Recovery of human lymphocytes damaged with γradiation or enzymatically produced oxygen radicals: Different effects of poly(ADP-ribosyl)polymerase inhibitors

M. Marini, G. Zunica, M. Tamba, A. Cossarizza, D. Monti, C. Franceschi

Research output: Contribution to journalArticle

Abstract

Quiescent human lymphocytes were damaged in two different ways, both producing toxic oxygen radicals: xanthine oxidase plus hypoxanthine (XOD/HYP), or γrays. Under conditions where DNA synthesis was reduced to 10-20% of control, inhibitors of poly(ADP-ribosyl)polymerase (ADPRP, an enzyme that becomes activated in the presence of DNA strand breaks) allowed lymphocytes to recover completely when the damage was caused by XOD/HYP, but they did not affect DNA synthesis of irradiated cells. However, a protective effect of ADPRP inhibitors was observed with irradiated lymphocytes receiving doses ≥ 50 Gy. Unscheduled DNA synthesis was significantly increased when lymphocytes were damaged by high radiation doses in the presence of ADPRP inhibitors. We suggest that ionizing radiation does not stimulate poly(ADP-ribose) synthesis in lymphocytes at doses that impair lymphocyte DNA synthesis by 80-90%, while ADPRP may be involved in the repair of DNA lesions occurring at higher radiation doses.

Original languageEnglish
Pages (from-to)279-291
Number of pages13
JournalInternational Journal of Radiation Biology
Volume58
Issue number2
DOIs
Publication statusPublished - 1990

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Agricultural and Biological Sciences (miscellaneous)
  • Nuclear Energy and Engineering
  • Radiation

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