Abstract
Calcium dobesilate stabilizes blood-retinal barrier in patients with diabetic retinopathy and possesses antioxidant properties in the retinas of rats with streptozotocin-induced diabetes, exposed ex vivo to ischemia-reperfusion. Here we investigated the action of calcium dobesilate on retinal albumin leakage in streptozotocin-diabetic rats, together with relevant in vivo retinal antioxidant and permeability markers, i.e., carboxymethyl-lysine-advanced glycation end product (CML-AGE) formation and vascular endothelial cell growth factor (VEGF) overexpression. Twenty days after streptozotocin administration, diabetic rats were treated for 10 days with calcium dobesilate (100 mg/kg/day per os) or vehicle. Retinal albumin leakage, CML-AGE formation, and VEGF overexpression were evaluated by immunohistochemistry of frozen eye sections. Diabetic rats exhibited dramatic increases in: (i) retinal albumin leakage (31% of positive vessels vs. 0.2% in nondiabetic rats, P-4). Calcium dobesilate significantly reduced: (i) retinal albumin leakage (by 70%, P
| Original language | English |
|---|---|
| Pages (from-to) | 217-224 |
| Number of pages | 8 |
| Journal | European Journal of Pharmacology |
| Volume | 495 |
| Issue number | 2-3 |
| DOIs | |
| Publication status | Published - Jul 14 2004 |
Keywords
- Advanced glycation end product
- Antioxidant
- Calcium dobesilate
- Diabetic retinopathy
- Vascular permeability
- VEGF (vascular endothelial cell growth factor)
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience
- Pharmacology