Reduced PDK4 expression associates with increased insulin sensitivity in postobese patients

Giuseppina Rosa, Paola Di Rocco, Melania Manco, Aldo V. Greco, Marco Castagneto, Hubert Vidal, Geltrude Mingrone

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Objective: The aim of this study was to verify whether changes in PDK4 mRNA expression in skeletal muscle in formerly obese subjects who underwent malabsorptive bariatric surgery [bilio-pancreatic diversion (BPD)] might be related to insulin sensitivity improvement, and if these possible modifications might correlate with a reduction of the intramyocytic lipid level. Research Methods and Procedures: Six obese women (body mass index 46.6 ± 8.2 kg/m2) were enrolled in the study. Body composition, euglycemic-hyperinsulinemic clamp and muscle biopsies for skeletal muscle lipid analysis, and semiquantitative reverse transcriptase-polymerase chain reaction were performed before and 3 years after BPD. Results: The average weight loss observed after surgery was ∼42%. Increased glucose uptake was accompanied by a significant decrease of PDK4 mRNA (R2 = 0.71, p <0.001). The amounts of intramyocytic triglycerides correlate directly with PDK4 mRNA (R 2 = 0.87, p = 0.005) and inversely with glucose uptake values (R 2 = 0.75, p <0.001). Discussion: Our results support the concept that a reduced tissue availability of fatty acids consequent to a massive lipid malabsorption influences glucose metabolism acting through the regulation of PDH complex. In fact, as shown in animals, a higher level of FFA availability is likely to induce overexpression of PDK4 also in humans.

Original languageEnglish
Pages (from-to)176-182
Number of pages7
JournalObesity Research
Volume11
Issue number2
Publication statusPublished - 2003

Fingerprint

Biliopancreatic Diversion
insulin resistance
Insulin Resistance
Lipids
Glucose
Messenger RNA
glucose
skeletal muscle
Skeletal Muscle
lipids
bariatric surgery
uptake mechanisms
Glucose Clamp Technique
Bariatric Surgery
malabsorption
research methods
Body Composition
Reverse Transcriptase Polymerase Chain Reaction
body composition
body mass index

Keywords

  • Biliopancreatic diversion
  • Insulin resistance
  • Pyruvate dehydrogenase kinase

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Public Health, Environmental and Occupational Health
  • Endocrinology
  • Food Science
  • Endocrinology, Diabetes and Metabolism

Cite this

Rosa, G., Di Rocco, P., Manco, M., Greco, A. V., Castagneto, M., Vidal, H., & Mingrone, G. (2003). Reduced PDK4 expression associates with increased insulin sensitivity in postobese patients. Obesity Research, 11(2), 176-182.

Reduced PDK4 expression associates with increased insulin sensitivity in postobese patients. / Rosa, Giuseppina; Di Rocco, Paola; Manco, Melania; Greco, Aldo V.; Castagneto, Marco; Vidal, Hubert; Mingrone, Geltrude.

In: Obesity Research, Vol. 11, No. 2, 2003, p. 176-182.

Research output: Contribution to journalArticle

Rosa, G, Di Rocco, P, Manco, M, Greco, AV, Castagneto, M, Vidal, H & Mingrone, G 2003, 'Reduced PDK4 expression associates with increased insulin sensitivity in postobese patients', Obesity Research, vol. 11, no. 2, pp. 176-182.
Rosa G, Di Rocco P, Manco M, Greco AV, Castagneto M, Vidal H et al. Reduced PDK4 expression associates with increased insulin sensitivity in postobese patients. Obesity Research. 2003;11(2):176-182.
Rosa, Giuseppina ; Di Rocco, Paola ; Manco, Melania ; Greco, Aldo V. ; Castagneto, Marco ; Vidal, Hubert ; Mingrone, Geltrude. / Reduced PDK4 expression associates with increased insulin sensitivity in postobese patients. In: Obesity Research. 2003 ; Vol. 11, No. 2. pp. 176-182.
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