Reevaluation of the evidence that an antibody to the insulin receptor is insulinmimetic without activating the protein tyrosine kinase activity of the receptor

R. Gherzi, D. S. Russell, S. I. Taylor, O. M. Rosen

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The immunoglobulin fraction of a polyclonal anti-insulin receptor antibody (B-10) derived from a patient with severe insulin resistance and acanthosis nigricans was tested for its ability to activate the protein kinase activity of the insulin receptor and to mimic insulin action in Chinese hamster ovary cells expressing either wild type or kinase-deficient human insulin receptors. This antiserum had previously been reported to be insulinmimetic without activating the insulin receptor protein tyrosine kinase. Antibody B-10 bound to both wild type and mutant human insulin receptors, but it induced receptor down-regulation and stimulated hexose transport and thymidine incorporation into DNA only in cells expressing the wild type receptor. Furthermore, this antibody activated the kinase activity of the wild type insulin receptor in intact cells and in vitro. It is likely, therefore, that the biological activities of antibody B-10, like those of insulin, depend upon the protein tyrosine kinase activity of the insulin receptor.

Original languageEnglish
Pages (from-to)16900-16905
Number of pages6
JournalJournal of Biological Chemistry
Volume262
Issue number35
Publication statusPublished - 1987

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Insulin Receptor
Receptor Protein-Tyrosine Kinases
Protein-Tyrosine Kinases
Antibodies
Insulin
Phosphotransferases
Cells
Acanthosis Nigricans
Insulin Antibodies
Hexoses
Cricetulus
Bioactivity
Thymidine
Protein Kinases
Insulin Resistance
Immunoglobulins
Immune Sera
Ovary
Down-Regulation
DNA

ASJC Scopus subject areas

  • Biochemistry

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Reevaluation of the evidence that an antibody to the insulin receptor is insulinmimetic without activating the protein tyrosine kinase activity of the receptor. / Gherzi, R.; Russell, D. S.; Taylor, S. I.; Rosen, O. M.

In: Journal of Biological Chemistry, Vol. 262, No. 35, 1987, p. 16900-16905.

Research output: Contribution to journalArticle

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