Reduced number and impaired function of circulating γδ T cells in patients with cutaneous primary melanoma

Katy Argentati, Francesca Re, Stefano Serresi, Maria G. Tucci, Beatrice Bartozzi, Giovanni Bernardini, Mauro Provinciali

Research output: Contribution to journalArticle

Abstract

We studied the peripheral representation, in vitro expansion, cytokine production, and cytotoxicity of γδ T lymphocytes from 23 patients with cutaneous primary melanoma and 28 healthy subjects. We demonstrated that the absolute number and the percentage of circulating γδ+ T cells were significantly reduced in melanoma patients in comparison with healthy subjects. The decrease was due to a reduction of Vδ2 T cells, whereas the number of Vδ1 T cells was not affected. As a consequence, the Vδ2/Vδ1 ratio was inverted in melanoma patients. A lower percentage of γδ+ T cells producing tumor necrosis factor-α or interferon-γ was found in melanoma patients. After a 10 d in vitro culture, both the percentage and the expansion index of γδ T cells, and in particular of Vδ2 subset, were significantly reduced in melanoma patients in comparison with healthy subjects. The cytotoxicity of sorted γδ T cells against tumor cell lines and the percentage of γδ T cells producing perforins were preserved in melanoma patients. The numerical and functional impairment of γδ T cells could contribute to the inadequate immune response found in melanoma patients and offers the potentiality for the planning of new approaches of immune therapy of malignant melanoma.

Original languageEnglish
Pages (from-to)829-834
Number of pages6
JournalJournal of Investigative Dermatology
Volume120
Issue number5
DOIs
Publication statusPublished - May 1 2003

Keywords

  • γδ T cells
  • Fluorescence-activated cell sorter
  • Human
  • Melanoma
  • Tumor immunity

ASJC Scopus subject areas

  • Dermatology

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