Reduced activity of cortico-striatal fibres in the R6/2 mouse model of Huntington's disease

Anna Traficante, Barbara Riozzi, Milena Cannella, Liborio Rampello, Ferdinando Squitieri, Giuseppe Battaglia

Research output: Contribution to journalArticle

Abstract

We have used the R6/2 mice to study cortico-striatal glutamatergic transmission by microdialysis in freely moving mice. Basal extracellular striatal glutamate concentrations were lower in R6/2 mice at 12 weeks of age, but not at 6 weeks of age, when neurological symptoms start to develop. In contrast, K-induced glutamate release was blunted in the striatum of R6/2 mice at both 6 and 12 weeks of age as compared with age-matched controls. We also found a substantial reduction in striatal pro-BDNF (brain derived neurotrophic factor) levels associated with no changes in the mature form of BDNF, as assessed by immunoblotting, in 12-week-old R6/2 mice, suggesting a reduced turnover rate of BDNF in the striatum of these mice. These data support the hypothesis of a cortico-striatal dysfunction in Huntington's disease.

Original languageEnglish
Pages (from-to)1997-2000
Number of pages4
JournalNeuroReport
Volume18
Issue number18
DOIs
Publication statusPublished - Dec 2007

Fingerprint

Corpus Striatum
Huntington Disease
Brain-Derived Neurotrophic Factor
Glutamic Acid
Microdialysis
Immunoblotting

Keywords

  • BDNF
  • Glutamate
  • Huntington's disease
  • Microdialysis
  • R6/2 mouse
  • Striatum

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Reduced activity of cortico-striatal fibres in the R6/2 mouse model of Huntington's disease. / Traficante, Anna; Riozzi, Barbara; Cannella, Milena; Rampello, Liborio; Squitieri, Ferdinando; Battaglia, Giuseppe.

In: NeuroReport, Vol. 18, No. 18, 12.2007, p. 1997-2000.

Research output: Contribution to journalArticle

@article{686fe168d18343ceb424ab2837875536,
title = "Reduced activity of cortico-striatal fibres in the R6/2 mouse model of Huntington's disease",
abstract = "We have used the R6/2 mice to study cortico-striatal glutamatergic transmission by microdialysis in freely moving mice. Basal extracellular striatal glutamate concentrations were lower in R6/2 mice at 12 weeks of age, but not at 6 weeks of age, when neurological symptoms start to develop. In contrast, K-induced glutamate release was blunted in the striatum of R6/2 mice at both 6 and 12 weeks of age as compared with age-matched controls. We also found a substantial reduction in striatal pro-BDNF (brain derived neurotrophic factor) levels associated with no changes in the mature form of BDNF, as assessed by immunoblotting, in 12-week-old R6/2 mice, suggesting a reduced turnover rate of BDNF in the striatum of these mice. These data support the hypothesis of a cortico-striatal dysfunction in Huntington's disease.",
keywords = "BDNF, Glutamate, Huntington's disease, Microdialysis, R6/2 mouse, Striatum",
author = "Anna Traficante and Barbara Riozzi and Milena Cannella and Liborio Rampello and Ferdinando Squitieri and Giuseppe Battaglia",
year = "2007",
month = "12",
doi = "10.1097/WNR.0b013e3282f262ca",
language = "English",
volume = "18",
pages = "1997--2000",
journal = "NeuroReport",
issn = "0959-4965",
publisher = "Lippincott Williams and Wilkins",
number = "18",

}

TY - JOUR

T1 - Reduced activity of cortico-striatal fibres in the R6/2 mouse model of Huntington's disease

AU - Traficante, Anna

AU - Riozzi, Barbara

AU - Cannella, Milena

AU - Rampello, Liborio

AU - Squitieri, Ferdinando

AU - Battaglia, Giuseppe

PY - 2007/12

Y1 - 2007/12

N2 - We have used the R6/2 mice to study cortico-striatal glutamatergic transmission by microdialysis in freely moving mice. Basal extracellular striatal glutamate concentrations were lower in R6/2 mice at 12 weeks of age, but not at 6 weeks of age, when neurological symptoms start to develop. In contrast, K-induced glutamate release was blunted in the striatum of R6/2 mice at both 6 and 12 weeks of age as compared with age-matched controls. We also found a substantial reduction in striatal pro-BDNF (brain derived neurotrophic factor) levels associated with no changes in the mature form of BDNF, as assessed by immunoblotting, in 12-week-old R6/2 mice, suggesting a reduced turnover rate of BDNF in the striatum of these mice. These data support the hypothesis of a cortico-striatal dysfunction in Huntington's disease.

AB - We have used the R6/2 mice to study cortico-striatal glutamatergic transmission by microdialysis in freely moving mice. Basal extracellular striatal glutamate concentrations were lower in R6/2 mice at 12 weeks of age, but not at 6 weeks of age, when neurological symptoms start to develop. In contrast, K-induced glutamate release was blunted in the striatum of R6/2 mice at both 6 and 12 weeks of age as compared with age-matched controls. We also found a substantial reduction in striatal pro-BDNF (brain derived neurotrophic factor) levels associated with no changes in the mature form of BDNF, as assessed by immunoblotting, in 12-week-old R6/2 mice, suggesting a reduced turnover rate of BDNF in the striatum of these mice. These data support the hypothesis of a cortico-striatal dysfunction in Huntington's disease.

KW - BDNF

KW - Glutamate

KW - Huntington's disease

KW - Microdialysis

KW - R6/2 mouse

KW - Striatum

UR - http://www.scopus.com/inward/record.url?scp=36248951526&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36248951526&partnerID=8YFLogxK

U2 - 10.1097/WNR.0b013e3282f262ca

DO - 10.1097/WNR.0b013e3282f262ca

M3 - Article

C2 - 18007201

AN - SCOPUS:36248951526

VL - 18

SP - 1997

EP - 2000

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 18

ER -