Abstract
The authors have investigated the relationships between the active calcium transport across the human red blood cell (RBC) membrane and the RBC aging processes in vivo and in vitro. For the study of this biological system, the authors have determined the active calcium uptake by inside-out membrane vesicles obtained from selected RBC populations. This model provided an optimal way to assess the biochemical and functional responses of the human cell to the oxidative stimulus triggered by the cellular aging processes. The activity of the calcium pump is indeed strictly correlated to the oxidative damage suffered by the RBC, being higher in the aged RBC. It appears that the main controller of the active calcium transport is the age-dependent protein inhibitor of the calcium pump.
| Original language | English |
|---|---|
| Pages (from-to) | 279-286 |
| Number of pages | 8 |
| Journal | Experimental Gerontology |
| Volume | 25 |
| Issue number | 3-4 |
| DOIs | |
| Publication status | Published - 1990 |
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Keywords
- active calcium transport
- calcium homeostasis
- inside-out vesicles
- oxidative damage
- red cell aging
- red cell density
ASJC Scopus subject areas
- Ageing
- Medicine(all)
Cite this
Red cell aging and active calcium transport. / Samaja, Michele; Rubinacci, Alessandro; Motterlini, Roberto; De Ponti, Alessandro; Portinaro, Nicola.
In: Experimental Gerontology, Vol. 25, No. 3-4, 1990, p. 279-286.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Red cell aging and active calcium transport
AU - Samaja, Michele
AU - Rubinacci, Alessandro
AU - Motterlini, Roberto
AU - De Ponti, Alessandro
AU - Portinaro, Nicola
PY - 1990
Y1 - 1990
N2 - The authors have investigated the relationships between the active calcium transport across the human red blood cell (RBC) membrane and the RBC aging processes in vivo and in vitro. For the study of this biological system, the authors have determined the active calcium uptake by inside-out membrane vesicles obtained from selected RBC populations. This model provided an optimal way to assess the biochemical and functional responses of the human cell to the oxidative stimulus triggered by the cellular aging processes. The activity of the calcium pump is indeed strictly correlated to the oxidative damage suffered by the RBC, being higher in the aged RBC. It appears that the main controller of the active calcium transport is the age-dependent protein inhibitor of the calcium pump.
AB - The authors have investigated the relationships between the active calcium transport across the human red blood cell (RBC) membrane and the RBC aging processes in vivo and in vitro. For the study of this biological system, the authors have determined the active calcium uptake by inside-out membrane vesicles obtained from selected RBC populations. This model provided an optimal way to assess the biochemical and functional responses of the human cell to the oxidative stimulus triggered by the cellular aging processes. The activity of the calcium pump is indeed strictly correlated to the oxidative damage suffered by the RBC, being higher in the aged RBC. It appears that the main controller of the active calcium transport is the age-dependent protein inhibitor of the calcium pump.
KW - active calcium transport
KW - calcium homeostasis
KW - inside-out vesicles
KW - oxidative damage
KW - red cell aging
KW - red cell density
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UR - http://www.scopus.com/inward/citedby.url?scp=0025034365&partnerID=8YFLogxK
U2 - 10.1016/0531-5565(90)90063-8
DO - 10.1016/0531-5565(90)90063-8
M3 - Article
C2 - 2226662
AN - SCOPUS:0025034365
VL - 25
SP - 279
EP - 286
JO - Experimental Gerontology
JF - Experimental Gerontology
SN - 0531-5565
IS - 3-4
ER -