Recognition imaging and highly ordered molecular templating of bacterial S-layer nanoarrays containing affinity-tags

Jilin Tang, Andreas Ebner, Helga Badelt-Lichtblau, Christine Völlenkle, Christian Rankl, Bernhard Kraxberger, Michael Leitner, Linda Wildling, Hermann J. Gruber, Uwe B. Sleytr, Nicola Ilk, Peter Hinterdorfer

Research output: Contribution to journalArticle

Abstract

Functional nanoarrays were fabricated using the chimeric bacterial cell surface layer (S-layer) protein rSbpA fused with the affinity tag Strep-tagll and characterized using various atomic force microscopy (AFM) techniques in aqueous environment. The accessibility of Strep-tagll was verified by single-molecule force spectroscopy studies employing Strep-Tactin as specific ligand. Simultaneous topography and recognition imaging (TREC) of the nanoarray yielded high resolution maps of the Strep-tagll binding sites with a positional accuracy of 1.5 nm. The nanoarrays were used as template for constructing highly ordered molecular binding blocks.

Original languageEnglish
Pages (from-to)4312-4319
Number of pages8
JournalNano Letters
Volume8
Issue number12
DOIs
Publication statusPublished - Dec 2008

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Mechanical Engineering
  • Materials Science(all)
  • Condensed Matter Physics

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    Tang, J., Ebner, A., Badelt-Lichtblau, H., Völlenkle, C., Rankl, C., Kraxberger, B., Leitner, M., Wildling, L., Gruber, H. J., Sleytr, U. B., Ilk, N., & Hinterdorfer, P. (2008). Recognition imaging and highly ordered molecular templating of bacterial S-layer nanoarrays containing affinity-tags. Nano Letters, 8(12), 4312-4319. https://doi.org/10.1021/nl802092c