Red Cell Alloantibody Screening: Comparative Analysis of Three Different Technologies

Nicoletta Orlando, Maria Bianchi, Caterina Giovanna Valentini, Maddalena Maresca, Giuseppina Massini, Rossana Putzulu, Gina Zini, Luciana Teofili

Research output: Contribution to journalArticlepeer-review


Background: The detection of irregular antibody is a critical issue in the management of red blood cell transfusion according to the Type & Screen (T&S) practice. In order to implement the T&S procedure at our blood bank, we compared three different automated analyzers based on column agglutination technique (CAT) or solid phase red cell adherence assay (SPACA) methods. Methods: Pre-transfusion antibody screening was performed in 986 patients candidate to elective surgery at low risk for red blood cell transfusion. We tested the following kits: the three-cell panel micro-CAT system ID-DiaCell I-II-III (DiaMed), the four-cell panel solid-phase system Capture-R Ready Screen-4 (Immucor), and the four-cell panel micro-CAT system Serascan Diana-4 (Grifols). Positive results were further investigated using corresponding identification panels, and discrepant results were investigated with all the antibody identification systems. Results: Among 986 samples, we observed 967 concordant negative results (98.1%), 8 concordant positive results (0.8% of cases), and 11 discrepant results (1.1%). Among discrepant samples, an alloantibody could been identified in two patents (anti-M, detected by Serascan Diana-4 and ID-DiaCell I, II, III; anti-Kpa, detected by Capture-R Ready Screen-4 and Serascan Diana-4). Conclusion: Among the evaluated technologies, the four-cell panel micro-CAT system displayed the highest sensitivity and specificity with an optimal negative predictive value. These features might be relevant to the routine implementation of the T&S transfusion strategy.

Original languageEnglish
Pages (from-to)179-183
Number of pages5
JournalTransfusion Medicine and Hemotherapy
Issue number3
Publication statusPublished - May 1 2018


  • Diagnostic accuracy
  • Individualized medicine
  • Predictive assays
  • Red blood cell alloantibodies
  • Type & Screen

ASJC Scopus subject areas

  • Immunology and Allergy
  • Hematology


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