Reference typing report for complement component C3

C. Rittner, D. Bellavia, G. Geserick, A. Frank, B. Stradmann-Bellinghausen

Research output: Contribution to journalArticle

Abstract

A comparison of five C3 variant samples has been performed by conventional high-voltage gel electrophoresis in three laboratories (Palermo, Berlin and Mainz). Local designation was shown within SD = ± 0.75 mm migration distance in the Mainz laboratory. Methodological modifications by laboratories were not accounted for (cooling temperature, relative mobilities between runs). In parallel, all reference samples were also sequenced after exon-specific amplification. As a result, two variants with identical final designations and two variants with different mobilities were shown to conform at the molecular basis exhibiting an amino acid exchange that causes the corresponding change in relative mobility as compared to normal C3F and C3S.

Original languageEnglish
Pages (from-to)264-267
Number of pages4
JournalExperimental and Clinical Immunogenetics
Volume15
Issue number4
Publication statusPublished - 1998

Keywords

  • C3 polymorphism
  • High-voltage gel electrophoresis
  • Molecular basis of variants

ASJC Scopus subject areas

  • Immunology
  • Genetics(clinical)
  • Genetics

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    Rittner, C., Bellavia, D., Geserick, G., Frank, A., & Stradmann-Bellinghausen, B. (1998). Reference typing report for complement component C3. Experimental and Clinical Immunogenetics, 15(4), 264-267.