Recombinant gamma-interferon induces in vitro monocytic differentiation of blast cells from patients with acute nonlymphocytic leukemia and myelodysplastic syndromes

C. C. Stella, M. Cazzola, A. Ganser, G. Bergamaschi, F. Meloni, P. Pedrazzoli, P. Bernasconi, R. Invernizzi, D. Hoelzer, E. Ascari

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Abstract

γ-Interferon (IFN-γ) has previously been found to induce monocytic differentiation in established leukemic cell lines, such as HL-60 and U937. The aim of the present sudy was to evaluate the differentiative effect of highly purified recombinant (r)IFN-γ on fresh bone marrow cells from patients with acute nonlymphocytic leukemia (n = 11) or myelodysplastic syndromes (n=3). Blast cells were cultured in suspension in the presence or absence of rIFN-γ (10-103 U/ml). While 6 out of 14 cases were unresponsive to rIFN-γ in vitro, the remaining 8 patients showed a significant increase (0.05 > p > 0.001) in the percentage of cells expressing C3bi receptors, detected by OKM1 (median value in control cell, 9.5; median value in rIFN-γ-treated cells, 31) and Mo1 (8.5 vs. 36), and in the percentage of cells expressing the monocytic antigens detected by Mo2 (8 vs. 28) and MY4 (6.5 vs. 32.5). In the responsive patients morphologic changes consistent with monocytic maturation, as well as a strong increase of α-naphthyl acetate esterase activity and of nitroblue tetrazolium reducing capability were observed upon culture with rIFN-γ. We conclude that (a) rIFN-γ may induce in vitro monocytic differentiation of blasts from acute nonlymphocytic leukemia and myelodysplastic syndrome patients, and that (b) this agent should be investigated for its capacity to be active in vivo.

Original languageEnglish
Pages (from-to)55-59
Number of pages5
JournalLeukemia
Volume2
Issue number1
Publication statusPublished - 1988

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Myelodysplastic Syndromes
Acute Myeloid Leukemia
Interferon-gamma
Cell Differentiation
Complement C3b
Acetylesterase
Nitroblue Tetrazolium
Proxy
Bone Marrow Cells
Interferons
Cultured Cells
Suspensions
Antigens
Cell Line
In Vitro Techniques

ASJC Scopus subject areas

  • Cancer Research
  • Hematology

Cite this

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title = "Recombinant gamma-interferon induces in vitro monocytic differentiation of blast cells from patients with acute nonlymphocytic leukemia and myelodysplastic syndromes",
abstract = "γ-Interferon (IFN-γ) has previously been found to induce monocytic differentiation in established leukemic cell lines, such as HL-60 and U937. The aim of the present sudy was to evaluate the differentiative effect of highly purified recombinant (r)IFN-γ on fresh bone marrow cells from patients with acute nonlymphocytic leukemia (n = 11) or myelodysplastic syndromes (n=3). Blast cells were cultured in suspension in the presence or absence of rIFN-γ (10-103 U/ml). While 6 out of 14 cases were unresponsive to rIFN-γ in vitro, the remaining 8 patients showed a significant increase (0.05 > p > 0.001) in the percentage of cells expressing C3bi receptors, detected by OKM1 (median value in control cell, 9.5; median value in rIFN-γ-treated cells, 31) and Mo1 (8.5 vs. 36), and in the percentage of cells expressing the monocytic antigens detected by Mo2 (8 vs. 28) and MY4 (6.5 vs. 32.5). In the responsive patients morphologic changes consistent with monocytic maturation, as well as a strong increase of α-naphthyl acetate esterase activity and of nitroblue tetrazolium reducing capability were observed upon culture with rIFN-γ. We conclude that (a) rIFN-γ may induce in vitro monocytic differentiation of blasts from acute nonlymphocytic leukemia and myelodysplastic syndrome patients, and that (b) this agent should be investigated for its capacity to be active in vivo.",
author = "Stella, {C. C.} and M. Cazzola and A. Ganser and G. Bergamaschi and F. Meloni and P. Pedrazzoli and P. Bernasconi and R. Invernizzi and D. Hoelzer and E. Ascari",
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T1 - Recombinant gamma-interferon induces in vitro monocytic differentiation of blast cells from patients with acute nonlymphocytic leukemia and myelodysplastic syndromes

AU - Stella, C. C.

AU - Cazzola, M.

AU - Ganser, A.

AU - Bergamaschi, G.

AU - Meloni, F.

AU - Pedrazzoli, P.

AU - Bernasconi, P.

AU - Invernizzi, R.

AU - Hoelzer, D.

AU - Ascari, E.

PY - 1988

Y1 - 1988

N2 - γ-Interferon (IFN-γ) has previously been found to induce monocytic differentiation in established leukemic cell lines, such as HL-60 and U937. The aim of the present sudy was to evaluate the differentiative effect of highly purified recombinant (r)IFN-γ on fresh bone marrow cells from patients with acute nonlymphocytic leukemia (n = 11) or myelodysplastic syndromes (n=3). Blast cells were cultured in suspension in the presence or absence of rIFN-γ (10-103 U/ml). While 6 out of 14 cases were unresponsive to rIFN-γ in vitro, the remaining 8 patients showed a significant increase (0.05 > p > 0.001) in the percentage of cells expressing C3bi receptors, detected by OKM1 (median value in control cell, 9.5; median value in rIFN-γ-treated cells, 31) and Mo1 (8.5 vs. 36), and in the percentage of cells expressing the monocytic antigens detected by Mo2 (8 vs. 28) and MY4 (6.5 vs. 32.5). In the responsive patients morphologic changes consistent with monocytic maturation, as well as a strong increase of α-naphthyl acetate esterase activity and of nitroblue tetrazolium reducing capability were observed upon culture with rIFN-γ. We conclude that (a) rIFN-γ may induce in vitro monocytic differentiation of blasts from acute nonlymphocytic leukemia and myelodysplastic syndrome patients, and that (b) this agent should be investigated for its capacity to be active in vivo.

AB - γ-Interferon (IFN-γ) has previously been found to induce monocytic differentiation in established leukemic cell lines, such as HL-60 and U937. The aim of the present sudy was to evaluate the differentiative effect of highly purified recombinant (r)IFN-γ on fresh bone marrow cells from patients with acute nonlymphocytic leukemia (n = 11) or myelodysplastic syndromes (n=3). Blast cells were cultured in suspension in the presence or absence of rIFN-γ (10-103 U/ml). While 6 out of 14 cases were unresponsive to rIFN-γ in vitro, the remaining 8 patients showed a significant increase (0.05 > p > 0.001) in the percentage of cells expressing C3bi receptors, detected by OKM1 (median value in control cell, 9.5; median value in rIFN-γ-treated cells, 31) and Mo1 (8.5 vs. 36), and in the percentage of cells expressing the monocytic antigens detected by Mo2 (8 vs. 28) and MY4 (6.5 vs. 32.5). In the responsive patients morphologic changes consistent with monocytic maturation, as well as a strong increase of α-naphthyl acetate esterase activity and of nitroblue tetrazolium reducing capability were observed upon culture with rIFN-γ. We conclude that (a) rIFN-γ may induce in vitro monocytic differentiation of blasts from acute nonlymphocytic leukemia and myelodysplastic syndrome patients, and that (b) this agent should be investigated for its capacity to be active in vivo.

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