Recognition imaging and highly ordered molecular templating of bacterial S-layer nanoarrays containing affinity-tags

Jilin Tang, Andreas Ebner, Helga Badelt-Lichtblau, Christine Völlenkle, Christian Rankl, Bernhard Kraxberger, Michael Leitner, Linda Wildling, Hermann J. Gruber, Uwe B. Sleytr, Nicola Ilk, Peter Hinterdorfer

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Functional nanoarrays were fabricated using the chimeric bacterial cell surface layer (S-layer) protein rSbpA fused with the affinity tag Strep-tagll and characterized using various atomic force microscopy (AFM) techniques in aqueous environment. The accessibility of Strep-tagll was verified by single-molecule force spectroscopy studies employing Strep-Tactin as specific ligand. Simultaneous topography and recognition imaging (TREC) of the nanoarray yielded high resolution maps of the Strep-tagll binding sites with a positional accuracy of 1.5 nm. The nanoarrays were used as template for constructing highly ordered molecular binding blocks.

Original languageEnglish
Pages (from-to)4312-4319
Number of pages8
JournalNano Letters
Volume8
Issue number12
DOIs
Publication statusPublished - Dec 2008

Fingerprint

Binding sites
Topography
affinity
Atomic force microscopy
surface layers
topography
templates
Binding Sites
Ligands
atomic force microscopy
Spectroscopy
proteins
Proteins
Imaging techniques
ligands
Molecules
high resolution
cells
spectroscopy
molecules

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Mechanical Engineering
  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Tang, J., Ebner, A., Badelt-Lichtblau, H., Völlenkle, C., Rankl, C., Kraxberger, B., ... Hinterdorfer, P. (2008). Recognition imaging and highly ordered molecular templating of bacterial S-layer nanoarrays containing affinity-tags. Nano Letters, 8(12), 4312-4319. https://doi.org/10.1021/nl802092c

Recognition imaging and highly ordered molecular templating of bacterial S-layer nanoarrays containing affinity-tags. / Tang, Jilin; Ebner, Andreas; Badelt-Lichtblau, Helga; Völlenkle, Christine; Rankl, Christian; Kraxberger, Bernhard; Leitner, Michael; Wildling, Linda; Gruber, Hermann J.; Sleytr, Uwe B.; Ilk, Nicola; Hinterdorfer, Peter.

In: Nano Letters, Vol. 8, No. 12, 12.2008, p. 4312-4319.

Research output: Contribution to journalArticle

Tang, J, Ebner, A, Badelt-Lichtblau, H, Völlenkle, C, Rankl, C, Kraxberger, B, Leitner, M, Wildling, L, Gruber, HJ, Sleytr, UB, Ilk, N & Hinterdorfer, P 2008, 'Recognition imaging and highly ordered molecular templating of bacterial S-layer nanoarrays containing affinity-tags', Nano Letters, vol. 8, no. 12, pp. 4312-4319. https://doi.org/10.1021/nl802092c
Tang, Jilin ; Ebner, Andreas ; Badelt-Lichtblau, Helga ; Völlenkle, Christine ; Rankl, Christian ; Kraxberger, Bernhard ; Leitner, Michael ; Wildling, Linda ; Gruber, Hermann J. ; Sleytr, Uwe B. ; Ilk, Nicola ; Hinterdorfer, Peter. / Recognition imaging and highly ordered molecular templating of bacterial S-layer nanoarrays containing affinity-tags. In: Nano Letters. 2008 ; Vol. 8, No. 12. pp. 4312-4319.
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