Recovery from 6-month spaceflight at the International Space Station: muscle-related stress into a proinflammatory setting

Miriam Capri, Cristina Morsiani, Aurelia Santoro, Manuela Moriggi, Maria Conte, Morena Martucci, Elena Bellavista, Cristina Fabbri, Enrico Giampieri, Kirsten Albracht, Martin Flück, Severin Ruoss, Lorenza Brocca, Monica Canepari, Emanuela Longa, Irene Di Giulio, Roberto Bottinelli, Paolo Cerretelli, Stefano Salvioli, Cecilia GelfiClaudio Franceschi, Marco Narici, Jörn Rittweger

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The Sarcolab pilot study of 2 crewmembers, investigated before and after a 6-mo International Space Station mission, has demonstrated the substantial muscle wasting and weakness, along with disruption of muscle's oxidative metabolism. The present work aimed at evaluating the pro/anti-inflammatory status in the same 2 crewmembers (A, B). Blood circulating (c-)microRNAs (miRs), c-proteasome, c-mitochondrial DNA, and cytokines were assessed by real-time quantitative PCR or ELISA tests. Time series analysis was performed ( i.e., before flight and after landing) at 1 and 15 d of recovery (R+1 and R+15, respectively). C-biomarkers were compared with an age-matched control population and with 2-dimensional proteomic analysis of the 2 crewmembers' muscle biopsies. Striking differences were observed between the 2 crewmembers at R+1, in terms of inflamma-miRs (c-miRs-21-5p, -126-3p, and -146a-5p), muscle specific (myo)-miR-206, c-proteasome, and IL-6/leptin, thus making the 2 astronauts dissimilar to each other. Final recovery levels of c-proteasome, c-inflamma-miRs, and c-myo-miR-206 were not reverted to the baseline values in crewmember A. In both crewmembers, myo-miR-206 changed significantly after recovery. Muscle biopsy of astronaut A showed an impressive 80% increase of α-1-antitrypsin, a target of miR-126-3p. These results point to a strong stress response induced by spaceflight involving muscle tissue and the proinflammatory setting, where inflamma-miRs and myo-miR-206 mediate the systemic recovery phase after landing.-Capri, M., Morsiani, C., Santoro, A., Moriggi, M., Conte, M., Martucci, M., Bellavista, E., Fabbri, C., Giampieri, E., Albracht, K., Flück, M., Ruoss, S., Brocca, L., Canepari, M., Longa, E., Di Giulio, I., Bottinelli, R., Cerretelli, P., Salvioli, S., Gelfi, C., Franceschi, C., Narici, M., Rittweger, J. Recovery from 6-month spaceflight at the International Space Station: muscle-related stress into a proinflammatory setting.

Original languageEnglish
Pages (from-to)5168-5180
Number of pages13
JournalFASEB journal : official publication of the Federation of American Societies for Experimental Biology
Volume33
Issue number4
DOIs
Publication statusPublished - Apr 1 2019

Fingerprint

Space Flight
Space stations
Muscle
MicroRNAs
Recovery
Muscles
Proteasome Endopeptidase Complex
Astronauts
Biopsy
Landing
Muscle Weakness
Leptin
Time series analysis
Mitochondrial DNA
Proteomics
Biomarkers
Real-Time Polymerase Chain Reaction
Interleukin-6
Metabolism
Anti-Inflammatory Agents

Keywords

  • inflamma-miRs
  • microRNA-206
  • proteasome
  • SERPINA1

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Recovery from 6-month spaceflight at the International Space Station : muscle-related stress into a proinflammatory setting. / Capri, Miriam; Morsiani, Cristina; Santoro, Aurelia; Moriggi, Manuela; Conte, Maria; Martucci, Morena; Bellavista, Elena; Fabbri, Cristina; Giampieri, Enrico; Albracht, Kirsten; Flück, Martin; Ruoss, Severin; Brocca, Lorenza; Canepari, Monica; Longa, Emanuela; Di Giulio, Irene; Bottinelli, Roberto; Cerretelli, Paolo; Salvioli, Stefano; Gelfi, Cecilia; Franceschi, Claudio; Narici, Marco; Rittweger, Jörn.

In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 33, No. 4, 01.04.2019, p. 5168-5180.

Research output: Contribution to journalArticle

Capri, M, Morsiani, C, Santoro, A, Moriggi, M, Conte, M, Martucci, M, Bellavista, E, Fabbri, C, Giampieri, E, Albracht, K, Flück, M, Ruoss, S, Brocca, L, Canepari, M, Longa, E, Di Giulio, I, Bottinelli, R, Cerretelli, P, Salvioli, S, Gelfi, C, Franceschi, C, Narici, M & Rittweger, J 2019, 'Recovery from 6-month spaceflight at the International Space Station: muscle-related stress into a proinflammatory setting', FASEB journal : official publication of the Federation of American Societies for Experimental Biology, vol. 33, no. 4, pp. 5168-5180. https://doi.org/10.1096/fj.201801625R
Capri, Miriam ; Morsiani, Cristina ; Santoro, Aurelia ; Moriggi, Manuela ; Conte, Maria ; Martucci, Morena ; Bellavista, Elena ; Fabbri, Cristina ; Giampieri, Enrico ; Albracht, Kirsten ; Flück, Martin ; Ruoss, Severin ; Brocca, Lorenza ; Canepari, Monica ; Longa, Emanuela ; Di Giulio, Irene ; Bottinelli, Roberto ; Cerretelli, Paolo ; Salvioli, Stefano ; Gelfi, Cecilia ; Franceschi, Claudio ; Narici, Marco ; Rittweger, Jörn. / Recovery from 6-month spaceflight at the International Space Station : muscle-related stress into a proinflammatory setting. In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2019 ; Vol. 33, No. 4. pp. 5168-5180.
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AU - Capri, Miriam

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AU - Santoro, Aurelia

AU - Moriggi, Manuela

AU - Conte, Maria

AU - Martucci, Morena

AU - Bellavista, Elena

AU - Fabbri, Cristina

AU - Giampieri, Enrico

AU - Albracht, Kirsten

AU - Flück, Martin

AU - Ruoss, Severin

AU - Brocca, Lorenza

AU - Canepari, Monica

AU - Longa, Emanuela

AU - Di Giulio, Irene

AU - Bottinelli, Roberto

AU - Cerretelli, Paolo

AU - Salvioli, Stefano

AU - Gelfi, Cecilia

AU - Franceschi, Claudio

AU - Narici, Marco

AU - Rittweger, Jörn

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N2 - The Sarcolab pilot study of 2 crewmembers, investigated before and after a 6-mo International Space Station mission, has demonstrated the substantial muscle wasting and weakness, along with disruption of muscle's oxidative metabolism. The present work aimed at evaluating the pro/anti-inflammatory status in the same 2 crewmembers (A, B). Blood circulating (c-)microRNAs (miRs), c-proteasome, c-mitochondrial DNA, and cytokines were assessed by real-time quantitative PCR or ELISA tests. Time series analysis was performed ( i.e., before flight and after landing) at 1 and 15 d of recovery (R+1 and R+15, respectively). C-biomarkers were compared with an age-matched control population and with 2-dimensional proteomic analysis of the 2 crewmembers' muscle biopsies. Striking differences were observed between the 2 crewmembers at R+1, in terms of inflamma-miRs (c-miRs-21-5p, -126-3p, and -146a-5p), muscle specific (myo)-miR-206, c-proteasome, and IL-6/leptin, thus making the 2 astronauts dissimilar to each other. Final recovery levels of c-proteasome, c-inflamma-miRs, and c-myo-miR-206 were not reverted to the baseline values in crewmember A. In both crewmembers, myo-miR-206 changed significantly after recovery. Muscle biopsy of astronaut A showed an impressive 80% increase of α-1-antitrypsin, a target of miR-126-3p. These results point to a strong stress response induced by spaceflight involving muscle tissue and the proinflammatory setting, where inflamma-miRs and myo-miR-206 mediate the systemic recovery phase after landing.-Capri, M., Morsiani, C., Santoro, A., Moriggi, M., Conte, M., Martucci, M., Bellavista, E., Fabbri, C., Giampieri, E., Albracht, K., Flück, M., Ruoss, S., Brocca, L., Canepari, M., Longa, E., Di Giulio, I., Bottinelli, R., Cerretelli, P., Salvioli, S., Gelfi, C., Franceschi, C., Narici, M., Rittweger, J. Recovery from 6-month spaceflight at the International Space Station: muscle-related stress into a proinflammatory setting.

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