Reference standardization and triglyceride interference of a new homogeneous HDL-cholesterol assay compared with a former chemical precipitation assay

Christa Cobbaert, Louwerens Zwang, Ferruccio Ceriotti, Annalisa Modenese, Peter Cremer, Wolfgang Herrmann, Gerhard Hoss, Jochen Jarausch, Regina Türk, Winfried März, Matthias Nauck

Research output: Contribution to journalArticlepeer-review

Abstract

A homogeneous HDL-c assay (HDL-H), which uses polyethylene glycol- modified enzymes and sulfated α-cyclodextrin, was assessed for precision, accuracy, and cholesterol and triglyceride interference. In addition, its analytical performance was compared with that of a phosphotungstic acid (PTA)/MgCl2 precipitation method (HDL-P). Within-run CVs were ≤1.87%; total CVs were ≤3.08%. Accuracy was evaluated in fresh normotriglyceridemic sera using the Designated Comparison Method (HDL-H = 1.037 Designated Comparison Method + 4 mg/L; n = 63) and in moderately hypertriglyceridemic sera by using the Reference Method (HDL-H = 1.068 Reference Method - 17 mg/L; n = 41). Mean biases were 4.5% and 2.2%, respectively. In hypertriglyceridemic sera (n = 85), HDL-H concentrations were increasingly positively biased with increasing triglyceride concentrations. The method comparison between HDL-H and HDL-P yielded the following equation: HDL-H = 1.037 HDL-P + 15 mg/L; n = 478. We conclude that HDL-H amply meets the 1998 NCEP recommendations for total error; its precision is superior compared with that of HDL-P, and its average bias remains below ±5% as long as triglyceride concentrations are ≤10 g/L and in case of moderate hypercholesterolemia.

Original languageEnglish
Pages (from-to)779-789
Number of pages11
JournalClinical Chemistry
Volume44
Issue number4
Publication statusPublished - 1998

ASJC Scopus subject areas

  • Clinical Biochemistry

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