Reduction of IFN-γ and IL-2 production by peripheral lymphocytes of HIV-exposed seronegative subjects

Emanuele Nicastri, Loredana Sarmati, Lucia Ercoli, Giorgio Mancino, Ettore D'Ambrosio, Gabriella D'Ettorre, Claudio M. Mastroianni, Vincenzo Vullo, Massimo Andreoni

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objectives: In order to evaluate the role played by cytokine profile as a co-factor involved in the resistance to HIV infection in couples serodiscordant for HIV, we studied HIV-seronegative subjects with multiple unprotected sexual exposures. Design and methods: Twenty-one HIV-exposed seronegative subjects (HEPS), their 21 HIV-seropositive partners and 10 HIV-seronegative unexposed individuals were studied for T helper (Th) types 1-2 cell pattern and CCR5 receptor. Results: Twelve out of 21 HIV-seropositive partners of HEPS showed a CD4 cell count below 200 lymphocyte/μl. HIV strains were isolated from peripheral blood mononuclear cells (PBMC) in 17 patients (81%): seven subjects with syncytium-inducing strains and 10 with non-syncytium-inducing isolates. Low Th1 cytokine production and high levels of IL-4 were detected in HIV-seropositive subjects. A significant reduction of IL-2 and IFN-γ expression in the CD4 and CD8 cells of HEPS was found compared with HIV-seronegative unexposed individuals. Similar levels of low IL-4 were present in both HEPS and controls. The partial deletion of a single allele (wild type/Δ32) of CCR5 was found in only one HEPS. Conclusion: The downregulated Th1 profile we observed in HEPS could be related to a cellular anergy state with a protective role in the transmission rate of HIV. Low levels of IL-2 and IFN-γ could be involved in a low-grade activation state of CD4 lymphocytes. A decrease of IFN-γ levels could render macrophage cells incapable of antigen presentation, thus resulting in a reduction of the cell-to-cell spread of infection.

Original languageEnglish
Pages (from-to)1333-1336
Number of pages4
JournalAIDS (London, England)
Volume13
Issue number11
DOIs
Publication statusPublished - 1999

Fingerprint

Interleukin-2
HIV
Lymphocytes
Interleukin-4
CCR5 Receptors
Cytokines
Th2 Cells
Th1 Cells
Antigen Presentation
Giant Cells
CD4 Lymphocyte Count
HIV Infections
Blood Cells
Down-Regulation
Alleles
Macrophages

Keywords

  • HIV-serodiscordant couples
  • Intracellular cytokines

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Reduction of IFN-γ and IL-2 production by peripheral lymphocytes of HIV-exposed seronegative subjects. / Nicastri, Emanuele; Sarmati, Loredana; Ercoli, Lucia; Mancino, Giorgio; D'Ambrosio, Ettore; D'Ettorre, Gabriella; Mastroianni, Claudio M.; Vullo, Vincenzo; Andreoni, Massimo.

In: AIDS (London, England), Vol. 13, No. 11, 1999, p. 1333-1336.

Research output: Contribution to journalArticle

Nicastri, E, Sarmati, L, Ercoli, L, Mancino, G, D'Ambrosio, E, D'Ettorre, G, Mastroianni, CM, Vullo, V & Andreoni, M 1999, 'Reduction of IFN-γ and IL-2 production by peripheral lymphocytes of HIV-exposed seronegative subjects', AIDS (London, England), vol. 13, no. 11, pp. 1333-1336. https://doi.org/10.1097/00002030-199907300-00009
Nicastri, Emanuele ; Sarmati, Loredana ; Ercoli, Lucia ; Mancino, Giorgio ; D'Ambrosio, Ettore ; D'Ettorre, Gabriella ; Mastroianni, Claudio M. ; Vullo, Vincenzo ; Andreoni, Massimo. / Reduction of IFN-γ and IL-2 production by peripheral lymphocytes of HIV-exposed seronegative subjects. In: AIDS (London, England). 1999 ; Vol. 13, No. 11. pp. 1333-1336.
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AU - Sarmati, Loredana

AU - Ercoli, Lucia

AU - Mancino, Giorgio

AU - D'Ambrosio, Ettore

AU - D'Ettorre, Gabriella

AU - Mastroianni, Claudio M.

AU - Vullo, Vincenzo

AU - Andreoni, Massimo

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N2 - Objectives: In order to evaluate the role played by cytokine profile as a co-factor involved in the resistance to HIV infection in couples serodiscordant for HIV, we studied HIV-seronegative subjects with multiple unprotected sexual exposures. Design and methods: Twenty-one HIV-exposed seronegative subjects (HEPS), their 21 HIV-seropositive partners and 10 HIV-seronegative unexposed individuals were studied for T helper (Th) types 1-2 cell pattern and CCR5 receptor. Results: Twelve out of 21 HIV-seropositive partners of HEPS showed a CD4 cell count below 200 lymphocyte/μl. HIV strains were isolated from peripheral blood mononuclear cells (PBMC) in 17 patients (81%): seven subjects with syncytium-inducing strains and 10 with non-syncytium-inducing isolates. Low Th1 cytokine production and high levels of IL-4 were detected in HIV-seropositive subjects. A significant reduction of IL-2 and IFN-γ expression in the CD4 and CD8 cells of HEPS was found compared with HIV-seronegative unexposed individuals. Similar levels of low IL-4 were present in both HEPS and controls. The partial deletion of a single allele (wild type/Δ32) of CCR5 was found in only one HEPS. Conclusion: The downregulated Th1 profile we observed in HEPS could be related to a cellular anergy state with a protective role in the transmission rate of HIV. Low levels of IL-2 and IFN-γ could be involved in a low-grade activation state of CD4 lymphocytes. A decrease of IFN-γ levels could render macrophage cells incapable of antigen presentation, thus resulting in a reduction of the cell-to-cell spread of infection.

AB - Objectives: In order to evaluate the role played by cytokine profile as a co-factor involved in the resistance to HIV infection in couples serodiscordant for HIV, we studied HIV-seronegative subjects with multiple unprotected sexual exposures. Design and methods: Twenty-one HIV-exposed seronegative subjects (HEPS), their 21 HIV-seropositive partners and 10 HIV-seronegative unexposed individuals were studied for T helper (Th) types 1-2 cell pattern and CCR5 receptor. Results: Twelve out of 21 HIV-seropositive partners of HEPS showed a CD4 cell count below 200 lymphocyte/μl. HIV strains were isolated from peripheral blood mononuclear cells (PBMC) in 17 patients (81%): seven subjects with syncytium-inducing strains and 10 with non-syncytium-inducing isolates. Low Th1 cytokine production and high levels of IL-4 were detected in HIV-seropositive subjects. A significant reduction of IL-2 and IFN-γ expression in the CD4 and CD8 cells of HEPS was found compared with HIV-seronegative unexposed individuals. Similar levels of low IL-4 were present in both HEPS and controls. The partial deletion of a single allele (wild type/Δ32) of CCR5 was found in only one HEPS. Conclusion: The downregulated Th1 profile we observed in HEPS could be related to a cellular anergy state with a protective role in the transmission rate of HIV. Low levels of IL-2 and IFN-γ could be involved in a low-grade activation state of CD4 lymphocytes. A decrease of IFN-γ levels could render macrophage cells incapable of antigen presentation, thus resulting in a reduction of the cell-to-cell spread of infection.

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