Abstract
An increased cytokine production, correlated with long term complications of uremic disease, has been described during hemodialysis. To identify possible differences in the cytokine release of differently sterilized membranes, we enrolled six uremic patients on chronic hemodialysis. The patients underwent dialysis with ETO-sterilized low-flux polysulphone membranes (F6, Fresenius AG) for at least three months (A 1), they were then switched to steam-sterilized polysulphone membranes (F6-HPS Fresenius AG) and further evaluations after one (B1) and two months (B2) were carried out. A final evaluation (A2) was made one month after switching back to F6 dialyzers. At each time period, samples were drawn to measure IL-1β released by cultured mononuclear cells (MN). Moreover, dialysate samples were collected to test endotoxin levels. C3a and C5a levels were assessed at 0, 5, 15 and 60 rain from starting hemodialysis. Anti-ETO IgE levels were also assayed at A1, B1 and A2. The LAL test revealed a good quality dialysate. The mean pre-dialysis IL-1β levels were 215 pg/million cells at A 1; falling to 49 at B1, and 54 at B2 (p
| Original language | English |
|---|---|
| Pages (from-to) | 210-215 |
| Number of pages | 6 |
| Journal | International Journal of Artificial Organs |
| Volume | 21 |
| Issue number | 4 |
| Publication status | Published - 1998 |
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Keywords
- Cytokines
- Ethylene oxide
- Hemodialysis
- Low-flux polysulphone
- Steam sterilization
ASJC Scopus subject areas
- Biophysics
Cite this
Reduction of mononuclear cytokine production in hemodialysis patients treated with steam-sterilized low-flux polysulphone membranes. / Aucella, F.; Vigilante, M.; Grandone, E.; Colaizzo, D.; Margaglione, M.; Modoni, S.; Orlandini, G.; Stallone, C.
In: International Journal of Artificial Organs, Vol. 21, No. 4, 1998, p. 210-215.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Reduction of mononuclear cytokine production in hemodialysis patients treated with steam-sterilized low-flux polysulphone membranes
AU - Aucella, F.
AU - Vigilante, M.
AU - Grandone, E.
AU - Colaizzo, D.
AU - Margaglione, M.
AU - Modoni, S.
AU - Orlandini, G.
AU - Stallone, C.
PY - 1998
Y1 - 1998
N2 - An increased cytokine production, correlated with long term complications of uremic disease, has been described during hemodialysis. To identify possible differences in the cytokine release of differently sterilized membranes, we enrolled six uremic patients on chronic hemodialysis. The patients underwent dialysis with ETO-sterilized low-flux polysulphone membranes (F6, Fresenius AG) for at least three months (A 1), they were then switched to steam-sterilized polysulphone membranes (F6-HPS Fresenius AG) and further evaluations after one (B1) and two months (B2) were carried out. A final evaluation (A2) was made one month after switching back to F6 dialyzers. At each time period, samples were drawn to measure IL-1β released by cultured mononuclear cells (MN). Moreover, dialysate samples were collected to test endotoxin levels. C3a and C5a levels were assessed at 0, 5, 15 and 60 rain from starting hemodialysis. Anti-ETO IgE levels were also assayed at A1, B1 and A2. The LAL test revealed a good quality dialysate. The mean pre-dialysis IL-1β levels were 215 pg/million cells at A 1; falling to 49 at B1, and 54 at B2 (p
AB - An increased cytokine production, correlated with long term complications of uremic disease, has been described during hemodialysis. To identify possible differences in the cytokine release of differently sterilized membranes, we enrolled six uremic patients on chronic hemodialysis. The patients underwent dialysis with ETO-sterilized low-flux polysulphone membranes (F6, Fresenius AG) for at least three months (A 1), they were then switched to steam-sterilized polysulphone membranes (F6-HPS Fresenius AG) and further evaluations after one (B1) and two months (B2) were carried out. A final evaluation (A2) was made one month after switching back to F6 dialyzers. At each time period, samples were drawn to measure IL-1β released by cultured mononuclear cells (MN). Moreover, dialysate samples were collected to test endotoxin levels. C3a and C5a levels were assessed at 0, 5, 15 and 60 rain from starting hemodialysis. Anti-ETO IgE levels were also assayed at A1, B1 and A2. The LAL test revealed a good quality dialysate. The mean pre-dialysis IL-1β levels were 215 pg/million cells at A 1; falling to 49 at B1, and 54 at B2 (p
KW - Cytokines
KW - Ethylene oxide
KW - Hemodialysis
KW - Low-flux polysulphone
KW - Steam sterilization
UR - http://www.scopus.com/inward/record.url?scp=0031866120&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031866120&partnerID=8YFLogxK
M3 - Article
VL - 21
SP - 210
EP - 215
JO - International Journal of Artificial Organs
JF - International Journal of Artificial Organs
SN - 0391-3988
IS - 4
ER -