Reduced fertility and spermatogenesis defects in mice lacking chromosomal protein Hmgb2

L. Ronfani, M. Ferraguti, L. Croci, C. E. Ovitt, H. R. Schöler, G. G. Consalez, M. E. Bianchi

Research output: Contribution to journalArticle

Abstract

High mobility group 2 protein (Hmgb2) is a member of the HMGB protein family, which includes the ubiquitous Hmgb1 and the embryo-specific Hmgb3. The three proteins are more than 80% identical at the amino acid level and their biochemical properties are indistinguishable. Hmgb1 is an abundant component of all mammalian nuclei and acts as an architectural factor that bends DNA and promotes protein assembly on specific DNA targets. Cells that lack Hmgb1 can survive, although mutant mice die shortly after birth. As Hmgb2 is present in all cultured cells and is abundant in thymus, the preferred source for HMGB proteins, it was considered a ubiquitous variant of Hmgb1. We show that in adult mice Hmgb2 is restricted mainly to lymphoid organs and testes, although it is widely expressed during embryogenesis. Mice that lack Hmgb2 are viable. However, male Hmgb2-/- mice have reduced fertility, that correlates with Sertoli and germ cell degeneration in seminiferous tubules and immotile spermatozoa. Significantly, Hmgb2 is expressed at very high levels in primary spermatocytes, while it is barely detectable in spermatogonia and elongated spermatids. This peculiar pattern of expression and the phenotype of mutants indicate that Hmgb2 has a specialised role in germ cell differentiation.

Original languageEnglish
Pages (from-to)1265-1273
Number of pages9
JournalDevelopment
Volume128
Issue number8
Publication statusPublished - 2001

Fingerprint

Spermatogenesis
Fertility
HMGB Proteins
Germ Cells
Proteins
High Mobility Group Proteins
Spermatogonia
Seminiferous Tubules
Spermatocytes
Spermatids
Sertoli Cells
DNA
Thymus Gland
Embryonic Development
Spermatozoa
Testis
Cell Differentiation
Cultured Cells
Embryonic Structures
Parturition

Keywords

  • Chromatin
  • High mobility group
  • Mouse
  • Testis
  • Transcription

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Reduced fertility and spermatogenesis defects in mice lacking chromosomal protein Hmgb2. / Ronfani, L.; Ferraguti, M.; Croci, L.; Ovitt, C. E.; Schöler, H. R.; Consalez, G. G.; Bianchi, M. E.

In: Development, Vol. 128, No. 8, 2001, p. 1265-1273.

Research output: Contribution to journalArticle

Ronfani, L, Ferraguti, M, Croci, L, Ovitt, CE, Schöler, HR, Consalez, GG & Bianchi, ME 2001, 'Reduced fertility and spermatogenesis defects in mice lacking chromosomal protein Hmgb2', Development, vol. 128, no. 8, pp. 1265-1273.
Ronfani, L. ; Ferraguti, M. ; Croci, L. ; Ovitt, C. E. ; Schöler, H. R. ; Consalez, G. G. ; Bianchi, M. E. / Reduced fertility and spermatogenesis defects in mice lacking chromosomal protein Hmgb2. In: Development. 2001 ; Vol. 128, No. 8. pp. 1265-1273.
@article{dca1213c58874fd987583f65e9443f8c,
title = "Reduced fertility and spermatogenesis defects in mice lacking chromosomal protein Hmgb2",
abstract = "High mobility group 2 protein (Hmgb2) is a member of the HMGB protein family, which includes the ubiquitous Hmgb1 and the embryo-specific Hmgb3. The three proteins are more than 80{\%} identical at the amino acid level and their biochemical properties are indistinguishable. Hmgb1 is an abundant component of all mammalian nuclei and acts as an architectural factor that bends DNA and promotes protein assembly on specific DNA targets. Cells that lack Hmgb1 can survive, although mutant mice die shortly after birth. As Hmgb2 is present in all cultured cells and is abundant in thymus, the preferred source for HMGB proteins, it was considered a ubiquitous variant of Hmgb1. We show that in adult mice Hmgb2 is restricted mainly to lymphoid organs and testes, although it is widely expressed during embryogenesis. Mice that lack Hmgb2 are viable. However, male Hmgb2-/- mice have reduced fertility, that correlates with Sertoli and germ cell degeneration in seminiferous tubules and immotile spermatozoa. Significantly, Hmgb2 is expressed at very high levels in primary spermatocytes, while it is barely detectable in spermatogonia and elongated spermatids. This peculiar pattern of expression and the phenotype of mutants indicate that Hmgb2 has a specialised role in germ cell differentiation.",
keywords = "Chromatin, High mobility group, Mouse, Testis, Transcription",
author = "L. Ronfani and M. Ferraguti and L. Croci and Ovitt, {C. E.} and Sch{\"o}ler, {H. R.} and Consalez, {G. G.} and Bianchi, {M. E.}",
year = "2001",
language = "English",
volume = "128",
pages = "1265--1273",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "8",

}

TY - JOUR

T1 - Reduced fertility and spermatogenesis defects in mice lacking chromosomal protein Hmgb2

AU - Ronfani, L.

AU - Ferraguti, M.

AU - Croci, L.

AU - Ovitt, C. E.

AU - Schöler, H. R.

AU - Consalez, G. G.

AU - Bianchi, M. E.

PY - 2001

Y1 - 2001

N2 - High mobility group 2 protein (Hmgb2) is a member of the HMGB protein family, which includes the ubiquitous Hmgb1 and the embryo-specific Hmgb3. The three proteins are more than 80% identical at the amino acid level and their biochemical properties are indistinguishable. Hmgb1 is an abundant component of all mammalian nuclei and acts as an architectural factor that bends DNA and promotes protein assembly on specific DNA targets. Cells that lack Hmgb1 can survive, although mutant mice die shortly after birth. As Hmgb2 is present in all cultured cells and is abundant in thymus, the preferred source for HMGB proteins, it was considered a ubiquitous variant of Hmgb1. We show that in adult mice Hmgb2 is restricted mainly to lymphoid organs and testes, although it is widely expressed during embryogenesis. Mice that lack Hmgb2 are viable. However, male Hmgb2-/- mice have reduced fertility, that correlates with Sertoli and germ cell degeneration in seminiferous tubules and immotile spermatozoa. Significantly, Hmgb2 is expressed at very high levels in primary spermatocytes, while it is barely detectable in spermatogonia and elongated spermatids. This peculiar pattern of expression and the phenotype of mutants indicate that Hmgb2 has a specialised role in germ cell differentiation.

AB - High mobility group 2 protein (Hmgb2) is a member of the HMGB protein family, which includes the ubiquitous Hmgb1 and the embryo-specific Hmgb3. The three proteins are more than 80% identical at the amino acid level and their biochemical properties are indistinguishable. Hmgb1 is an abundant component of all mammalian nuclei and acts as an architectural factor that bends DNA and promotes protein assembly on specific DNA targets. Cells that lack Hmgb1 can survive, although mutant mice die shortly after birth. As Hmgb2 is present in all cultured cells and is abundant in thymus, the preferred source for HMGB proteins, it was considered a ubiquitous variant of Hmgb1. We show that in adult mice Hmgb2 is restricted mainly to lymphoid organs and testes, although it is widely expressed during embryogenesis. Mice that lack Hmgb2 are viable. However, male Hmgb2-/- mice have reduced fertility, that correlates with Sertoli and germ cell degeneration in seminiferous tubules and immotile spermatozoa. Significantly, Hmgb2 is expressed at very high levels in primary spermatocytes, while it is barely detectable in spermatogonia and elongated spermatids. This peculiar pattern of expression and the phenotype of mutants indicate that Hmgb2 has a specialised role in germ cell differentiation.

KW - Chromatin

KW - High mobility group

KW - Mouse

KW - Testis

KW - Transcription

UR - http://www.scopus.com/inward/record.url?scp=0035036205&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035036205&partnerID=8YFLogxK

M3 - Article

C2 - 11262228

AN - SCOPUS:0035036205

VL - 128

SP - 1265

EP - 1273

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 8

ER -