Red grape (Vitis vinifera L.) flavonoids down-regulate collagen type III expression after UV-A in primary human dermal blood endothelial cells

Serena Di Francesco, Monica Savio, Nora Bloise, Giovanni Borroni, Lucia Anna Stivala, Riccardo G. Borroni

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Red grape (Vitis vinifera L.) flavonoids including flavan-3-ols (eg, catechin and epicatechin), flavonols (eg, quercetin) and anthocyanins (eg, malvidin) exert anti-inflammatory and antioxidant activities. In the skin they also have a photoprotective action, and their effects have been extensively investigated in keratinocytes, melanocytes and fibroblasts. Despite their known effects also on blood vasculature, little is known on their activities on human dermal blood endothelial cells (HDBECs), which are critically involved in skin homeostasis as well as in the pathogenesis of neoplastic and inflammatory skin diseases. We sought to study the biological effects of selected red grape flavonoids in preventing the consequences of ultraviolet (UV)-A irradiation in vitro. Our results show that red grape flavonoids prevent UV-A-induced sICAM-1 release in HDBECs, suggesting that this cell type could represent an additional target of the anti-inflammatory activity of flavonoids. In addition, flavonoids effectively inhibited UV-A-induced synthesis of collagen type III at both RNA and protein level, indicating that dermal blood microvasculature could be actively involved in ECM remodelling as a consequence of skin photo-ageing, and that this can be prevented by red grape flavonoids.

Original languageEnglish
Pages (from-to)973-980
Number of pages8
JournalExperimental Dermatology
Volume27
Issue number9
DOIs
Publication statusPublished - Sep 1 2018

Fingerprint

Collagen Type III
Endothelial cells
Vitis
Flavonoids
Blood Cells
Blood
Down-Regulation
Endothelial Cells
Skin
Catechin
Anti-Inflammatory Agents
Skin Aging
Military electronic countermeasures
Flavonols
Anthocyanins
Melanocytes
Quercetin
Fibroblasts
Microvessels
Keratinocytes

Keywords

  • dermal microvasculature
  • extracellular matrix
  • flavonoids
  • inflammation
  • ultraviolet radiation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Red grape (Vitis vinifera L.) flavonoids down-regulate collagen type III expression after UV-A in primary human dermal blood endothelial cells. / Di Francesco, Serena; Savio, Monica; Bloise, Nora; Borroni, Giovanni; Stivala, Lucia Anna; Borroni, Riccardo G.

In: Experimental Dermatology, Vol. 27, No. 9, 01.09.2018, p. 973-980.

Research output: Contribution to journalArticle

Di Francesco, Serena ; Savio, Monica ; Bloise, Nora ; Borroni, Giovanni ; Stivala, Lucia Anna ; Borroni, Riccardo G. / Red grape (Vitis vinifera L.) flavonoids down-regulate collagen type III expression after UV-A in primary human dermal blood endothelial cells. In: Experimental Dermatology. 2018 ; Vol. 27, No. 9. pp. 973-980.
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