Reductive nitrosylation and peroxynitrite-mediated oxidation of heme-hemopexin

Paolo Ascenzi, Alessio Bocedi, Giovanni Antonini, Martino Bolognesi, Mauro Fasano

Research output: Contribution to journalArticle

Abstract

Hemopexin (HPX), which serves as a scavenger and transporter of toxic plasma heme, has been postulated to play a key role in the homeostasis of NO. In fact, HPX-heme(II) reversibly binds NO and facilitates NO scavenging by O 2. HPX-heme is formed by two four-bladed β-propeller domains. The heme is bound between the two β-propeller domains, residues His213 and His266 coordinate the heme iron atom. HPX-heme displays structural features of heme-proteins endowed with (pseudo-)enzymatic activities. In this study, the kinetics of rabbit HPX-heme(III) reductive nitrosylation and peroxynitrite-mediated oxidation of HPX-heme(II)-NO are reported. In the presence of excess NO, HPX-heme(III) is converted to HPX-heme(II)-NO by reductive nitrosylation. The second-order rate constant for HPX-heme(III) reductive nitrosylation is (1.3 ± 0.1) × 101 m -1·s-1, at pH 7.0 and 10.0 °C. NO binding to HPX-heme(III) is rate limiting. In the absence and presence of CO2 (1.2 × 10-3 m), excess peroxynitrite reacts with HPX-heme(II)-NO (2.6 × 10-6 m) leading to HPX-heme(III) and NO, via the transient HPX-heme(III)-NO species. Values of the second-order rate constant for HPX-heme(III)-NO formation are (8.6 ± 0.8) × 10 4 and (1.2 ± 0.2) × 106 m -1·s-1 in the absence and presence of CO 2, respectively, at pH 7.0 and 10.0°C. The CO2- independent value of the first-order rate constant for HPX-heme(III)-NO denitrosylation is (4.3 ± 0.4) × 10-1 s-1, at pH 7.0 and 10.0°C. HPX-heme(III)-NO denitrosylation is rate limiting. HPX-heme(II)-NO appears to act as an efficient scavenger of peroxynitrite and of strong oxidants and nitrating species following the reaction of peroxynitrite with CO2 (e.g. ONOOC(O)O-, CO3 -, and NO2).

Original languageEnglish
Pages (from-to)551-562
Number of pages12
JournalFEBS Journal
Volume274
Issue number2
DOIs
Publication statusPublished - Jan 2007

Keywords

  • Nitric oxide
  • Peroxynitrite
  • Peroxynitrite-mediated oxidation
  • Rabbit hemopexin
  • Reductive nitrosylation

ASJC Scopus subject areas

  • Biochemistry

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    Ascenzi, P., Bocedi, A., Antonini, G., Bolognesi, M., & Fasano, M. (2007). Reductive nitrosylation and peroxynitrite-mediated oxidation of heme-hemopexin. FEBS Journal, 274(2), 551-562. https://doi.org/10.1111/j.1742-4658.2006.05609.x