Reduction of mononuclear cytokine production in hemodialysis patients treated with steam-sterilized low-flux polysulphone membranes

F. Aucella, M. Vigilante, E. Grandone, D. Colaizzo, M. Margaglione, S. Modoni, G. Orlandini, C. Stallone

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

An increased cytokine production, correlated with long term complications of uremic disease, has been described during hemodialysis. To identify possible differences in the cytokine release of differently sterilized membranes, we enrolled six uremic patients on chronic hemodialysis. The patients underwent dialysis with ETO-sterilized low-flux polysulphone membranes (F6, Fresenius AG) for at least three months (A 1), they were then switched to steam-sterilized polysulphone membranes (F6-HPS Fresenius AG) and further evaluations after one (B1) and two months (B2) were carried out. A final evaluation (A2) was made one month after switching back to F6 dialyzers. At each time period, samples were drawn to measure IL-1β released by cultured mononuclear cells (MN). Moreover, dialysate samples were collected to test endotoxin levels. C3a and C5a levels were assessed at 0, 5, 15 and 60 rain from starting hemodialysis. Anti-ETO IgE levels were also assayed at A1, B1 and A2. The LAL test revealed a good quality dialysate. The mean pre-dialysis IL-1β levels were 215 pg/million cells at A 1; falling to 49 at B1, and 54 at B2 (p

Original languageEnglish
Pages (from-to)210-215
Number of pages6
JournalInternational Journal of Artificial Organs
Volume21
Issue number4
Publication statusPublished - 1998

Fingerprint

Polysulfones
Steam
Renal Dialysis
Dialysis
Dialysis Solutions
Cytokines
Fluxes
Membranes
Interleukin-1
varespladib methyl
Rain
Endotoxins
Immunoglobulin E
Cultured Cells
polysulfone P 1700

Keywords

  • Cytokines
  • Ethylene oxide
  • Hemodialysis
  • Low-flux polysulphone
  • Steam sterilization

ASJC Scopus subject areas

  • Biophysics

Cite this

Reduction of mononuclear cytokine production in hemodialysis patients treated with steam-sterilized low-flux polysulphone membranes. / Aucella, F.; Vigilante, M.; Grandone, E.; Colaizzo, D.; Margaglione, M.; Modoni, S.; Orlandini, G.; Stallone, C.

In: International Journal of Artificial Organs, Vol. 21, No. 4, 1998, p. 210-215.

Research output: Contribution to journalArticle

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