Recombinant Mitochondrial Manganese Containing Superoxide Dismutase Protects Against Ochratoxin A-Induced Nephrotoxicity

Roberto Ciarcia, Sara Damiano, Caterina Squillacioti, Nicola Mirabella, Ugo Pagnini, Alessia Florio, Lorella Severino, Giovambattista Capasso, Antonella Borrelli, Aldo Mancini, Silvia Boffo, Gaetano Romano, Antonio Giordano, Salvatore Florio

Research output: Contribution to journalArticle

Abstract

Ochratoxin A (OTA) is a natural mycotoxin, involved in the development of important human and animal diseases. In this work we have studied the role of oxidative stress in the development of OTA nephrotoxicity and the effect of a new recombinant mitochondrial manganese containing superoxide dismutase (rMnSOD) to prevent kidney damage induced by OTA. Blood pressure, glomerular filtration rate and renal histology were analyzed in control rats and in OTA treated rats. In addition, lipid peroxidation, catalase and superoxide dismutase productions were measured. Our data showed that animals treated with OTA presented hypertension and reduction of glomerular filtration rate (GFR). These effects are most probably related to an increase in the reactive oxygen species (ROS) productions. In fact, we have shown that treatment with rMnSOD restored the levels of blood pressure and GFR simultaneously. Moreover, we have noted that OTA induced alteration on glomerular and tubular degeneration and interstitial infiltrates and that use of rMnSOD combined with OTA prevent this renal histological damage confirming the potential therapeutic role in the treatment of rMnSOD OTA nephrotoxicity.

Original languageEnglish
JournalJournal of Cellular Biochemistry
DOIs
Publication statusPublished - 2016

Keywords

  • Nephrotoxicity
  • Ochratoxin A
  • Oxidative stress
  • rMnSOD

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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  • Cite this

    Ciarcia, R., Damiano, S., Squillacioti, C., Mirabella, N., Pagnini, U., Florio, A., Severino, L., Capasso, G., Borrelli, A., Mancini, A., Boffo, S., Romano, G., Giordano, A., & Florio, S. (2016). Recombinant Mitochondrial Manganese Containing Superoxide Dismutase Protects Against Ochratoxin A-Induced Nephrotoxicity. Journal of Cellular Biochemistry. https://doi.org/10.1002/jcb.25425