Region-specific DNA alterations in focally induced seizures

Emanuela Cantafora, Filippo Sean Giorgi, Giada Frenzilli, Vittoria Scarcelli, Carla L. Busceti, Marco Nigro, Margherita Bernardeschi, Francesco Fornai

Research output: Contribution to journalArticle

Abstract

We induced brief secondarily generalized seizures of limbic origin in Sprague-Dawley rats by bicuculline microinfusion into the anterior piriform cortex. After 1 h or 5 days we performed comet assay, a sensitive marker for DNA damage, within entorhinal cortex, hippocampus (limbic areas recruited by seizure spreading) and striatum (which is not recruited). DNA damage occurred selectively in the ipsilateral entorhinal cortex and hippocampus at 1 h, but not at 5 days. These data shed new light on molecular genetics as a marker during limbic seizures, the most common in epileptic patients.

Original languageEnglish
Pages (from-to)1399-1403
Number of pages5
JournalJournal of Neural Transmission
Volume121
Issue number11
DOIs
Publication statusPublished - 2014

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Keywords

  • Comet assay
  • DNA integrity
  • Entorhinal cortex
  • Epilepsy
  • Hippocampus
  • Limbic seizures

ASJC Scopus subject areas

  • Biological Psychiatry
  • Neurology
  • Clinical Neurology
  • Psychiatry and Mental health
  • Medicine(all)

Cite this

Cantafora, E., Giorgi, F. S., Frenzilli, G., Scarcelli, V., Busceti, C. L., Nigro, M., Bernardeschi, M., & Fornai, F. (2014). Region-specific DNA alterations in focally induced seizures. Journal of Neural Transmission, 121(11), 1399-1403. https://doi.org/10.1007/s00702-014-1211-5