Reduced in vivo oxidative stress following 5-methyltetrahydrofolate supplementation in patients with early-onset thrombosis and 677TT methylenetetrahydrofolate reductase genotype

Antonio Coppola, Armando D'Angelo, Isabella Fermo, Giuseppina Mazzola, Matteo Nicola Dario Di Minno, Alessia Cajani, Angelo Sala, Giancarlo Folco, Elena Tremoli, Giovanni Di Minno

Research output: Contribution to journalArticlepeer-review

Abstract

The protective role of folate in vascular disease has been related to antioxidant effects. In 45 patients with previous early-onset (at age 2α (8-iso-PGF ), a reliable marker of oxidative stress. At baseline, patients' fasting total homocysteine (tHcy) was 11.5 μmol/l (geometric mean) and urinary excretion of 8-iso-PGF was 304 pg/mg creatinine, with the highest metabolite levels in the lowest quartile of plasma folate distribution (P <0.05). After 5-MTHF supplementation, plasma folate levels increased approximately 13-fold (P <0.0001 versus baseline); tHcy levels (6.7 μmol/l, P <0.0001) and urinary 8-iso-PGF (254 pg/mg creatinine, P <0.001) were both significantly lowered, their reduction being proportional to baseline values (r = 0.98 and r = 0.77, respectively) and maximal in patients with the lowest pre-supplementation folate levels (P <0.05). The effects on folate (P <0.0001) and tHcy (P = 0.0004) persisted for at least up to 2 months after withdrawing 5-MTHF. In parallel with long-lasting tHcy-lowering effects, a short-course 5-MTHF supplementation reduces in vivo formation of 8-iso-PGF in this population, supporting the antioxidant protective effects of folate in vascular disease.

Original languageEnglish
Pages (from-to)100-108
Number of pages9
JournalBritish Journal of Haematology
Volume131
Issue number1
DOIs
Publication statusPublished - Oct 2005

Keywords

  • 5, 10-methylenetetrahydrofolate reductase
  • 5-methyltetrahydrofolate
  • 8-iso-PGF
  • Homocysteine
  • Oxidative stress
  • Thrombosis

ASJC Scopus subject areas

  • Hematology

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