Reduced DNA synthesis in primary cultures of hepatocytes from old mice is restored by thymus grafts

Andrea Basso, Lucio Piantanelli, Giuliana Rossolini, George S. Roth

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We previously observed in vivo that a neonatal thymus grafted into old mice can correct age-related changes such us occurrence of hepatocyte tetraploid nuclei and impaired isoproterenol-induced DNA synthesis in submandibular glands. The aim of the present paper was to study the influence of age and thymus on basal and β-adrenergic-stimulated DNA synthesis using primary cultures of mouse hepatocytes. In the absence of any adrenergic agents, cells from young mice show peak DNA synthesis between 36 and 48 h; old mice show a similar time course, but the peak is significantly reduced statistically. The main result is represented by the behavior of hepatocytes from old thymus-grafted mice, which recover the levels of [3H]-thymidine incorporation toward young-like values. Grafted animals also show a correction of total DNA content that is increased in old mice. The addition of isoproterenol does not modify the DNA synthetic pattern, whereas the antagonist propranolol causes a slight but statistically significant decrease.

Original languageEnglish
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume53
Issue number2
Publication statusPublished - 1998

Fingerprint

Thymus Gland
Hepatocytes
Transplants
DNA
Isoproterenol
Adrenergic Agents
Tetraploidy
Submandibular Gland
Propranolol
Thymidine

ASJC Scopus subject areas

  • Ageing

Cite this

Reduced DNA synthesis in primary cultures of hepatocytes from old mice is restored by thymus grafts. / Basso, Andrea; Piantanelli, Lucio; Rossolini, Giuliana; Roth, George S.

In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences, Vol. 53, No. 2, 1998.

Research output: Contribution to journalArticle

@article{3f2af9dc86de4f6291036752e10f8f34,
title = "Reduced DNA synthesis in primary cultures of hepatocytes from old mice is restored by thymus grafts",
abstract = "We previously observed in vivo that a neonatal thymus grafted into old mice can correct age-related changes such us occurrence of hepatocyte tetraploid nuclei and impaired isoproterenol-induced DNA synthesis in submandibular glands. The aim of the present paper was to study the influence of age and thymus on basal and β-adrenergic-stimulated DNA synthesis using primary cultures of mouse hepatocytes. In the absence of any adrenergic agents, cells from young mice show peak DNA synthesis between 36 and 48 h; old mice show a similar time course, but the peak is significantly reduced statistically. The main result is represented by the behavior of hepatocytes from old thymus-grafted mice, which recover the levels of [3H]-thymidine incorporation toward young-like values. Grafted animals also show a correction of total DNA content that is increased in old mice. The addition of isoproterenol does not modify the DNA synthetic pattern, whereas the antagonist propranolol causes a slight but statistically significant decrease.",
author = "Andrea Basso and Lucio Piantanelli and Giuliana Rossolini and Roth, {George S.}",
year = "1998",
language = "English",
volume = "53",
journal = "Journals of Gerontology - Series A Biological Sciences and Medical Sciences",
issn = "1079-5006",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - Reduced DNA synthesis in primary cultures of hepatocytes from old mice is restored by thymus grafts

AU - Basso, Andrea

AU - Piantanelli, Lucio

AU - Rossolini, Giuliana

AU - Roth, George S.

PY - 1998

Y1 - 1998

N2 - We previously observed in vivo that a neonatal thymus grafted into old mice can correct age-related changes such us occurrence of hepatocyte tetraploid nuclei and impaired isoproterenol-induced DNA synthesis in submandibular glands. The aim of the present paper was to study the influence of age and thymus on basal and β-adrenergic-stimulated DNA synthesis using primary cultures of mouse hepatocytes. In the absence of any adrenergic agents, cells from young mice show peak DNA synthesis between 36 and 48 h; old mice show a similar time course, but the peak is significantly reduced statistically. The main result is represented by the behavior of hepatocytes from old thymus-grafted mice, which recover the levels of [3H]-thymidine incorporation toward young-like values. Grafted animals also show a correction of total DNA content that is increased in old mice. The addition of isoproterenol does not modify the DNA synthetic pattern, whereas the antagonist propranolol causes a slight but statistically significant decrease.

AB - We previously observed in vivo that a neonatal thymus grafted into old mice can correct age-related changes such us occurrence of hepatocyte tetraploid nuclei and impaired isoproterenol-induced DNA synthesis in submandibular glands. The aim of the present paper was to study the influence of age and thymus on basal and β-adrenergic-stimulated DNA synthesis using primary cultures of mouse hepatocytes. In the absence of any adrenergic agents, cells from young mice show peak DNA synthesis between 36 and 48 h; old mice show a similar time course, but the peak is significantly reduced statistically. The main result is represented by the behavior of hepatocytes from old thymus-grafted mice, which recover the levels of [3H]-thymidine incorporation toward young-like values. Grafted animals also show a correction of total DNA content that is increased in old mice. The addition of isoproterenol does not modify the DNA synthetic pattern, whereas the antagonist propranolol causes a slight but statistically significant decrease.

UR - http://www.scopus.com/inward/record.url?scp=0031896929&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031896929&partnerID=8YFLogxK

M3 - Article

C2 - 9520906

AN - SCOPUS:0031896929

VL - 53

JO - Journals of Gerontology - Series A Biological Sciences and Medical Sciences

JF - Journals of Gerontology - Series A Biological Sciences and Medical Sciences

SN - 1079-5006

IS - 2

ER -