TY - JOUR
T1 - Reduced in vivo oxidative stress following 5-methyltetrahydrofolate supplementation in patients with early-onset thrombosis and 677TT methylenetetrahydrofolate reductase genotype
AU - Coppola, Antonio
AU - D'Angelo, Armando
AU - Fermo, Isabella
AU - Mazzola, Giuseppina
AU - Minno, Matteo Nicola Dario Di
AU - Cajani, Alessia
AU - Sala, Angelo
AU - Folco, Giancarlo
AU - Tremoli, Elena
AU - Minno, Giovanni Di
PY - 2005/10
Y1 - 2005/10
N2 - The protective role of folate in vascular disease has been related to antioxidant effects. In 45 patients with previous early-onset (at age 2α (8-iso-PGF 2α), a reliable marker of oxidative stress. At baseline, patients' fasting total homocysteine (tHcy) was 11.5 μmol/l (geometric mean) and urinary excretion of 8-iso-PGF2α was 304 pg/mg creatinine, with the highest metabolite levels in the lowest quartile of plasma folate distribution (P <0.05). After 5-MTHF supplementation, plasma folate levels increased approximately 13-fold (P <0.0001 versus baseline); tHcy levels (6.7 μmol/l, P <0.0001) and urinary 8-iso-PGF2α (254 pg/mg creatinine, P <0.001) were both significantly lowered, their reduction being proportional to baseline values (r = 0.98 and r = 0.77, respectively) and maximal in patients with the lowest pre-supplementation folate levels (P <0.05). The effects on folate (P <0.0001) and tHcy (P = 0.0004) persisted for at least up to 2 months after withdrawing 5-MTHF. In parallel with long-lasting tHcy-lowering effects, a short-course 5-MTHF supplementation reduces in vivo formation of 8-iso-PGF2α in this population, supporting the antioxidant protective effects of folate in vascular disease.
AB - The protective role of folate in vascular disease has been related to antioxidant effects. In 45 patients with previous early-onset (at age 2α (8-iso-PGF 2α), a reliable marker of oxidative stress. At baseline, patients' fasting total homocysteine (tHcy) was 11.5 μmol/l (geometric mean) and urinary excretion of 8-iso-PGF2α was 304 pg/mg creatinine, with the highest metabolite levels in the lowest quartile of plasma folate distribution (P <0.05). After 5-MTHF supplementation, plasma folate levels increased approximately 13-fold (P <0.0001 versus baseline); tHcy levels (6.7 μmol/l, P <0.0001) and urinary 8-iso-PGF2α (254 pg/mg creatinine, P <0.001) were both significantly lowered, their reduction being proportional to baseline values (r = 0.98 and r = 0.77, respectively) and maximal in patients with the lowest pre-supplementation folate levels (P <0.05). The effects on folate (P <0.0001) and tHcy (P = 0.0004) persisted for at least up to 2 months after withdrawing 5-MTHF. In parallel with long-lasting tHcy-lowering effects, a short-course 5-MTHF supplementation reduces in vivo formation of 8-iso-PGF2α in this population, supporting the antioxidant protective effects of folate in vascular disease.
KW - 5, 10-methylenetetrahydrofolate reductase
KW - 5-methyltetrahydrofolate
KW - 8-iso-PGF
KW - Homocysteine
KW - Oxidative stress
KW - Thrombosis
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U2 - 10.1111/j.1365-2141.2005.05732.x
DO - 10.1111/j.1365-2141.2005.05732.x
M3 - Article
C2 - 16173969
AN - SCOPUS:27744492397
VL - 131
SP - 100
EP - 108
JO - British Journal of Haematology
JF - British Journal of Haematology
SN - 0007-1048
IS - 1
ER -