Reduced expression of CDKN2a/p16(INK4a) in mycosis fungoides

Ketty Peris, Giorgio Stanta, Maria Concetta Fargnoli, Serena Bonin, Andrea Felli, Ada Amantea, Sergio Chimenti

Research output: Contribution to journalArticle

Abstract

Alterations in the CDKN2a gene have been demonstrated in a wide range of human tumors including hematopoietic malignancies. To verify whether altered CDKN2a expression is involved in the pathogenesis of mycosis fungoides (MF), we examined mRNA expression in 20 patients with MF by RT-PCR and dot blot hybridization. CDKN2a mRNA expression was undetectable in 5 of the 20 patients (25%), intermediate in 13 (65%) and high in 2 (10%). Immunohistochemical studies, which were performed in ten patients, revealed that in the four patients showing no mRNA, p16(INK4a) was expressed in <1% of neoplastic lymphocytes whereas in the four patients with an intermediate mRNA level, specific nuclear staining was present in 1-25% of tumor cells. In the two patients with high levels of CDKN2a mRNA, > 25% of neoplastic lymphocytes stained positively. No direct correlation between clinicopathological and molecular findings was evident in our patients. DNA mutational analysis revealed no alterations in a total of six patients examined. Our results indicate that the lack of CDKN2a expression, as found in 25% of the patients, may have a pathogenetic role in MF even though the absence of CDKN2a mRNA was not associated with point mutations or minor gene deletions.

Original languageEnglish
Pages (from-to)207-211
Number of pages5
JournalArchives of Dermatological Research
Volume291
Issue number4
DOIs
Publication statusPublished - 1999

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Mycosis Fungoides
Messenger RNA
DNA Mutational Analysis
Gene Deletion
Hematologic Neoplasms
Point Mutation
Lymphocytes
Polymerase Chain Reaction
Genes

Keywords

  • CDKN2a
  • Mycosis fungoides
  • p16(INK4a)

ASJC Scopus subject areas

  • Dermatology

Cite this

Peris, K., Stanta, G., Fargnoli, M. C., Bonin, S., Felli, A., Amantea, A., & Chimenti, S. (1999). Reduced expression of CDKN2a/p16(INK4a) in mycosis fungoides. Archives of Dermatological Research, 291(4), 207-211. https://doi.org/10.1007/s004030050395

Reduced expression of CDKN2a/p16(INK4a) in mycosis fungoides. / Peris, Ketty; Stanta, Giorgio; Fargnoli, Maria Concetta; Bonin, Serena; Felli, Andrea; Amantea, Ada; Chimenti, Sergio.

In: Archives of Dermatological Research, Vol. 291, No. 4, 1999, p. 207-211.

Research output: Contribution to journalArticle

Peris, K, Stanta, G, Fargnoli, MC, Bonin, S, Felli, A, Amantea, A & Chimenti, S 1999, 'Reduced expression of CDKN2a/p16(INK4a) in mycosis fungoides', Archives of Dermatological Research, vol. 291, no. 4, pp. 207-211. https://doi.org/10.1007/s004030050395
Peris K, Stanta G, Fargnoli MC, Bonin S, Felli A, Amantea A et al. Reduced expression of CDKN2a/p16(INK4a) in mycosis fungoides. Archives of Dermatological Research. 1999;291(4):207-211. https://doi.org/10.1007/s004030050395
Peris, Ketty ; Stanta, Giorgio ; Fargnoli, Maria Concetta ; Bonin, Serena ; Felli, Andrea ; Amantea, Ada ; Chimenti, Sergio. / Reduced expression of CDKN2a/p16(INK4a) in mycosis fungoides. In: Archives of Dermatological Research. 1999 ; Vol. 291, No. 4. pp. 207-211.
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