Reed-Sternberg cells and their cell microenvironment in Hodgkin's disease with reference to macrophage-histiocytes and interdigitating reticulum cells

A. Carbone, R. Manconi, A. Poletti, S. Sulfaro, A. Menin, U. Tirelli, P. G. Betta, R. Volpe

Research output: Contribution to journalArticle

Abstract

Fity-eight paraffin-embedded lymph node biopsies from patients with Hodgkin's disease (36 nodular sclerosis, 14 mixed cellularity, five lymphocyte depletion, and three lymphocyte predominance) were immunostained with a panel of monoclonal (anti-Leu-M1, antileukocyte common antigen) and polyclonal (to lysozyme, alpha1-antitrypsin, alpha1-antichymotrypsin, and S-100 protein) antibodies by using the avidin-biotin immunoperoxidase technique. Both the immunostaining features of the Reed-Sternberg (R-S) cells and their variants, and the numbers of immunostained accompanying cells morphologically corresponding to macrophage-histiocytes (M-H) and to interdigitating reticulum cells (IRC) were analyzed. Variable numbers of R-S cells and their variants were positive for Leu-M1 in 83% of the cases, for alpha1-antitrypsin in 40%, for alpha1-antichymotrypsin in 30%, and for leukocyte common antigen in 3.4%; they were constantly negative for lysozyme and S-100 protein. Whereas the average numbers of accompanying cells immunostained for Leu-M1 were very low, the numbers of S-100-positive IRC were relatively high in all the Hodgkin's subtypes. The average numbers of M-H were lower (P <0.1 for lysozyme; P <0.001 for alpha1-antichymotrypsin) in the nodular sclerosis than in the other pooled subtypes. In the nodular sclerosis subtype, however, R-S cells and their variants that stained positive for Leu-M1 appeared to express more frequently the lineage markers of M-H (alpha1-antitrypsin and/or alpha1-antichymotrypsin). These data appear to suggest that there is not an apparent qualitative correspondence between the immunostaining features of the cellular microenvironment composed of M-H and IRC and the features of the R-S cells.

Original languageEnglish
Pages (from-to)2662-2668
Number of pages7
JournalCancer
Volume60
Issue number11
Publication statusPublished - 1987

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Carbone, A., Manconi, R., Poletti, A., Sulfaro, S., Menin, A., Tirelli, U., Betta, P. G., & Volpe, R. (1987). Reed-Sternberg cells and their cell microenvironment in Hodgkin's disease with reference to macrophage-histiocytes and interdigitating reticulum cells. Cancer, 60(11), 2662-2668.