Reference profile correlation reveals estrogen-like trancriptional activity of curcumin

Beatrice E. Bachmeier, Valentina Mirisola, Francesco Romeo, Luca Generoso, Alessia Esposito, Raffaella Dell'Eva, Fabiola Blengio, Peter H. Killian, Adriana Albini, Ulrich Pfeffer

Research output: Contribution to journalArticle

Abstract

Background: Several secondary metabolites from herbal nutrient products act as weak estrogens (phytoestrogens), competing with endogenous estrogen for binding to the estrogen receptors and inhibiting steroid converting enzymes. However, it is still unclear whether these compounds elicit estrogen dependent transcription of genes at physiological concentrations. Methods: We compare the effects of physiological concentrations (100 nM) of the two phytoestrogens Enterolactone and Quercetin and the suspected phytoestrogen Curcumin on gene expression in the breast cancer cell line MCF7 with the effects elicited by 17-β-estradiol (E2). Results: All three phytocompounds have weak effects on gene transcription; most of the E2 genes respond to the phytoestrogens in the same direction though to a much lesser extent and in the order Curcumin > Quercetin > Enterolactone. Gene regulation induced by these compounds was low for genes strongly induced by E2 and similar to the latter for genes only weakly regulated by the classic estrogen. Of interest with regard to the treatment of menopausal symptoms, the survival factor Birc5/survivin and the oncogene MYBL1 are strongly induced by E2 but only marginally by phytoestrogens. Conclusion: This approach demonstrates estrogenic effects of putative phytoestrogens at physiological concentrations and shows, for the first time, estrogenic effects of Curcumin.

Original languageEnglish
Pages (from-to)471-482
Number of pages12
JournalCellular Physiology and Biochemistry
Volume26
Issue number3
DOIs
Publication statusPublished - 2010

Keywords

  • Breast cancer
  • Chemoprevention
  • Gene expression profiling
  • Phytoestrogen
  • Reference profile correlation

ASJC Scopus subject areas

  • Physiology

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    Bachmeier, B. E., Mirisola, V., Romeo, F., Generoso, L., Esposito, A., Dell'Eva, R., Blengio, F., Killian, P. H., Albini, A., & Pfeffer, U. (2010). Reference profile correlation reveals estrogen-like trancriptional activity of curcumin. Cellular Physiology and Biochemistry, 26(3), 471-482. https://doi.org/10.1159/000320570