Reduced glutathione recovers the impairment of the proliferative response of splenic lymphocytes from vitamin E-deficient rats

C. Pieri, F. Moroni, R. Recchioni

Research output: Contribution to journalArticle

Abstract

The effect of reduced glutathione (GSH) on the Con A induce proliferative response of splenic lymphocytes from rats fed a normal or vitamin E-deficient diet has been investigated. The animals were killed when they were 12 months old and after 11 months of dietary treatment. As was expected, a decreased response, measured in terms of blast transformation or [3H]thymidine incorporation, was observed in vitamin E-deficient animals when compared with the control group. This pattern can be accounted for by the large number of dead cells found in deficient animals. GSH addition into the culture medium resulted in a strong increase of the response in both groups and it eliminated the difference caused by the different dietary regimens. Taking into account that, during proliferation, an increase of respiration occurs which increases the risk of free radical production, present data suggest that GSH may substitute vitamin E in protecting the cells.

Original languageEnglish
Pages (from-to)101-109
Number of pages9
JournalArchives of Gerontology and Geriatrics
Volume17
Issue number2
DOIs
Publication statusPublished - 1993

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Vitamin E
Glutathione
animal
Lymphocytes
media culture
Lymphocyte Activation
Thymidine
proliferation
Free Radicals
Culture Media
Respiration
Group
Cell Count
Diet
Control Groups

Keywords

  • Lymphocyte proliferation
  • Reduced glutathione
  • Vitamin E

ASJC Scopus subject areas

  • Ageing
  • Medicine(all)

Cite this

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abstract = "The effect of reduced glutathione (GSH) on the Con A induce proliferative response of splenic lymphocytes from rats fed a normal or vitamin E-deficient diet has been investigated. The animals were killed when they were 12 months old and after 11 months of dietary treatment. As was expected, a decreased response, measured in terms of blast transformation or [3H]thymidine incorporation, was observed in vitamin E-deficient animals when compared with the control group. This pattern can be accounted for by the large number of dead cells found in deficient animals. GSH addition into the culture medium resulted in a strong increase of the response in both groups and it eliminated the difference caused by the different dietary regimens. Taking into account that, during proliferation, an increase of respiration occurs which increases the risk of free radical production, present data suggest that GSH may substitute vitamin E in protecting the cells.",
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T1 - Reduced glutathione recovers the impairment of the proliferative response of splenic lymphocytes from vitamin E-deficient rats

AU - Pieri, C.

AU - Moroni, F.

AU - Recchioni, R.

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N2 - The effect of reduced glutathione (GSH) on the Con A induce proliferative response of splenic lymphocytes from rats fed a normal or vitamin E-deficient diet has been investigated. The animals were killed when they were 12 months old and after 11 months of dietary treatment. As was expected, a decreased response, measured in terms of blast transformation or [3H]thymidine incorporation, was observed in vitamin E-deficient animals when compared with the control group. This pattern can be accounted for by the large number of dead cells found in deficient animals. GSH addition into the culture medium resulted in a strong increase of the response in both groups and it eliminated the difference caused by the different dietary regimens. Taking into account that, during proliferation, an increase of respiration occurs which increases the risk of free radical production, present data suggest that GSH may substitute vitamin E in protecting the cells.

AB - The effect of reduced glutathione (GSH) on the Con A induce proliferative response of splenic lymphocytes from rats fed a normal or vitamin E-deficient diet has been investigated. The animals were killed when they were 12 months old and after 11 months of dietary treatment. As was expected, a decreased response, measured in terms of blast transformation or [3H]thymidine incorporation, was observed in vitamin E-deficient animals when compared with the control group. This pattern can be accounted for by the large number of dead cells found in deficient animals. GSH addition into the culture medium resulted in a strong increase of the response in both groups and it eliminated the difference caused by the different dietary regimens. Taking into account that, during proliferation, an increase of respiration occurs which increases the risk of free radical production, present data suggest that GSH may substitute vitamin E in protecting the cells.

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