Abstract
Objective: The aim of this study was to verify whether changes in PDK4 mRNA expression in skeletal muscle in formerly obese subjects who underwent malabsorptive bariatric surgery [bilio-pancreatic diversion (BPD)] might be related to insulin sensitivity improvement, and if these possible modifications might correlate with a reduction of the intramyocytic lipid level. Research Methods and Procedures: Six obese women (body mass index 46.6 ± 8.2 kg/m2) were enrolled in the study. Body composition, euglycemic-hyperinsulinemic clamp and muscle biopsies for skeletal muscle lipid analysis, and semiquantitative reverse transcriptase-polymerase chain reaction were performed before and 3 years after BPD. Results: The average weight loss observed after surgery was ∼42%. Increased glucose uptake was accompanied by a significant decrease of PDK4 mRNA (R2 = 0.71, p <0.001). The amounts of intramyocytic triglycerides correlate directly with PDK4 mRNA (R 2 = 0.87, p = 0.005) and inversely with glucose uptake values (R 2 = 0.75, p <0.001). Discussion: Our results support the concept that a reduced tissue availability of fatty acids consequent to a massive lipid malabsorption influences glucose metabolism acting through the regulation of PDH complex. In fact, as shown in animals, a higher level of FFA availability is likely to induce overexpression of PDK4 also in humans.
| Original language | English |
|---|---|
| Pages (from-to) | 176-182 |
| Number of pages | 7 |
| Journal | Obesity Research |
| Volume | 11 |
| Issue number | 2 |
| Publication status | Published - 2003 |
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Keywords
- Biliopancreatic diversion
- Insulin resistance
- Pyruvate dehydrogenase kinase
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Public Health, Environmental and Occupational Health
- Endocrinology
- Food Science
- Endocrinology, Diabetes and Metabolism
Cite this
Reduced PDK4 expression associates with increased insulin sensitivity in postobese patients. / Rosa, Giuseppina; Di Rocco, Paola; Manco, Melania; Greco, Aldo V.; Castagneto, Marco; Vidal, Hubert; Mingrone, Geltrude.
In: Obesity Research, Vol. 11, No. 2, 2003, p. 176-182.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Reduced PDK4 expression associates with increased insulin sensitivity in postobese patients
AU - Rosa, Giuseppina
AU - Di Rocco, Paola
AU - Manco, Melania
AU - Greco, Aldo V.
AU - Castagneto, Marco
AU - Vidal, Hubert
AU - Mingrone, Geltrude
PY - 2003
Y1 - 2003
N2 - Objective: The aim of this study was to verify whether changes in PDK4 mRNA expression in skeletal muscle in formerly obese subjects who underwent malabsorptive bariatric surgery [bilio-pancreatic diversion (BPD)] might be related to insulin sensitivity improvement, and if these possible modifications might correlate with a reduction of the intramyocytic lipid level. Research Methods and Procedures: Six obese women (body mass index 46.6 ± 8.2 kg/m2) were enrolled in the study. Body composition, euglycemic-hyperinsulinemic clamp and muscle biopsies for skeletal muscle lipid analysis, and semiquantitative reverse transcriptase-polymerase chain reaction were performed before and 3 years after BPD. Results: The average weight loss observed after surgery was ∼42%. Increased glucose uptake was accompanied by a significant decrease of PDK4 mRNA (R2 = 0.71, p <0.001). The amounts of intramyocytic triglycerides correlate directly with PDK4 mRNA (R 2 = 0.87, p = 0.005) and inversely with glucose uptake values (R 2 = 0.75, p <0.001). Discussion: Our results support the concept that a reduced tissue availability of fatty acids consequent to a massive lipid malabsorption influences glucose metabolism acting through the regulation of PDH complex. In fact, as shown in animals, a higher level of FFA availability is likely to induce overexpression of PDK4 also in humans.
AB - Objective: The aim of this study was to verify whether changes in PDK4 mRNA expression in skeletal muscle in formerly obese subjects who underwent malabsorptive bariatric surgery [bilio-pancreatic diversion (BPD)] might be related to insulin sensitivity improvement, and if these possible modifications might correlate with a reduction of the intramyocytic lipid level. Research Methods and Procedures: Six obese women (body mass index 46.6 ± 8.2 kg/m2) were enrolled in the study. Body composition, euglycemic-hyperinsulinemic clamp and muscle biopsies for skeletal muscle lipid analysis, and semiquantitative reverse transcriptase-polymerase chain reaction were performed before and 3 years after BPD. Results: The average weight loss observed after surgery was ∼42%. Increased glucose uptake was accompanied by a significant decrease of PDK4 mRNA (R2 = 0.71, p <0.001). The amounts of intramyocytic triglycerides correlate directly with PDK4 mRNA (R 2 = 0.87, p = 0.005) and inversely with glucose uptake values (R 2 = 0.75, p <0.001). Discussion: Our results support the concept that a reduced tissue availability of fatty acids consequent to a massive lipid malabsorption influences glucose metabolism acting through the regulation of PDH complex. In fact, as shown in animals, a higher level of FFA availability is likely to induce overexpression of PDK4 also in humans.
KW - Biliopancreatic diversion
KW - Insulin resistance
KW - Pyruvate dehydrogenase kinase
UR - http://www.scopus.com/inward/record.url?scp=0042730291&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0042730291&partnerID=8YFLogxK
M3 - Article
C2 - 12582211
AN - SCOPUS:0042730291
VL - 11
SP - 176
EP - 182
JO - Obesity Research
JF - Obesity Research
SN - 1071-7323
IS - 2
ER -