Redox regulation of T-cell turnover by the p13 protein of human T-cell leukemia virus type 1

Distinct effects in primary versus transformed cells

Micol Silic-Benussi, Ilaria Cavallari, Nicola Vajente, Silvia Vidali, Luigi Chieco-Bianchi, Fabio Di Lisa, Daniela Saggioro, Donna M. D'Agostino, Vincenzo Ciminale

Research output: Contribution to journalArticle

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Abstract

The present study investigated the function of p13, a mitochondrial protein of human T-cell leukemia virus type 1 (HTLV-1). Although necessary for viral propagation in vivo, the mechanism of function of p13 is incompletely understood. Drawing from studies in isolated mitochondria, we analyzed the effects of p13 on mitochondrial reactive oxygen species (ROS) in transformed and primary T cells. In transformed cells (Jurkat, HeLa), p13 did not affect ROS unless the cells were subjected to glucose deprivation, which led to a p13-dependent increase in ROS and cell death. Using RNA interference we confirmed that expression of p13 also influences glucose starvation-induced cell death in the context of HTLV-1-infected cells. ROS measurements showed an increasing gradient from resting to mitogen-activated primary T cells to transformed T cells (Jurkat). Expression of p13 in primary T cells resulted in their activation, an effect that was abrogated by ROS scavengers. These findings suggest that p13 may have a distinct impact on cell turnover depending on the inherent ROS levels; in the context of the HTLV-1 propagation strategy, p13 could increase the pool of "normal" infected cells while culling cells acquiring a transformed phenotype, thus favoring lifelong persistence of the virus in the host.

Original languageEnglish
Pages (from-to)54-62
Number of pages9
JournalBlood
Volume116
Issue number1
DOIs
Publication statusPublished - Jul 8 2010

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Deltaretrovirus
T-cells
Viruses
Oxidation-Reduction
Reactive Oxygen Species
T-Lymphocytes
Proteins
Cell death
Cell Death
Glucose
Jurkat Cells
Mitochondrial Proteins
Mitochondria
Starvation
RNA Interference
Mitogens
HeLa Cells
Phenotype
Chemical activation
RNA

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Redox regulation of T-cell turnover by the p13 protein of human T-cell leukemia virus type 1 : Distinct effects in primary versus transformed cells. / Silic-Benussi, Micol; Cavallari, Ilaria; Vajente, Nicola; Vidali, Silvia; Chieco-Bianchi, Luigi; Di Lisa, Fabio; Saggioro, Daniela; D'Agostino, Donna M.; Ciminale, Vincenzo.

In: Blood, Vol. 116, No. 1, 08.07.2010, p. 54-62.

Research output: Contribution to journalArticle

Silic-Benussi, Micol ; Cavallari, Ilaria ; Vajente, Nicola ; Vidali, Silvia ; Chieco-Bianchi, Luigi ; Di Lisa, Fabio ; Saggioro, Daniela ; D'Agostino, Donna M. ; Ciminale, Vincenzo. / Redox regulation of T-cell turnover by the p13 protein of human T-cell leukemia virus type 1 : Distinct effects in primary versus transformed cells. In: Blood. 2010 ; Vol. 116, No. 1. pp. 54-62.
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