Region-specific DNA alterations in focally induced seizures

Emanuela Cantafora; Filippo Sean Giorgi; Giada Frenzilli; Vittoria Scarcelli; Carla L. Busceti; Marco Nigro; Margherita Bernardeschi; Francesco Fornai

doi:10.1007/s00702-014-1211-5

Region-specific DNA alterations in focally induced seizures

Emanuela Cantafora, Filippo Sean Giorgi, Giada Frenzilli, Vittoria Scarcelli, Carla L. Busceti, Marco Nigro, Margherita Bernardeschi, Francesco Fornai

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Abstract

We induced brief secondarily generalized seizures of limbic origin in Sprague-Dawley rats by bicuculline microinfusion into the anterior piriform cortex. After 1 h or 5 days we performed comet assay, a sensitive marker for DNA damage, within entorhinal cortex, hippocampus (limbic areas recruited by seizure spreading) and striatum (which is not recruited). DNA damage occurred selectively in the ipsilateral entorhinal cortex and hippocampus at 1 h, but not at 5 days. These data shed new light on molecular genetics as a marker during limbic seizures, the most common in epileptic patients.

Original language	English
Pages (from-to)	1399-1403
Number of pages	5
Journal	Journal of Neural Transmission
Volume	121
Issue number	11
DOIs	https://doi.org/10.1007/s00702-014-1211-5
Publication status	Published - 2014

Keywords

Comet assay
DNA integrity
Entorhinal cortex
Epilepsy
Hippocampus
Limbic seizures

ASJC Scopus subject areas

Biological Psychiatry
Neurology
Clinical Neurology
Psychiatry and Mental health
Medicine(all)

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10.1007/s00702-014-1211-5

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AU - Cantafora, Emanuela

AU - Giorgi, Filippo Sean

AU - Frenzilli, Giada

AU - Scarcelli, Vittoria

AU - Busceti, Carla L.

AU - Nigro, Marco

AU - Bernardeschi, Margherita

AU - Fornai, Francesco

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AB - We induced brief secondarily generalized seizures of limbic origin in Sprague-Dawley rats by bicuculline microinfusion into the anterior piriform cortex. After 1 h or 5 days we performed comet assay, a sensitive marker for DNA damage, within entorhinal cortex, hippocampus (limbic areas recruited by seizure spreading) and striatum (which is not recruited). DNA damage occurred selectively in the ipsilateral entorhinal cortex and hippocampus at 1 h, but not at 5 days. These data shed new light on molecular genetics as a marker during limbic seizures, the most common in epileptic patients.

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KW - Hippocampus

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Region-specific DNA alterations in focally induced seizures

Abstract

Keywords

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