Reduction of retinal albumin leakage by the antioxidant calcium dobesilate in streptozotocin-diabetic rats

Rossella Rota, Carlo Chiavaroli, Ricardo P. Garay, Patrick Hannaert

Research output: Contribution to journalArticlepeer-review

Abstract

Calcium dobesilate stabilizes blood-retinal barrier in patients with diabetic retinopathy and possesses antioxidant properties in the retinas of rats with streptozotocin-induced diabetes, exposed ex vivo to ischemia-reperfusion. Here we investigated the action of calcium dobesilate on retinal albumin leakage in streptozotocin-diabetic rats, together with relevant in vivo retinal antioxidant and permeability markers, i.e., carboxymethyl-lysine-advanced glycation end product (CML-AGE) formation and vascular endothelial cell growth factor (VEGF) overexpression. Twenty days after streptozotocin administration, diabetic rats were treated for 10 days with calcium dobesilate (100 mg/kg/day per os) or vehicle. Retinal albumin leakage, CML-AGE formation, and VEGF overexpression were evaluated by immunohistochemistry of frozen eye sections. Diabetic rats exhibited dramatic increases in: (i) retinal albumin leakage (31% of positive vessels vs. 0.2% in nondiabetic rats, P-4). Calcium dobesilate significantly reduced: (i) retinal albumin leakage (by 70%, P

Original languageEnglish
Pages (from-to)217-224
Number of pages8
JournalEuropean Journal of Pharmacology
Volume495
Issue number2-3
DOIs
Publication statusPublished - Jul 14 2004

Keywords

  • Advanced glycation end product
  • Antioxidant
  • Calcium dobesilate
  • Diabetic retinopathy
  • Vascular permeability
  • VEGF (vascular endothelial cell growth factor)

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

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