Reciprocal hybrid joints demonstrate successive V-J rearrangements on the same chromosome in the human TCR gamma locus

D. Alexandre, P. Chuchana, M. G. Roncarolo, H. Yssel, H. Spits, G. Lefranc, M. P. Lefranc

Research output: Contribution to journalArticlepeer-review

Abstract

Novel variable (V)-joining (J) gene rearrangements are described in the human T cell receptor gamma locus, in which, on the one hand, the V3 variable gene is joined to the heptamer-nonamer recombination signals of the J1 segment and, on the other hand, the J1 segment is joined to the V3 recombination signals through head-to-head fusion. These recombination products, or hybrid joints, have been originated through an inversion of 47 kb DNA. Interestingly the inverted DNA stretch contains a normal V9-JP rearrangement. These findings are the first direct demonstration that successive rearrangements occur, on the same chromosome, in the human T cell receptor gamma locus, and suggest that the chronology of the joining events plays a role in the ontogeny of T cells and their differentiation in γ/δ+ and α/β+ lineages.

Original languageEnglish
Pages (from-to)973-982
Number of pages10
JournalInternational Immunology
Volume3
Issue number10
Publication statusPublished - 1991

Keywords

  • gamma chain
  • inversion
  • recombinase
  • recombination signals
  • T cell receptor
  • variable genes

ASJC Scopus subject areas

  • Immunology

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