Reed-Sternberg cells and their cell microenvironment in Hodgkin's disease with reference to macrophage-histiocytes and interdigitating reticulum cells

A. Carbone, R. Manconi, A. Poletti, S. Sulfaro, A. Menin, U. Tirelli, P. G. Betta, R. Volpe

Research output: Contribution to journalArticle

Abstract

Fity-eight paraffin-embedded lymph node biopsies from patients with Hodgkin's disease (36 nodular sclerosis, 14 mixed cellularity, five lymphocyte depletion, and three lymphocyte predominance) were immunostained with a panel of monoclonal (anti-Leu-M1, antileukocyte common antigen) and polyclonal (to lysozyme, alpha1-antitrypsin, alpha1-antichymotrypsin, and S-100 protein) antibodies by using the avidin-biotin immunoperoxidase technique. Both the immunostaining features of the Reed-Sternberg (R-S) cells and their variants, and the numbers of immunostained accompanying cells morphologically corresponding to macrophage-histiocytes (M-H) and to interdigitating reticulum cells (IRC) were analyzed. Variable numbers of R-S cells and their variants were positive for Leu-M1 in 83% of the cases, for alpha1-antitrypsin in 40%, for alpha1-antichymotrypsin in 30%, and for leukocyte common antigen in 3.4%; they were constantly negative for lysozyme and S-100 protein. Whereas the average numbers of accompanying cells immunostained for Leu-M1 were very low, the numbers of S-100-positive IRC were relatively high in all the Hodgkin's subtypes. The average numbers of M-H were lower (P <0.1 for lysozyme; P <0.001 for alpha1-antichymotrypsin) in the nodular sclerosis than in the other pooled subtypes. In the nodular sclerosis subtype, however, R-S cells and their variants that stained positive for Leu-M1 appeared to express more frequently the lineage markers of M-H (alpha1-antitrypsin and/or alpha1-antichymotrypsin). These data appear to suggest that there is not an apparent qualitative correspondence between the immunostaining features of the cellular microenvironment composed of M-H and IRC and the features of the R-S cells.

Original languageEnglish
Pages (from-to)2662-2668
Number of pages7
JournalCancer
Volume60
Issue number11
Publication statusPublished - 1987

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Reed-Sternberg Cells
Cellular Microenvironment
Reticulum
Histiocytes
Hodgkin Disease
Dendritic Cells
Sclerosis
Muramidase
Macrophages
S100 Proteins
Lymphocyte Depletion
CD45 Antigens
Avidin
Biotin
Immunoenzyme Techniques
Paraffin
Cell Count
Lymph Nodes
Lymphocytes
Biopsy

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Carbone, A., Manconi, R., Poletti, A., Sulfaro, S., Menin, A., Tirelli, U., ... Volpe, R. (1987). Reed-Sternberg cells and their cell microenvironment in Hodgkin's disease with reference to macrophage-histiocytes and interdigitating reticulum cells. Cancer, 60(11), 2662-2668.

Reed-Sternberg cells and their cell microenvironment in Hodgkin's disease with reference to macrophage-histiocytes and interdigitating reticulum cells. / Carbone, A.; Manconi, R.; Poletti, A.; Sulfaro, S.; Menin, A.; Tirelli, U.; Betta, P. G.; Volpe, R.

In: Cancer, Vol. 60, No. 11, 1987, p. 2662-2668.

Research output: Contribution to journalArticle

Carbone, A, Manconi, R, Poletti, A, Sulfaro, S, Menin, A, Tirelli, U, Betta, PG & Volpe, R 1987, 'Reed-Sternberg cells and their cell microenvironment in Hodgkin's disease with reference to macrophage-histiocytes and interdigitating reticulum cells', Cancer, vol. 60, no. 11, pp. 2662-2668.
Carbone, A. ; Manconi, R. ; Poletti, A. ; Sulfaro, S. ; Menin, A. ; Tirelli, U. ; Betta, P. G. ; Volpe, R. / Reed-Sternberg cells and their cell microenvironment in Hodgkin's disease with reference to macrophage-histiocytes and interdigitating reticulum cells. In: Cancer. 1987 ; Vol. 60, No. 11. pp. 2662-2668.
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abstract = "Fity-eight paraffin-embedded lymph node biopsies from patients with Hodgkin's disease (36 nodular sclerosis, 14 mixed cellularity, five lymphocyte depletion, and three lymphocyte predominance) were immunostained with a panel of monoclonal (anti-Leu-M1, antileukocyte common antigen) and polyclonal (to lysozyme, alpha1-antitrypsin, alpha1-antichymotrypsin, and S-100 protein) antibodies by using the avidin-biotin immunoperoxidase technique. Both the immunostaining features of the Reed-Sternberg (R-S) cells and their variants, and the numbers of immunostained accompanying cells morphologically corresponding to macrophage-histiocytes (M-H) and to interdigitating reticulum cells (IRC) were analyzed. Variable numbers of R-S cells and their variants were positive for Leu-M1 in 83{\%} of the cases, for alpha1-antitrypsin in 40{\%}, for alpha1-antichymotrypsin in 30{\%}, and for leukocyte common antigen in 3.4{\%}; they were constantly negative for lysozyme and S-100 protein. Whereas the average numbers of accompanying cells immunostained for Leu-M1 were very low, the numbers of S-100-positive IRC were relatively high in all the Hodgkin's subtypes. The average numbers of M-H were lower (P <0.1 for lysozyme; P <0.001 for alpha1-antichymotrypsin) in the nodular sclerosis than in the other pooled subtypes. In the nodular sclerosis subtype, however, R-S cells and their variants that stained positive for Leu-M1 appeared to express more frequently the lineage markers of M-H (alpha1-antitrypsin and/or alpha1-antichymotrypsin). These data appear to suggest that there is not an apparent qualitative correspondence between the immunostaining features of the cellular microenvironment composed of M-H and IRC and the features of the R-S cells.",
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AU - Carbone, A.

AU - Manconi, R.

AU - Poletti, A.

AU - Sulfaro, S.

AU - Menin, A.

AU - Tirelli, U.

AU - Betta, P. G.

AU - Volpe, R.

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N2 - Fity-eight paraffin-embedded lymph node biopsies from patients with Hodgkin's disease (36 nodular sclerosis, 14 mixed cellularity, five lymphocyte depletion, and three lymphocyte predominance) were immunostained with a panel of monoclonal (anti-Leu-M1, antileukocyte common antigen) and polyclonal (to lysozyme, alpha1-antitrypsin, alpha1-antichymotrypsin, and S-100 protein) antibodies by using the avidin-biotin immunoperoxidase technique. Both the immunostaining features of the Reed-Sternberg (R-S) cells and their variants, and the numbers of immunostained accompanying cells morphologically corresponding to macrophage-histiocytes (M-H) and to interdigitating reticulum cells (IRC) were analyzed. Variable numbers of R-S cells and their variants were positive for Leu-M1 in 83% of the cases, for alpha1-antitrypsin in 40%, for alpha1-antichymotrypsin in 30%, and for leukocyte common antigen in 3.4%; they were constantly negative for lysozyme and S-100 protein. Whereas the average numbers of accompanying cells immunostained for Leu-M1 were very low, the numbers of S-100-positive IRC were relatively high in all the Hodgkin's subtypes. The average numbers of M-H were lower (P <0.1 for lysozyme; P <0.001 for alpha1-antichymotrypsin) in the nodular sclerosis than in the other pooled subtypes. In the nodular sclerosis subtype, however, R-S cells and their variants that stained positive for Leu-M1 appeared to express more frequently the lineage markers of M-H (alpha1-antitrypsin and/or alpha1-antichymotrypsin). These data appear to suggest that there is not an apparent qualitative correspondence between the immunostaining features of the cellular microenvironment composed of M-H and IRC and the features of the R-S cells.

AB - Fity-eight paraffin-embedded lymph node biopsies from patients with Hodgkin's disease (36 nodular sclerosis, 14 mixed cellularity, five lymphocyte depletion, and three lymphocyte predominance) were immunostained with a panel of monoclonal (anti-Leu-M1, antileukocyte common antigen) and polyclonal (to lysozyme, alpha1-antitrypsin, alpha1-antichymotrypsin, and S-100 protein) antibodies by using the avidin-biotin immunoperoxidase technique. Both the immunostaining features of the Reed-Sternberg (R-S) cells and their variants, and the numbers of immunostained accompanying cells morphologically corresponding to macrophage-histiocytes (M-H) and to interdigitating reticulum cells (IRC) were analyzed. Variable numbers of R-S cells and their variants were positive for Leu-M1 in 83% of the cases, for alpha1-antitrypsin in 40%, for alpha1-antichymotrypsin in 30%, and for leukocyte common antigen in 3.4%; they were constantly negative for lysozyme and S-100 protein. Whereas the average numbers of accompanying cells immunostained for Leu-M1 were very low, the numbers of S-100-positive IRC were relatively high in all the Hodgkin's subtypes. The average numbers of M-H were lower (P <0.1 for lysozyme; P <0.001 for alpha1-antichymotrypsin) in the nodular sclerosis than in the other pooled subtypes. In the nodular sclerosis subtype, however, R-S cells and their variants that stained positive for Leu-M1 appeared to express more frequently the lineage markers of M-H (alpha1-antitrypsin and/or alpha1-antichymotrypsin). These data appear to suggest that there is not an apparent qualitative correspondence between the immunostaining features of the cellular microenvironment composed of M-H and IRC and the features of the R-S cells.

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