Reduced expression of the melanocortin-1 receptor in human liver during brain death

Stefano Gatti, Gualtiero Colombo, Flavia Turcatti, Caterina Lonati, Andrea Sordi, Ferruccio Bonino, James M. Lipton, Anna Catania

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: There is evidence that brain death has detrimental effects on peripheral organs. Clinical and experimental studies on organ donors showed marked inflammation in tissue samples of livers and kidneys collected during brain death. The inflammatory reaction is characterized by release of cytokines and inflammatory cell infiltration. Because melanocortins and their receptors are significant modulators of inflammation, we hypothesized that downregulation of melanocortin receptors during brain death could contribute to enhance inflammation. Methods: Using real-time polymerase chain reaction (PCR) analysis, we determined expression of melanocortin receptors in liver biopsies obtained from brain-dead organ donors before cold ischemia and in normal liver tissue during resection of benign focal lesions of the liver. Tissue biopsies were also ana lyzed for expression of intercellular adhesion molecule-1 (ICAM-1), which has a central function in inflammatory cell migration. Results: Expression of melanocortin-1 receptor (MC1R) mRNA was markedly reduced in liver samples obtained from brain-dead organ donors compared to hepatic tissue collected during resection of benign focal lesions of the liver. Conversely, expression of the adhesion molecule ICAM-1 was significantly increased in livers of brain-dead organ donors. Conclusions: Disruption of the endogenous anti-inflammatory circuit based on MC1R could contribute to tissue damage during brain death.

Original languageEnglish
Pages (from-to)51-55
Number of pages5
JournalNeuroImmunoModulation
Volume13
Issue number1
DOIs
Publication statusPublished - Aug 2006

Keywords

  • Brain death
  • Intercellular adhesion molecule-1
  • Liver inflammation
  • Melanocortin-1 receptor

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Immunology

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