Recombinant human alpha-2a interferon promotes an atypical process of mast cell secretion with ultrastructural features suggestive for piecemeal degranulation

Enrico Crivellato, Luigi Candussio, Franco Mallardi, Domenico Ribatti

Research output: Contribution to journalArticlepeer-review

Abstract

Purified mast cells (MC), isolated from the rat peritoneum, were stimulated in vitro with recombinant human IFN-α 2a (rhIFN-α 2a) and studied ultrastructurally. Quantitative determination of histamine release was also performed. The following ultrastructural features were observed: (1) dilation of single granules, leading to the formation of cytoplasmic cavities filled with dissolved or eroded matrices; (2) induction of partially empty, non-fused granule containers close to unaltered resting granules, a process very suggestive of piecemeal degranulation; (3) focal exocytosis, characterized by opening of single granules to the cell exterior and/or fusion of a few granules into small secretory channels. Histamine release was slightly increased in rhIFN-α 2a-treated MC, although not to significant levels. These results indicate that rhIFN-α 2a induces a characteristic pattern of degranulation in rat peritoneal MC and that a small proportion of rhIFN-α 2a-stimulated MC shows ultrastructural features suggestive for piecemeal degranulation.

Original languageEnglish
Pages (from-to)507-512
Number of pages6
JournalJournal of Anatomy
Volume201
Issue number6
DOIs
Publication statusPublished - Dec 1 2002

Keywords

  • Atypical degranulation
  • Interferon
  • Mast cell
  • Piecemeal degranulation
  • Rat

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

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