Reduced endothelial progenitor cell number and function in inflammatory bowel disease

A possible link to the pathogenesis

Andrea Garolla, Renata Dinc, Davide Checchin, Andrea Biagioli, Luca De Toni, Valentina Nicoletti, Marco Scarpa, Elisa Bolzonello, Giacomo Carlo Sturniolo, Carlo Foresta

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

OBJECTIVES:Circulating endothelial progenitor cells (EPCs) are essential for endothelial repair and vascular healing. Patients with inflammatory bowel disease (IBD) may suffer from endothelial dysfunction. Reduced EPC number, impaired mobilization, or increased EPC apoptosis may be crucial in this phenomenon. The aim of our study was to investigate the number and function of EPCs in patients with IBD and to assess their endothelial function.METHODS:In 100 IBD patients (47 ulcerative colitis (UC) and 53 Crohn's disease (CD)) and 50 healthy controls, EPC number, CXC motif receptor 4 (CXCR4) expression, the percentage of apoptotic circulating EPCs, and the number of colony-forming units were evaluated. Endothelial dysfunction was assessed by luteinizing hormone (LH), follicle stimulating hormone (FSH), and testosterone levels, and in a subgroup of patients, brachial artery flow-mediated dilation (FMD) was measured. Kruskal-Wallis ANOVA (analysis of variance), Mann-Whitney U two-tailed, and Spearman's rank correlation tests were used to assess differences.RESULTS:EPC number was significantly lower in UC patients (39.6 (95% confidence interval (95% CI): 30.7-48.6)) and in CD patients (43.1 (95% CI: 35.9-50.4)) than in healthy controls (97.1 (95% CI: 88.3-105.9)), (P0.001). LH and FSH levels and CXCR4 expression on EPCs did not significantly differ from controls. Testosterone concentrations and FMD were lower in UC patients. Number of apoptotic EPCs was higher in both UC and CD patients with an impaired ability to generate colony in vitro.CONCLUSIONS:We hypothesize that in IBD patients, apoptosis contributes to the reduction of circulating EPC number and to their ability to proliferate in vitro. As this condition represents a risk factor for cardiovascular disease, endothelial function should be evaluated in these patients.

Original languageEnglish
Pages (from-to)2500-2507
Number of pages8
JournalAmerican Journal of Gastroenterology
Volume104
Issue number10
DOIs
Publication statusPublished - Oct 2009

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Inflammatory Bowel Diseases
Cell Count
Ulcerative Colitis
Crohn Disease
Follicle Stimulating Hormone
Confidence Intervals
Luteinizing Hormone
Testosterone
Dilatation
Endothelial Progenitor Cells
Apoptosis
Brachial Artery
Blood Vessels
Analysis of Variance
Cardiovascular Diseases
Stem Cells

ASJC Scopus subject areas

  • Gastroenterology
  • Medicine(all)

Cite this

Reduced endothelial progenitor cell number and function in inflammatory bowel disease : A possible link to the pathogenesis. / Garolla, Andrea; Dinc, Renata; Checchin, Davide; Biagioli, Andrea; De Toni, Luca; Nicoletti, Valentina; Scarpa, Marco; Bolzonello, Elisa; Sturniolo, Giacomo Carlo; Foresta, Carlo.

In: American Journal of Gastroenterology, Vol. 104, No. 10, 10.2009, p. 2500-2507.

Research output: Contribution to journalArticle

Garolla, A, Dinc, R, Checchin, D, Biagioli, A, De Toni, L, Nicoletti, V, Scarpa, M, Bolzonello, E, Sturniolo, GC & Foresta, C 2009, 'Reduced endothelial progenitor cell number and function in inflammatory bowel disease: A possible link to the pathogenesis', American Journal of Gastroenterology, vol. 104, no. 10, pp. 2500-2507. https://doi.org/10.1038/ajg.2009.332
Garolla, Andrea ; Dinc, Renata ; Checchin, Davide ; Biagioli, Andrea ; De Toni, Luca ; Nicoletti, Valentina ; Scarpa, Marco ; Bolzonello, Elisa ; Sturniolo, Giacomo Carlo ; Foresta, Carlo. / Reduced endothelial progenitor cell number and function in inflammatory bowel disease : A possible link to the pathogenesis. In: American Journal of Gastroenterology. 2009 ; Vol. 104, No. 10. pp. 2500-2507.
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abstract = "OBJECTIVES:Circulating endothelial progenitor cells (EPCs) are essential for endothelial repair and vascular healing. Patients with inflammatory bowel disease (IBD) may suffer from endothelial dysfunction. Reduced EPC number, impaired mobilization, or increased EPC apoptosis may be crucial in this phenomenon. The aim of our study was to investigate the number and function of EPCs in patients with IBD and to assess their endothelial function.METHODS:In 100 IBD patients (47 ulcerative colitis (UC) and 53 Crohn's disease (CD)) and 50 healthy controls, EPC number, CXC motif receptor 4 (CXCR4) expression, the percentage of apoptotic circulating EPCs, and the number of colony-forming units were evaluated. Endothelial dysfunction was assessed by luteinizing hormone (LH), follicle stimulating hormone (FSH), and testosterone levels, and in a subgroup of patients, brachial artery flow-mediated dilation (FMD) was measured. Kruskal-Wallis ANOVA (analysis of variance), Mann-Whitney U two-tailed, and Spearman's rank correlation tests were used to assess differences.RESULTS:EPC number was significantly lower in UC patients (39.6 (95{\%} confidence interval (95{\%} CI): 30.7-48.6)) and in CD patients (43.1 (95{\%} CI: 35.9-50.4)) than in healthy controls (97.1 (95{\%} CI: 88.3-105.9)), (P0.001). LH and FSH levels and CXCR4 expression on EPCs did not significantly differ from controls. Testosterone concentrations and FMD were lower in UC patients. Number of apoptotic EPCs was higher in both UC and CD patients with an impaired ability to generate colony in vitro.CONCLUSIONS:We hypothesize that in IBD patients, apoptosis contributes to the reduction of circulating EPC number and to their ability to proliferate in vitro. As this condition represents a risk factor for cardiovascular disease, endothelial function should be evaluated in these patients.",
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T2 - A possible link to the pathogenesis

AU - Garolla, Andrea

AU - Dinc, Renata

AU - Checchin, Davide

AU - Biagioli, Andrea

AU - De Toni, Luca

AU - Nicoletti, Valentina

AU - Scarpa, Marco

AU - Bolzonello, Elisa

AU - Sturniolo, Giacomo Carlo

AU - Foresta, Carlo

PY - 2009/10

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N2 - OBJECTIVES:Circulating endothelial progenitor cells (EPCs) are essential for endothelial repair and vascular healing. Patients with inflammatory bowel disease (IBD) may suffer from endothelial dysfunction. Reduced EPC number, impaired mobilization, or increased EPC apoptosis may be crucial in this phenomenon. The aim of our study was to investigate the number and function of EPCs in patients with IBD and to assess their endothelial function.METHODS:In 100 IBD patients (47 ulcerative colitis (UC) and 53 Crohn's disease (CD)) and 50 healthy controls, EPC number, CXC motif receptor 4 (CXCR4) expression, the percentage of apoptotic circulating EPCs, and the number of colony-forming units were evaluated. Endothelial dysfunction was assessed by luteinizing hormone (LH), follicle stimulating hormone (FSH), and testosterone levels, and in a subgroup of patients, brachial artery flow-mediated dilation (FMD) was measured. Kruskal-Wallis ANOVA (analysis of variance), Mann-Whitney U two-tailed, and Spearman's rank correlation tests were used to assess differences.RESULTS:EPC number was significantly lower in UC patients (39.6 (95% confidence interval (95% CI): 30.7-48.6)) and in CD patients (43.1 (95% CI: 35.9-50.4)) than in healthy controls (97.1 (95% CI: 88.3-105.9)), (P0.001). LH and FSH levels and CXCR4 expression on EPCs did not significantly differ from controls. Testosterone concentrations and FMD were lower in UC patients. Number of apoptotic EPCs was higher in both UC and CD patients with an impaired ability to generate colony in vitro.CONCLUSIONS:We hypothesize that in IBD patients, apoptosis contributes to the reduction of circulating EPC number and to their ability to proliferate in vitro. As this condition represents a risk factor for cardiovascular disease, endothelial function should be evaluated in these patients.

AB - OBJECTIVES:Circulating endothelial progenitor cells (EPCs) are essential for endothelial repair and vascular healing. Patients with inflammatory bowel disease (IBD) may suffer from endothelial dysfunction. Reduced EPC number, impaired mobilization, or increased EPC apoptosis may be crucial in this phenomenon. The aim of our study was to investigate the number and function of EPCs in patients with IBD and to assess their endothelial function.METHODS:In 100 IBD patients (47 ulcerative colitis (UC) and 53 Crohn's disease (CD)) and 50 healthy controls, EPC number, CXC motif receptor 4 (CXCR4) expression, the percentage of apoptotic circulating EPCs, and the number of colony-forming units were evaluated. Endothelial dysfunction was assessed by luteinizing hormone (LH), follicle stimulating hormone (FSH), and testosterone levels, and in a subgroup of patients, brachial artery flow-mediated dilation (FMD) was measured. Kruskal-Wallis ANOVA (analysis of variance), Mann-Whitney U two-tailed, and Spearman's rank correlation tests were used to assess differences.RESULTS:EPC number was significantly lower in UC patients (39.6 (95% confidence interval (95% CI): 30.7-48.6)) and in CD patients (43.1 (95% CI: 35.9-50.4)) than in healthy controls (97.1 (95% CI: 88.3-105.9)), (P0.001). LH and FSH levels and CXCR4 expression on EPCs did not significantly differ from controls. Testosterone concentrations and FMD were lower in UC patients. Number of apoptotic EPCs was higher in both UC and CD patients with an impaired ability to generate colony in vitro.CONCLUSIONS:We hypothesize that in IBD patients, apoptosis contributes to the reduction of circulating EPC number and to their ability to proliferate in vitro. As this condition represents a risk factor for cardiovascular disease, endothelial function should be evaluated in these patients.

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