Recognition efficiency of the hepatitis B virus polyadenylation signals is tissue specific in transgenic mice

Sabina Perfumo, Laura Amicone, Stefano Colloca, Marco Giorgio, Laura Pozzi, Marco Tripodi

Research output: Contribution to journalArticle

Abstract

The hepatitis B virus genome contains a unique polyadenylation (TATAAA) signal which is differentially utilized in the formation of the various hepatitis B virus transcripts. A head-to-tail multiple-copy insertion of a viral fragment comprising the viral enhancer, the X promoter, the X open reading frame, and the viral poly(A) signal in transgenic mice allowed us to monitor tissue-specific differences in the expression of transcripts initiating from the X promoter. These transcripts are efficiently processed at the first polyadenylation site in the liver, while in the kidney, the brain, and the testis, a portion of the transcripts covers too copies of the transgene, since only the second polyadenylation site is properly recognized. As discussed in this article this observation suggests a tissue-specific distribution of cellular factors involved in polyadenylation.

Original languageEnglish
Pages (from-to)6819-6823
Number of pages5
JournalJournal of Virology
Volume66
Issue number11
Publication statusPublished - Nov 1992

ASJC Scopus subject areas

  • Immunology

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