Abstract
The hepatitis B virus genome contains a unique polyadenylation (TATAAA) signal which is differentially utilized in the formation of the various hepatitis B virus transcripts. A head-to-tail multiple-copy insertion of a viral fragment comprising the viral enhancer, the X promoter, the X open reading frame, and the viral poly(A) signal in transgenic mice allowed us to monitor tissue-specific differences in the expression of transcripts initiating from the X promoter. These transcripts are efficiently processed at the first polyadenylation site in the liver, while in the kidney, the brain, and the testis, a portion of the transcripts covers too copies of the transgene, since only the second polyadenylation site is properly recognized. As discussed in this article this observation suggests a tissue-specific distribution of cellular factors involved in polyadenylation.
| Original language | English |
|---|---|
| Pages (from-to) | 6819-6823 |
| Number of pages | 5 |
| Journal | Journal of Virology |
| Volume | 66 |
| Issue number | 11 |
| Publication status | Published - Nov 1992 |
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ASJC Scopus subject areas
- Immunology
Cite this
Recognition efficiency of the hepatitis B virus polyadenylation signals is tissue specific in transgenic mice. / Perfumo, Sabina; Amicone, Laura; Colloca, Stefano; Giorgio, Marco; Pozzi, Laura; Tripodi, Marco.
In: Journal of Virology, Vol. 66, No. 11, 11.1992, p. 6819-6823.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Recognition efficiency of the hepatitis B virus polyadenylation signals is tissue specific in transgenic mice
AU - Perfumo, Sabina
AU - Amicone, Laura
AU - Colloca, Stefano
AU - Giorgio, Marco
AU - Pozzi, Laura
AU - Tripodi, Marco
PY - 1992/11
Y1 - 1992/11
N2 - The hepatitis B virus genome contains a unique polyadenylation (TATAAA) signal which is differentially utilized in the formation of the various hepatitis B virus transcripts. A head-to-tail multiple-copy insertion of a viral fragment comprising the viral enhancer, the X promoter, the X open reading frame, and the viral poly(A) signal in transgenic mice allowed us to monitor tissue-specific differences in the expression of transcripts initiating from the X promoter. These transcripts are efficiently processed at the first polyadenylation site in the liver, while in the kidney, the brain, and the testis, a portion of the transcripts covers too copies of the transgene, since only the second polyadenylation site is properly recognized. As discussed in this article this observation suggests a tissue-specific distribution of cellular factors involved in polyadenylation.
AB - The hepatitis B virus genome contains a unique polyadenylation (TATAAA) signal which is differentially utilized in the formation of the various hepatitis B virus transcripts. A head-to-tail multiple-copy insertion of a viral fragment comprising the viral enhancer, the X promoter, the X open reading frame, and the viral poly(A) signal in transgenic mice allowed us to monitor tissue-specific differences in the expression of transcripts initiating from the X promoter. These transcripts are efficiently processed at the first polyadenylation site in the liver, while in the kidney, the brain, and the testis, a portion of the transcripts covers too copies of the transgene, since only the second polyadenylation site is properly recognized. As discussed in this article this observation suggests a tissue-specific distribution of cellular factors involved in polyadenylation.
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UR - http://www.scopus.com/inward/citedby.url?scp=0026670507&partnerID=8YFLogxK
M3 - Article
C2 - 1357192
AN - SCOPUS:0026670507
VL - 66
SP - 6819
EP - 6823
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 11
ER -