Reduced fructosamine-3-kinase activity and its mRNA in human distal colorectal carcinoma

M. Notarnicola, Maria G. Caruso, V. Tutino, V. Guerra, S. Frisullo, D. F. Altomare, G. Misciagna

Research output: Contribution to journalArticle

Abstract

Fructosamine-3-Kinase (FN3K) is an enzyme phosphorilating fructoselysine (FL) residues on glycated proteins, resulting in the production of protein-bound FL-3-phosphate. The pathological role of the non-enzymatic modification of proteins by reducing sugars has become increasingly evident in various types of disorders, including the cancer. In this study, our aim was to study FN3K enzyme activity, as well as its mRNA in human colorectal cancer (CRC). Thirty consecutive CRC patients undergoing surgery of the colon were enrolled in the study. FN3K enzymatic activity and gene expression were analyzed using a radiometric assay and quantitative RT-PCR, respectively. FN3K is a functionally active enzyme in human colon tissue, without significant differences between normal mucosa and cancer. The mean level of FN3K mRNA was significantly lower in cancer than in the corresponding normal colorectal mucosa The colorectal tumors located on the left side showed lower levels of both enzymatic activity and mRNA FN3K than tumors located in the right side of colon. This paper is the first studying FN3K enzyme activity in human CRC, showing a significant relationship between enzymatic activity, its mRNA and tumor side.

Original languageEnglish
Pages (from-to)257-262
Number of pages6
JournalGenes and Nutrition
Volume5
Issue number3
DOIs
Publication statusPublished - Sep 2010

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Keywords

  • Colorectal cancer
  • Fructosamine-3-kinase activity
  • Gene expression
  • Glycation

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Genetics

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