Abstract
Calcium dobesilate stabilizes blood-retinal barrier in patients with diabetic retinopathy and possesses antioxidant properties in the retinas of rats with streptozotocin-induced diabetes, exposed ex vivo to ischemia-reperfusion. Here we investigated the action of calcium dobesilate on retinal albumin leakage in streptozotocin-diabetic rats, together with relevant in vivo retinal antioxidant and permeability markers, i.e., carboxymethyl-lysine-advanced glycation end product (CML-AGE) formation and vascular endothelial cell growth factor (VEGF) overexpression. Twenty days after streptozotocin administration, diabetic rats were treated for 10 days with calcium dobesilate (100 mg/kg/day per os) or vehicle. Retinal albumin leakage, CML-AGE formation, and VEGF overexpression were evaluated by immunohistochemistry of frozen eye sections. Diabetic rats exhibited dramatic increases in: (i) retinal albumin leakage (31% of positive vessels vs. 0.2% in nondiabetic rats, P-4). Calcium dobesilate significantly reduced: (i) retinal albumin leakage (by 70%, P
| Original language | English |
|---|---|
| Pages (from-to) | 217-224 |
| Number of pages | 8 |
| Journal | European Journal of Pharmacology |
| Volume | 495 |
| Issue number | 2-3 |
| DOIs | |
| Publication status | Published - Jul 14 2004 |
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Keywords
- Advanced glycation end product
- Antioxidant
- Calcium dobesilate
- Diabetic retinopathy
- Vascular permeability
- VEGF (vascular endothelial cell growth factor)
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience
- Pharmacology
Cite this
Reduction of retinal albumin leakage by the antioxidant calcium dobesilate in streptozotocin-diabetic rats. / Rota, Rossella; Chiavaroli, Carlo; Garay, Ricardo P.; Hannaert, Patrick.
In: European Journal of Pharmacology, Vol. 495, No. 2-3, 14.07.2004, p. 217-224.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Reduction of retinal albumin leakage by the antioxidant calcium dobesilate in streptozotocin-diabetic rats
AU - Rota, Rossella
AU - Chiavaroli, Carlo
AU - Garay, Ricardo P.
AU - Hannaert, Patrick
PY - 2004/7/14
Y1 - 2004/7/14
N2 - Calcium dobesilate stabilizes blood-retinal barrier in patients with diabetic retinopathy and possesses antioxidant properties in the retinas of rats with streptozotocin-induced diabetes, exposed ex vivo to ischemia-reperfusion. Here we investigated the action of calcium dobesilate on retinal albumin leakage in streptozotocin-diabetic rats, together with relevant in vivo retinal antioxidant and permeability markers, i.e., carboxymethyl-lysine-advanced glycation end product (CML-AGE) formation and vascular endothelial cell growth factor (VEGF) overexpression. Twenty days after streptozotocin administration, diabetic rats were treated for 10 days with calcium dobesilate (100 mg/kg/day per os) or vehicle. Retinal albumin leakage, CML-AGE formation, and VEGF overexpression were evaluated by immunohistochemistry of frozen eye sections. Diabetic rats exhibited dramatic increases in: (i) retinal albumin leakage (31% of positive vessels vs. 0.2% in nondiabetic rats, P-4). Calcium dobesilate significantly reduced: (i) retinal albumin leakage (by 70%, P
AB - Calcium dobesilate stabilizes blood-retinal barrier in patients with diabetic retinopathy and possesses antioxidant properties in the retinas of rats with streptozotocin-induced diabetes, exposed ex vivo to ischemia-reperfusion. Here we investigated the action of calcium dobesilate on retinal albumin leakage in streptozotocin-diabetic rats, together with relevant in vivo retinal antioxidant and permeability markers, i.e., carboxymethyl-lysine-advanced glycation end product (CML-AGE) formation and vascular endothelial cell growth factor (VEGF) overexpression. Twenty days after streptozotocin administration, diabetic rats were treated for 10 days with calcium dobesilate (100 mg/kg/day per os) or vehicle. Retinal albumin leakage, CML-AGE formation, and VEGF overexpression were evaluated by immunohistochemistry of frozen eye sections. Diabetic rats exhibited dramatic increases in: (i) retinal albumin leakage (31% of positive vessels vs. 0.2% in nondiabetic rats, P-4). Calcium dobesilate significantly reduced: (i) retinal albumin leakage (by 70%, P
KW - Advanced glycation end product
KW - Antioxidant
KW - Calcium dobesilate
KW - Diabetic retinopathy
KW - Vascular permeability
KW - VEGF (vascular endothelial cell growth factor)
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UR - http://www.scopus.com/inward/citedby.url?scp=3042798298&partnerID=8YFLogxK
U2 - 10.1016/j.ejphar.2004.05.019
DO - 10.1016/j.ejphar.2004.05.019
M3 - Article
VL - 495
SP - 217
EP - 224
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 2-3
ER -