TY - JOUR
T1 - Recombinant human IFN-γ, but not IFN-α or IFN-β, enhances MHC- and non-MHC-encoded glycoproteins by a protein synthesis-dependent mechanism
AU - Giacomini, P.
AU - Tecce, R.
AU - Gambari, R.
AU - Sacchi, A.
AU - Fisher, P. B.
AU - Natali, P. G.
PY - 1988
Y1 - 1988
N2 - Despite quantitative as well as qualitative differences, all three types of IFN (IFN-α, IFN-β, and IFN-γ) modulate the synthesis as well as the expression of class I and class II histocompatibility Ag and a melanoma-associated Ag located in the plasma membrane as well as the cytoplasm of human melanoma cells. By employing inhibitors of RNA and protein synthesis it was demonstrated that IFN-α and -β increase the expression of histocompatibility products and this tumor-associated Ag by a process not requiring new protein synthesis. In contrast, IFN-γ does require de novo protein synthesis for its modulatory activity. Thus, it appears that IFN might trigger various adaptive functions in different cell lineages by inducing at least two separate sets of responses specific for either IFN-α and -β or IFN-γ. Because the induction requirements for (2'-5')-oligoadenylate synthetase as well as for the development of a cellular antiviral state by different IFN also display a similar protein synthesis dependence pattern, the present results suggest that a similar set of cellular mediators may be involved in the modulation of antigenic expression by IFN-γ in human melanoma cells.
AB - Despite quantitative as well as qualitative differences, all three types of IFN (IFN-α, IFN-β, and IFN-γ) modulate the synthesis as well as the expression of class I and class II histocompatibility Ag and a melanoma-associated Ag located in the plasma membrane as well as the cytoplasm of human melanoma cells. By employing inhibitors of RNA and protein synthesis it was demonstrated that IFN-α and -β increase the expression of histocompatibility products and this tumor-associated Ag by a process not requiring new protein synthesis. In contrast, IFN-γ does require de novo protein synthesis for its modulatory activity. Thus, it appears that IFN might trigger various adaptive functions in different cell lineages by inducing at least two separate sets of responses specific for either IFN-α and -β or IFN-γ. Because the induction requirements for (2'-5')-oligoadenylate synthetase as well as for the development of a cellular antiviral state by different IFN also display a similar protein synthesis dependence pattern, the present results suggest that a similar set of cellular mediators may be involved in the modulation of antigenic expression by IFN-γ in human melanoma cells.
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M3 - Article
C2 - 3129509
AN - SCOPUS:0023936064
VL - 140
SP - 3073
EP - 3081
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 9
ER -