Abstract
We induced brief secondarily generalized seizures of limbic origin in Sprague-Dawley rats by bicuculline microinfusion into the anterior piriform cortex. After 1 h or 5 days we performed comet assay, a sensitive marker for DNA damage, within entorhinal cortex, hippocampus (limbic areas recruited by seizure spreading) and striatum (which is not recruited). DNA damage occurred selectively in the ipsilateral entorhinal cortex and hippocampus at 1 h, but not at 5 days. These data shed new light on molecular genetics as a marker during limbic seizures, the most common in epileptic patients.
| Original language | English |
|---|---|
| Pages (from-to) | 1399-1403 |
| Number of pages | 5 |
| Journal | Journal of Neural Transmission |
| Volume | 121 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- Comet assay
- DNA integrity
- Entorhinal cortex
- Epilepsy
- Hippocampus
- Limbic seizures
ASJC Scopus subject areas
- Biological Psychiatry
- Neurology
- Clinical Neurology
- Psychiatry and Mental health
- Medicine(all)
Cite this
Region-specific DNA alterations in focally induced seizures. / Cantafora, Emanuela; Giorgi, Filippo Sean; Frenzilli, Giada; Scarcelli, Vittoria; Busceti, Carla L.; Nigro, Marco; Bernardeschi, Margherita; Fornai, Francesco.
In: Journal of Neural Transmission, Vol. 121, No. 11, 2014, p. 1399-1403.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Region-specific DNA alterations in focally induced seizures
AU - Cantafora, Emanuela
AU - Giorgi, Filippo Sean
AU - Frenzilli, Giada
AU - Scarcelli, Vittoria
AU - Busceti, Carla L.
AU - Nigro, Marco
AU - Bernardeschi, Margherita
AU - Fornai, Francesco
PY - 2014
Y1 - 2014
N2 - We induced brief secondarily generalized seizures of limbic origin in Sprague-Dawley rats by bicuculline microinfusion into the anterior piriform cortex. After 1 h or 5 days we performed comet assay, a sensitive marker for DNA damage, within entorhinal cortex, hippocampus (limbic areas recruited by seizure spreading) and striatum (which is not recruited). DNA damage occurred selectively in the ipsilateral entorhinal cortex and hippocampus at 1 h, but not at 5 days. These data shed new light on molecular genetics as a marker during limbic seizures, the most common in epileptic patients.
AB - We induced brief secondarily generalized seizures of limbic origin in Sprague-Dawley rats by bicuculline microinfusion into the anterior piriform cortex. After 1 h or 5 days we performed comet assay, a sensitive marker for DNA damage, within entorhinal cortex, hippocampus (limbic areas recruited by seizure spreading) and striatum (which is not recruited). DNA damage occurred selectively in the ipsilateral entorhinal cortex and hippocampus at 1 h, but not at 5 days. These data shed new light on molecular genetics as a marker during limbic seizures, the most common in epileptic patients.
KW - Comet assay
KW - DNA integrity
KW - Entorhinal cortex
KW - Epilepsy
KW - Hippocampus
KW - Limbic seizures
UR - http://www.scopus.com/inward/record.url?scp=84925708908&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84925708908&partnerID=8YFLogxK
U2 - 10.1007/s00702-014-1211-5
DO - 10.1007/s00702-014-1211-5
M3 - Article
C2 - 24748548
AN - SCOPUS:84925708908
VL - 121
SP - 1399
EP - 1403
JO - Journal of Neural Transmission
JF - Journal of Neural Transmission
SN - 0300-9564
IS - 11
ER -