Abstract
We have used the R6/2 mice to study cortico-striatal glutamatergic transmission by microdialysis in freely moving mice. Basal extracellular striatal glutamate concentrations were lower in R6/2 mice at 12 weeks of age, but not at 6 weeks of age, when neurological symptoms start to develop. In contrast, K-induced glutamate release was blunted in the striatum of R6/2 mice at both 6 and 12 weeks of age as compared with age-matched controls. We also found a substantial reduction in striatal pro-BDNF (brain derived neurotrophic factor) levels associated with no changes in the mature form of BDNF, as assessed by immunoblotting, in 12-week-old R6/2 mice, suggesting a reduced turnover rate of BDNF in the striatum of these mice. These data support the hypothesis of a cortico-striatal dysfunction in Huntington's disease.
| Original language | English |
|---|---|
| Pages (from-to) | 1997-2000 |
| Number of pages | 4 |
| Journal | NeuroReport |
| Volume | 18 |
| Issue number | 18 |
| DOIs | |
| Publication status | Published - Dec 2007 |
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Keywords
- BDNF
- Glutamate
- Huntington's disease
- Microdialysis
- R6/2 mouse
- Striatum
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Reduced activity of cortico-striatal fibres in the R6/2 mouse model of Huntington's disease. / Traficante, Anna; Riozzi, Barbara; Cannella, Milena; Rampello, Liborio; Squitieri, Ferdinando; Battaglia, Giuseppe.
In: NeuroReport, Vol. 18, No. 18, 12.2007, p. 1997-2000.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Reduced activity of cortico-striatal fibres in the R6/2 mouse model of Huntington's disease
AU - Traficante, Anna
AU - Riozzi, Barbara
AU - Cannella, Milena
AU - Rampello, Liborio
AU - Squitieri, Ferdinando
AU - Battaglia, Giuseppe
PY - 2007/12
Y1 - 2007/12
N2 - We have used the R6/2 mice to study cortico-striatal glutamatergic transmission by microdialysis in freely moving mice. Basal extracellular striatal glutamate concentrations were lower in R6/2 mice at 12 weeks of age, but not at 6 weeks of age, when neurological symptoms start to develop. In contrast, K-induced glutamate release was blunted in the striatum of R6/2 mice at both 6 and 12 weeks of age as compared with age-matched controls. We also found a substantial reduction in striatal pro-BDNF (brain derived neurotrophic factor) levels associated with no changes in the mature form of BDNF, as assessed by immunoblotting, in 12-week-old R6/2 mice, suggesting a reduced turnover rate of BDNF in the striatum of these mice. These data support the hypothesis of a cortico-striatal dysfunction in Huntington's disease.
AB - We have used the R6/2 mice to study cortico-striatal glutamatergic transmission by microdialysis in freely moving mice. Basal extracellular striatal glutamate concentrations were lower in R6/2 mice at 12 weeks of age, but not at 6 weeks of age, when neurological symptoms start to develop. In contrast, K-induced glutamate release was blunted in the striatum of R6/2 mice at both 6 and 12 weeks of age as compared with age-matched controls. We also found a substantial reduction in striatal pro-BDNF (brain derived neurotrophic factor) levels associated with no changes in the mature form of BDNF, as assessed by immunoblotting, in 12-week-old R6/2 mice, suggesting a reduced turnover rate of BDNF in the striatum of these mice. These data support the hypothesis of a cortico-striatal dysfunction in Huntington's disease.
KW - BDNF
KW - Glutamate
KW - Huntington's disease
KW - Microdialysis
KW - R6/2 mouse
KW - Striatum
UR - http://www.scopus.com/inward/record.url?scp=36248951526&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36248951526&partnerID=8YFLogxK
U2 - 10.1097/WNR.0b013e3282f262ca
DO - 10.1097/WNR.0b013e3282f262ca
M3 - Article
C2 - 18007201
AN - SCOPUS:36248951526
VL - 18
SP - 1997
EP - 2000
JO - NeuroReport
JF - NeuroReport
SN - 0959-4965
IS - 18
ER -