Recombinant tumor necrosis factor enhances the locomotion of memory and naive B lymphocytes from human tonsils through the selective engagement of the type II receptor

Anna Corcione, Luciano Ottonello, Giuseppe Tortolina, Paola Tasso, Fabio Ghiotto, Irma Airoldi, Giuseppe Taborelli, Fabio Malavasi, Franco Dallegri, Vito Pistoia

Research output: Contribution to journalArticle

Abstract

Recent studies performed in mice knocked out for the tumor necrosis factor (TNF), the lymphotoxin-α, or the type I TNF receptor (R), genes have shown that these animals display gross defects in germinal center (GC) formation, suggesting that members of the TNF and TNFR superfamilies are involved in the control of B-cell migration. Based on these premises, we have here investigated the effects of human recombinant (r) TNF on the polarization and locomotion of tonsillar B cells. rTNF increased the spontaneous polarization and locomotion of unfractionated tonsillar B lymphocytes in a dose-dependent manner by inducing a true chemotactic response. Memory (IgD-, CD38-) and naive (IgD+, CD38-), but not GC (IgD- , CD38+) B cells purified from total tonsillar B lymphocytes, showed a significantly higher locomotion in the presence than in the absence of rTNF. Accordingly, type I and II TNF receptors (TNFRs) were detected by flow cytometry on the surface of memory and naive, but not GC, B lymphocytes. Blocking experiments with monoclonal antibodies to type I or II TNFR showed that rTNF enhanced the spontaneous chemotaxis of memory and naive B cells through the selective engagement of type II TNFR. Finally, the TNF gene was found to be expressed in memory, naive and GC B lymphocytes; the cytokine was released in culture supernatants from the three B-cell subsets after stimulation. These data may support the hypothesis that human TNF is involved in the paracrine and perhaps autocrine control of B-cell migration in secondary lymphoid tissues.

Original languageEnglish
Pages (from-to)4493-4501
Number of pages9
JournalBlood
Volume90
Issue number11
Publication statusPublished - Dec 1 1997

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Lymphocytes
Palatine Tonsil
Locomotion
B-Lymphocytes
Tumor Necrosis Factor-alpha
Tumor Necrosis Factor Receptors
Cells
Data storage equipment
Immunoglobulin D
Germinal Center
Receptors, Tumor Necrosis Factor, Type I
Genes
Receptors, Tumor Necrosis Factor, Type II
Polarization
Lymphotoxin-alpha
Cell Movement
Flow cytometry
Cell culture
vpr Genes
B-Lymphocyte Subsets

ASJC Scopus subject areas

  • Hematology

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Recombinant tumor necrosis factor enhances the locomotion of memory and naive B lymphocytes from human tonsils through the selective engagement of the type II receptor. / Corcione, Anna; Ottonello, Luciano; Tortolina, Giuseppe; Tasso, Paola; Ghiotto, Fabio; Airoldi, Irma; Taborelli, Giuseppe; Malavasi, Fabio; Dallegri, Franco; Pistoia, Vito.

In: Blood, Vol. 90, No. 11, 01.12.1997, p. 4493-4501.

Research output: Contribution to journalArticle

Corcione, A, Ottonello, L, Tortolina, G, Tasso, P, Ghiotto, F, Airoldi, I, Taborelli, G, Malavasi, F, Dallegri, F & Pistoia, V 1997, 'Recombinant tumor necrosis factor enhances the locomotion of memory and naive B lymphocytes from human tonsils through the selective engagement of the type II receptor', Blood, vol. 90, no. 11, pp. 4493-4501.
Corcione, Anna ; Ottonello, Luciano ; Tortolina, Giuseppe ; Tasso, Paola ; Ghiotto, Fabio ; Airoldi, Irma ; Taborelli, Giuseppe ; Malavasi, Fabio ; Dallegri, Franco ; Pistoia, Vito. / Recombinant tumor necrosis factor enhances the locomotion of memory and naive B lymphocytes from human tonsils through the selective engagement of the type II receptor. In: Blood. 1997 ; Vol. 90, No. 11. pp. 4493-4501.
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