Reduction and shaping of graphene-oxide by laser-printing for controlled bone tissue regeneration and bacterial killing

Valentina Palmieri, Marta Barba, Lorena Di Pietro, Silvia Gentilini, Maria Chiara Braidotti, Carlotta Ciancico, Francesca Bugli, Gabriele Ciasca, Rosanna Larciprete, Wanda Lattanzi, Maurizio Sanguinetti, Marco De Spirito, Claudio Conti, Massimiliano Papi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Graphene and graphene oxide (GO) are capable of inducing stem cells differentiation into bone tissue with variable efficacy depending on reductive state of the material. Thus, modulation of osteogenic process and of bone mineral density distribution is theoretically possible by controlling the GO oxidative state. In this study, we laser-printed GO surfaces in order to obtain both a local photo-thermal GO reduction and the formation of nano-wrinkles along precise geometric pattern. Initially, after cells adhered on the surface, stem cells migrated and accumulated on the reduced and wrinkled surface. When the local density of the stem cells on the reduced stripes was high, cells started to proliferate and occupy the oxidized/flat area. The designed surfaces morphology guided stem cell orientation and the reduction accelerated differentiation. Furthermore the reduced sharp nano-wrinkles were able to enhance the GO antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA), a common cause of prosthetic joints infections. This strategy can offer a revolution in present and future trends of scaffolds design for regenerative medicine.

Original languageEnglish
Article number015027
Journal2D Materials
Volume5
Issue number1
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Tissue regeneration
Graphite
regeneration
printing
Oxides
Graphene
bones
Printing
graphene
Bone
stem cells
Stem cells
oxides
Lasers
lasers
Methicillin
staphylococcus
infectious diseases
Prosthetics
cells

Keywords

  • antibacterial
  • graphene
  • laser pattern
  • osteogenesis
  • stem cell
  • tailored medicine

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Palmieri, V., Barba, M., Di Pietro, L., Gentilini, S., Braidotti, M. C., Ciancico, C., ... Papi, M. (2018). Reduction and shaping of graphene-oxide by laser-printing for controlled bone tissue regeneration and bacterial killing. 2D Materials, 5(1), [015027]. https://doi.org/10.1088/2053-1583/aa9ca7

Reduction and shaping of graphene-oxide by laser-printing for controlled bone tissue regeneration and bacterial killing. / Palmieri, Valentina; Barba, Marta; Di Pietro, Lorena; Gentilini, Silvia; Braidotti, Maria Chiara; Ciancico, Carlotta; Bugli, Francesca; Ciasca, Gabriele; Larciprete, Rosanna; Lattanzi, Wanda; Sanguinetti, Maurizio; De Spirito, Marco; Conti, Claudio; Papi, Massimiliano.

In: 2D Materials, Vol. 5, No. 1, 015027, 01.01.2018.

Research output: Contribution to journalArticle

Palmieri, V, Barba, M, Di Pietro, L, Gentilini, S, Braidotti, MC, Ciancico, C, Bugli, F, Ciasca, G, Larciprete, R, Lattanzi, W, Sanguinetti, M, De Spirito, M, Conti, C & Papi, M 2018, 'Reduction and shaping of graphene-oxide by laser-printing for controlled bone tissue regeneration and bacterial killing', 2D Materials, vol. 5, no. 1, 015027. https://doi.org/10.1088/2053-1583/aa9ca7
Palmieri, Valentina ; Barba, Marta ; Di Pietro, Lorena ; Gentilini, Silvia ; Braidotti, Maria Chiara ; Ciancico, Carlotta ; Bugli, Francesca ; Ciasca, Gabriele ; Larciprete, Rosanna ; Lattanzi, Wanda ; Sanguinetti, Maurizio ; De Spirito, Marco ; Conti, Claudio ; Papi, Massimiliano. / Reduction and shaping of graphene-oxide by laser-printing for controlled bone tissue regeneration and bacterial killing. In: 2D Materials. 2018 ; Vol. 5, No. 1.
@article{945edca843f6440881527a8d2315c996,
title = "Reduction and shaping of graphene-oxide by laser-printing for controlled bone tissue regeneration and bacterial killing",
abstract = "Graphene and graphene oxide (GO) are capable of inducing stem cells differentiation into bone tissue with variable efficacy depending on reductive state of the material. Thus, modulation of osteogenic process and of bone mineral density distribution is theoretically possible by controlling the GO oxidative state. In this study, we laser-printed GO surfaces in order to obtain both a local photo-thermal GO reduction and the formation of nano-wrinkles along precise geometric pattern. Initially, after cells adhered on the surface, stem cells migrated and accumulated on the reduced and wrinkled surface. When the local density of the stem cells on the reduced stripes was high, cells started to proliferate and occupy the oxidized/flat area. The designed surfaces morphology guided stem cell orientation and the reduction accelerated differentiation. Furthermore the reduced sharp nano-wrinkles were able to enhance the GO antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA), a common cause of prosthetic joints infections. This strategy can offer a revolution in present and future trends of scaffolds design for regenerative medicine.",
keywords = "antibacterial, graphene, laser pattern, osteogenesis, stem cell, tailored medicine",
author = "Valentina Palmieri and Marta Barba and {Di Pietro}, Lorena and Silvia Gentilini and Braidotti, {Maria Chiara} and Carlotta Ciancico and Francesca Bugli and Gabriele Ciasca and Rosanna Larciprete and Wanda Lattanzi and Maurizio Sanguinetti and {De Spirito}, Marco and Claudio Conti and Massimiliano Papi",
year = "2018",
month = "1",
day = "1",
doi = "10.1088/2053-1583/aa9ca7",
language = "English",
volume = "5",
journal = "2D Materials",
issn = "2053-1583",
publisher = "IOP Publishing Ltd.",
number = "1",

}

TY - JOUR

T1 - Reduction and shaping of graphene-oxide by laser-printing for controlled bone tissue regeneration and bacterial killing

AU - Palmieri, Valentina

AU - Barba, Marta

AU - Di Pietro, Lorena

AU - Gentilini, Silvia

AU - Braidotti, Maria Chiara

AU - Ciancico, Carlotta

AU - Bugli, Francesca

AU - Ciasca, Gabriele

AU - Larciprete, Rosanna

AU - Lattanzi, Wanda

AU - Sanguinetti, Maurizio

AU - De Spirito, Marco

AU - Conti, Claudio

AU - Papi, Massimiliano

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Graphene and graphene oxide (GO) are capable of inducing stem cells differentiation into bone tissue with variable efficacy depending on reductive state of the material. Thus, modulation of osteogenic process and of bone mineral density distribution is theoretically possible by controlling the GO oxidative state. In this study, we laser-printed GO surfaces in order to obtain both a local photo-thermal GO reduction and the formation of nano-wrinkles along precise geometric pattern. Initially, after cells adhered on the surface, stem cells migrated and accumulated on the reduced and wrinkled surface. When the local density of the stem cells on the reduced stripes was high, cells started to proliferate and occupy the oxidized/flat area. The designed surfaces morphology guided stem cell orientation and the reduction accelerated differentiation. Furthermore the reduced sharp nano-wrinkles were able to enhance the GO antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA), a common cause of prosthetic joints infections. This strategy can offer a revolution in present and future trends of scaffolds design for regenerative medicine.

AB - Graphene and graphene oxide (GO) are capable of inducing stem cells differentiation into bone tissue with variable efficacy depending on reductive state of the material. Thus, modulation of osteogenic process and of bone mineral density distribution is theoretically possible by controlling the GO oxidative state. In this study, we laser-printed GO surfaces in order to obtain both a local photo-thermal GO reduction and the formation of nano-wrinkles along precise geometric pattern. Initially, after cells adhered on the surface, stem cells migrated and accumulated on the reduced and wrinkled surface. When the local density of the stem cells on the reduced stripes was high, cells started to proliferate and occupy the oxidized/flat area. The designed surfaces morphology guided stem cell orientation and the reduction accelerated differentiation. Furthermore the reduced sharp nano-wrinkles were able to enhance the GO antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA), a common cause of prosthetic joints infections. This strategy can offer a revolution in present and future trends of scaffolds design for regenerative medicine.

KW - antibacterial

KW - graphene

KW - laser pattern

KW - osteogenesis

KW - stem cell

KW - tailored medicine

UR - http://www.scopus.com/inward/record.url?scp=85040097299&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85040097299&partnerID=8YFLogxK

U2 - 10.1088/2053-1583/aa9ca7

DO - 10.1088/2053-1583/aa9ca7

M3 - Article

AN - SCOPUS:85040097299

VL - 5

JO - 2D Materials

JF - 2D Materials

SN - 2053-1583

IS - 1

M1 - 015027

ER -