Recurrent pregnancy loss is associated to leaky gut

A novel pathogenic model of endometrium inflammation?

C. Tersigni, S. D'Ippolito, F. Di Nicuolo, R. Marana, V. Valenza, V. Masciullo, F. Scaldaferri, F. Malatacca, C. Waure, A. Gasbarrini, G. Scambia, N. Di Simone

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Recurrent pregnancy loss (RPL) occurs in 3-5% in about 30% of cases no cause can be found. Women with RPL show higher prevalence of undiagnosed gut disorders. Furthermore, in endometrial tissues of RPL women, higher expression of pro-inflammatory cytokines and Nalp-3 inflammasome has been observed. Aim of this study was to investigate whether an abnormal gut permeability might occur in RPL women and allow passage into systemic circulation of pro-inflammatory molecules able to induce endometrial inflammation. Methods: 70 women with idiopathic RPL and 30 healthy women were recruited at the Recurrent Pregnancy Loss Outpatient Unit of the Gemelli Hospital of Rome from March 2013 to February 2017. Enrolled women underwent 51Cr-ethylene-diamine-tetraacetic acid absorption test to evaluate intestinal permeability. Sera obtained from enrolled women were analysed for lipopolysaccharide (LPS) by ELISA. Anxiety and depression state were evaluated by administering STAI-Y and Zung-SDS tests, respectively. Of all recruited individuals, 35 women with idiopathic RPL and 20 healthy controls accepted to undergo diagnostic hysteroscopy and endometrial biopsy. Endometrial lysates were investigated for inflammasome Nalp-3 by Western blot analysis, and caspase-1, IL-1β and IL-18 by ELISA, respectively. Results: Higher prevalence of abnormal intestinal permeability (P<0.0001), increased circulating levels of LPS (P<0.05), anxiety (P<0.05) and depression (P<0.05) were observed in RLP women compared to controls. Endometrial expression of Nalp-3, caspase-1 and IL-1β was significantly increased in RPL group (P<0.0001; P<0.05 and P<0.001, respectively). IL-18 endometrial levels were not found to be higher in RPL cases. Statistically significant association between higher intestinal permeability and abnormally increased expression of endometrial Nalp-3, was observed in RPL (P<0.01). Furthermore, higher LPS serum levels, a bacterial-derived activator of Nalp-3 complex, was shown to be statistically associated to abnormal endometrial expression of Nalp-3 inflammasome (P<0.01) in RPL women. Conclusions: In women with RLP, leaky gut might occur and allow passage into circulation of immune triggers, potentially able to elicit endometrial innate immune response and, thus, to contribute to miscarriage pathogenesis. Diagnosis and treatment of intestinal disorders underlying leaky gut might improve endometrial environment and pregnancy outcome.

Original languageEnglish
Article number102
JournalJournal of Translational Medicine
Volume16
Issue number1
DOIs
Publication statusPublished - Apr 17 2018

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Endometrium
Inflammation
Pregnancy
Inflammasomes
Permeability
Lipopolysaccharides
Caspase 1
Interleukin-18
Interleukin-1
Anxiety
Enzyme-Linked Immunosorbent Assay
Depression
Hysteroscopy
Hospital Units
Diamines
Biopsy
Spontaneous Abortion
Pregnancy Outcome
Serum
Innate Immunity

Keywords

  • Human endometrium
  • Inflammasome
  • Intestinal permeability
  • Recurrent pregnancy loss

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Tersigni, C., D'Ippolito, S., Di Nicuolo, F., Marana, R., Valenza, V., Masciullo, V., ... Di Simone, N. (2018). Recurrent pregnancy loss is associated to leaky gut: A novel pathogenic model of endometrium inflammation? Journal of Translational Medicine, 16(1), [102]. https://doi.org/10.1186/s12967-018-1482-y

Recurrent pregnancy loss is associated to leaky gut : A novel pathogenic model of endometrium inflammation? / Tersigni, C.; D'Ippolito, S.; Di Nicuolo, F.; Marana, R.; Valenza, V.; Masciullo, V.; Scaldaferri, F.; Malatacca, F.; Waure, C.; Gasbarrini, A.; Scambia, G.; Di Simone, N.

In: Journal of Translational Medicine, Vol. 16, No. 1, 102, 17.04.2018.

Research output: Contribution to journalArticle

Tersigni, C, D'Ippolito, S, Di Nicuolo, F, Marana, R, Valenza, V, Masciullo, V, Scaldaferri, F, Malatacca, F, Waure, C, Gasbarrini, A, Scambia, G & Di Simone, N 2018, 'Recurrent pregnancy loss is associated to leaky gut: A novel pathogenic model of endometrium inflammation?', Journal of Translational Medicine, vol. 16, no. 1, 102. https://doi.org/10.1186/s12967-018-1482-y
Tersigni, C. ; D'Ippolito, S. ; Di Nicuolo, F. ; Marana, R. ; Valenza, V. ; Masciullo, V. ; Scaldaferri, F. ; Malatacca, F. ; Waure, C. ; Gasbarrini, A. ; Scambia, G. ; Di Simone, N. / Recurrent pregnancy loss is associated to leaky gut : A novel pathogenic model of endometrium inflammation?. In: Journal of Translational Medicine. 2018 ; Vol. 16, No. 1.
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abstract = "Background: Recurrent pregnancy loss (RPL) occurs in 3-5{\%} in about 30{\%} of cases no cause can be found. Women with RPL show higher prevalence of undiagnosed gut disorders. Furthermore, in endometrial tissues of RPL women, higher expression of pro-inflammatory cytokines and Nalp-3 inflammasome has been observed. Aim of this study was to investigate whether an abnormal gut permeability might occur in RPL women and allow passage into systemic circulation of pro-inflammatory molecules able to induce endometrial inflammation. Methods: 70 women with idiopathic RPL and 30 healthy women were recruited at the Recurrent Pregnancy Loss Outpatient Unit of the Gemelli Hospital of Rome from March 2013 to February 2017. Enrolled women underwent 51Cr-ethylene-diamine-tetraacetic acid absorption test to evaluate intestinal permeability. Sera obtained from enrolled women were analysed for lipopolysaccharide (LPS) by ELISA. Anxiety and depression state were evaluated by administering STAI-Y and Zung-SDS tests, respectively. Of all recruited individuals, 35 women with idiopathic RPL and 20 healthy controls accepted to undergo diagnostic hysteroscopy and endometrial biopsy. Endometrial lysates were investigated for inflammasome Nalp-3 by Western blot analysis, and caspase-1, IL-1β and IL-18 by ELISA, respectively. Results: Higher prevalence of abnormal intestinal permeability (P<0.0001), increased circulating levels of LPS (P<0.05), anxiety (P<0.05) and depression (P<0.05) were observed in RLP women compared to controls. Endometrial expression of Nalp-3, caspase-1 and IL-1β was significantly increased in RPL group (P<0.0001; P<0.05 and P<0.001, respectively). IL-18 endometrial levels were not found to be higher in RPL cases. Statistically significant association between higher intestinal permeability and abnormally increased expression of endometrial Nalp-3, was observed in RPL (P<0.01). Furthermore, higher LPS serum levels, a bacterial-derived activator of Nalp-3 complex, was shown to be statistically associated to abnormal endometrial expression of Nalp-3 inflammasome (P<0.01) in RPL women. Conclusions: In women with RLP, leaky gut might occur and allow passage into circulation of immune triggers, potentially able to elicit endometrial innate immune response and, thus, to contribute to miscarriage pathogenesis. Diagnosis and treatment of intestinal disorders underlying leaky gut might improve endometrial environment and pregnancy outcome.",
keywords = "Human endometrium, Inflammasome, Intestinal permeability, Recurrent pregnancy loss",
author = "C. Tersigni and S. D'Ippolito and {Di Nicuolo}, F. and R. Marana and V. Valenza and V. Masciullo and F. Scaldaferri and F. Malatacca and C. Waure and A. Gasbarrini and G. Scambia and {Di Simone}, N.",
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T2 - A novel pathogenic model of endometrium inflammation?

AU - Tersigni, C.

AU - D'Ippolito, S.

AU - Di Nicuolo, F.

AU - Marana, R.

AU - Valenza, V.

AU - Masciullo, V.

AU - Scaldaferri, F.

AU - Malatacca, F.

AU - Waure, C.

AU - Gasbarrini, A.

AU - Scambia, G.

AU - Di Simone, N.

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N2 - Background: Recurrent pregnancy loss (RPL) occurs in 3-5% in about 30% of cases no cause can be found. Women with RPL show higher prevalence of undiagnosed gut disorders. Furthermore, in endometrial tissues of RPL women, higher expression of pro-inflammatory cytokines and Nalp-3 inflammasome has been observed. Aim of this study was to investigate whether an abnormal gut permeability might occur in RPL women and allow passage into systemic circulation of pro-inflammatory molecules able to induce endometrial inflammation. Methods: 70 women with idiopathic RPL and 30 healthy women were recruited at the Recurrent Pregnancy Loss Outpatient Unit of the Gemelli Hospital of Rome from March 2013 to February 2017. Enrolled women underwent 51Cr-ethylene-diamine-tetraacetic acid absorption test to evaluate intestinal permeability. Sera obtained from enrolled women were analysed for lipopolysaccharide (LPS) by ELISA. Anxiety and depression state were evaluated by administering STAI-Y and Zung-SDS tests, respectively. Of all recruited individuals, 35 women with idiopathic RPL and 20 healthy controls accepted to undergo diagnostic hysteroscopy and endometrial biopsy. Endometrial lysates were investigated for inflammasome Nalp-3 by Western blot analysis, and caspase-1, IL-1β and IL-18 by ELISA, respectively. Results: Higher prevalence of abnormal intestinal permeability (P<0.0001), increased circulating levels of LPS (P<0.05), anxiety (P<0.05) and depression (P<0.05) were observed in RLP women compared to controls. Endometrial expression of Nalp-3, caspase-1 and IL-1β was significantly increased in RPL group (P<0.0001; P<0.05 and P<0.001, respectively). IL-18 endometrial levels were not found to be higher in RPL cases. Statistically significant association between higher intestinal permeability and abnormally increased expression of endometrial Nalp-3, was observed in RPL (P<0.01). Furthermore, higher LPS serum levels, a bacterial-derived activator of Nalp-3 complex, was shown to be statistically associated to abnormal endometrial expression of Nalp-3 inflammasome (P<0.01) in RPL women. Conclusions: In women with RLP, leaky gut might occur and allow passage into circulation of immune triggers, potentially able to elicit endometrial innate immune response and, thus, to contribute to miscarriage pathogenesis. Diagnosis and treatment of intestinal disorders underlying leaky gut might improve endometrial environment and pregnancy outcome.

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KW - Human endometrium

KW - Inflammasome

KW - Intestinal permeability

KW - Recurrent pregnancy loss

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