognize CD70. By means of these MoAbs, we show that the surface expression of CD70 inversely correlates with the expression of its receptor, CD27, on activated T and NK cell populations and clones, although a subpopulation of cells expressing low density of both molecules exists. In addition, culture in the presence of IL-4 significantly enhances CD27 and reduces CD70 surface expression in phytohaemagglutinin (PHA)-activated peripheral blood lymphocytes (PBL), while tumour necrosis factor-alpha (TNF-α) displays opposite effects, indicating that receptor and ligand are reciprocally regulated by these cytokines. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of CD27 and CD70 mRNA suggests a transcriptional control of CD27 antigen expression in T cell clones. In addition, we show by the use of a re-directed killing assay that in cytotoxic T cell receptor (TCR) α/β+ T cell clones, CD27 molecule may be involved in the regulation of cytolytic functions and may act synergistically with CD2. Finally, CD70 also acts as a signal transducing molecule in some activated CD70+ TCR γ/δ+ T or NK cell clones. In conclusion, our data indicate that D27 and CD70 molecules are differentially expressed and regulated on long terma-ctivated T and NK cells and are involved in the control of cellular functions.

KW - Activation

KW - CD27

KW - CD70

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