Reduced priming effect of interleukin 3 on IgE mediated basophil histamine release in patients with systemic sclerosis

Alberto Tedeschi, Claudia Salmaso, Nunzio Milazzo, Antonio Miadonna

Research output: Contribution to journalArticle

Abstract

Objective. To determine the capacity of interleukin 3 (IL-3) to induce and enhance basophil histamine release in patients with systemic sclerosis (SSc). Methods. Leukocyte suspensions prepared by dextran sedimentation of peripheral venous blood were stimulated with anti-IgE (10 μg/ml), N-formyl- methionyl-leucyl-phenylalanine (fMLP, 1 μM), and IL-3 (0.1 to 10 ng/ml). The priming effect of IL-3 on anti-IgE and fMLP induced histamine release was evaluated. Histamine release was measured by an automated fluorometric method. Results. No significant difference was found between patients with SSc (n = 12) and control subjects (n = 16) regarding spontaneous IL-3 and fMLP induced histamine release. IL-3 was a weak basophil agonist in both populations, since net histamine release did not exceed 10% of total histamine content in any case. Anti-IgE induced histamine release was lower in patients with SSc than in controls (13.09 ± 4.8 vs 30.2 ± 6.2%; p = 0.048). IL-3 enhanced anti-IgE and fMLP induced histamine release in a dose dependent fashion. However, the enhancement of anti-IgE induced histamine release by IL-3 was significantly lower in patients with SSc than in controls (p <0.01 at 0.1 ng/ml IL-3; p <0.05 at 1 and 10 ng/ml IL-3). In contrast, no difference was found between the 2 populations regarding the enhancement of fMLP induced histamine release by IL-3. Conclusion. Basophil response to anti-IgE and the priming effect of IL-3 on IgE mediated basophil histamine release are lower in patients with SSc than in controls. These alterations could be related to chronic activation of the IgE/basophil system in patients with SSc.

Original languageEnglish
Pages (from-to)1320-1324
Number of pages5
JournalJournal of Rheumatology
Volume25
Issue number7
Publication statusPublished - Jul 1998

Keywords

  • Basophil
  • Histamine release
  • Interleukin 3
  • Systemic sclerosis

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

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