Recovery from mild Escherichia coli O157:H7 infection in young and aged C57BL/6 mice with intact flora estimated by fecal shedding, locomotor activity and grip strength

Marco Malavolta, Andrea Basso, Robertina Giacconi, Fiorenza Orlando, Elisa Pierpaoli, Maurizio Cardelli, Francesca Leoni, Serena Chierichetti, Dorothy Bray, Khadija Benlhassan, Mauro Provinciali

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Escherichia coli 0157:H7 is a food-borne pathogen that can cause severe complications in vulnerable populations. Mouse infection models of E. coli 0157:H7 are usually developed under severe animal suffering classification by depleting the normal flora, in which age plays a role. Objective: To develop a refined method for longitudinal monitoring of E. coli 0157:H7 in young and old mice with intact flora. Methods: We applied discriminant analysis and computed composite standardized scores from 19 variables obtained from physiological parameters, analysis of locomotor activity, grip strength measurement and fecal shedding in 16 aged and 16 young C57BL/6 mice after two mild oral challenges of E. coli 0157:H7. The resulting scores were validated in another experiment performed in 24 aged and 24 young mice including a group (8 aged and 8 young mice) treated with oxytetracycline. Results: We show that our scores are significantly affected in the post-infection period and that can be used to measure and compare the recovery time after a treatment. The scores are most sensitive when separately developed in young and aged mice. Conclusions: We developed a method that minimizes the level of animal suffering and that can be applied in preclinical testing of new therapies.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume63
DOIs
Publication statusPublished - Apr 1 2019

Fingerprint

Escherichia coli O157
Hand Strength
Locomotion
Inbred C57BL Mouse
locomotion
flora
mice
Infection
infection
Escherichia coli
Escherichia coli Infections
Oxytetracycline
Discriminant Analysis
Vulnerable Populations
Psychological Stress
oxytetracycline
food pathogens
discriminant analysis
mouth
animals

Keywords

  • Aging
  • Escherichia coli
  • Grip strength
  • Locomotor activity
  • Non-invasive phenotyping

ASJC Scopus subject areas

  • Microbiology
  • Immunology and Allergy
  • Immunology
  • veterinary(all)
  • Infectious Diseases

Cite this

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title = "Recovery from mild Escherichia coli O157:H7 infection in young and aged C57BL/6 mice with intact flora estimated by fecal shedding, locomotor activity and grip strength",
abstract = "Escherichia coli 0157:H7 is a food-borne pathogen that can cause severe complications in vulnerable populations. Mouse infection models of E. coli 0157:H7 are usually developed under severe animal suffering classification by depleting the normal flora, in which age plays a role. Objective: To develop a refined method for longitudinal monitoring of E. coli 0157:H7 in young and old mice with intact flora. Methods: We applied discriminant analysis and computed composite standardized scores from 19 variables obtained from physiological parameters, analysis of locomotor activity, grip strength measurement and fecal shedding in 16 aged and 16 young C57BL/6 mice after two mild oral challenges of E. coli 0157:H7. The resulting scores were validated in another experiment performed in 24 aged and 24 young mice including a group (8 aged and 8 young mice) treated with oxytetracycline. Results: We show that our scores are significantly affected in the post-infection period and that can be used to measure and compare the recovery time after a treatment. The scores are most sensitive when separately developed in young and aged mice. Conclusions: We developed a method that minimizes the level of animal suffering and that can be applied in preclinical testing of new therapies.",
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author = "Marco Malavolta and Andrea Basso and Robertina Giacconi and Fiorenza Orlando and Elisa Pierpaoli and Maurizio Cardelli and Francesca Leoni and Serena Chierichetti and Dorothy Bray and Khadija Benlhassan and Mauro Provinciali",
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T1 - Recovery from mild Escherichia coli O157:H7 infection in young and aged C57BL/6 mice with intact flora estimated by fecal shedding, locomotor activity and grip strength

AU - Malavolta, Marco

AU - Basso, Andrea

AU - Giacconi, Robertina

AU - Orlando, Fiorenza

AU - Pierpaoli, Elisa

AU - Cardelli, Maurizio

AU - Leoni, Francesca

AU - Chierichetti, Serena

AU - Bray, Dorothy

AU - Benlhassan, Khadija

AU - Provinciali, Mauro

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Escherichia coli 0157:H7 is a food-borne pathogen that can cause severe complications in vulnerable populations. Mouse infection models of E. coli 0157:H7 are usually developed under severe animal suffering classification by depleting the normal flora, in which age plays a role. Objective: To develop a refined method for longitudinal monitoring of E. coli 0157:H7 in young and old mice with intact flora. Methods: We applied discriminant analysis and computed composite standardized scores from 19 variables obtained from physiological parameters, analysis of locomotor activity, grip strength measurement and fecal shedding in 16 aged and 16 young C57BL/6 mice after two mild oral challenges of E. coli 0157:H7. The resulting scores were validated in another experiment performed in 24 aged and 24 young mice including a group (8 aged and 8 young mice) treated with oxytetracycline. Results: We show that our scores are significantly affected in the post-infection period and that can be used to measure and compare the recovery time after a treatment. The scores are most sensitive when separately developed in young and aged mice. Conclusions: We developed a method that minimizes the level of animal suffering and that can be applied in preclinical testing of new therapies.

AB - Escherichia coli 0157:H7 is a food-borne pathogen that can cause severe complications in vulnerable populations. Mouse infection models of E. coli 0157:H7 are usually developed under severe animal suffering classification by depleting the normal flora, in which age plays a role. Objective: To develop a refined method for longitudinal monitoring of E. coli 0157:H7 in young and old mice with intact flora. Methods: We applied discriminant analysis and computed composite standardized scores from 19 variables obtained from physiological parameters, analysis of locomotor activity, grip strength measurement and fecal shedding in 16 aged and 16 young C57BL/6 mice after two mild oral challenges of E. coli 0157:H7. The resulting scores were validated in another experiment performed in 24 aged and 24 young mice including a group (8 aged and 8 young mice) treated with oxytetracycline. Results: We show that our scores are significantly affected in the post-infection period and that can be used to measure and compare the recovery time after a treatment. The scores are most sensitive when separately developed in young and aged mice. Conclusions: We developed a method that minimizes the level of animal suffering and that can be applied in preclinical testing of new therapies.

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