Regional development of carbachol-, glutamate-, norepinephrine-, and serotonin-stimulated phosphoinositide metabolism in rat brain

Walter Balduini, Stefano M. Candura, Lucio G. Costa

Research output: Contribution to journalArticle

Abstract

Phosphoinositide metabolism stimulated by activation of cholinergic muscarinic, glutamatergic, α-adrenergic and serotoninergic receptors was measured in brain regions of the developing rats. Accumulation of [3H]inositol phosphates ([3H]InsPs) in [3H]inositol-prelabeled slices from cerebral cortex, hippocampus, brainstem and cerebellum was measured as an index of phosphoinositide metabolism. Large age-, neurotransmitter receptor-, and brain region-dependent differences were found. Carbachol-stimulated [3H]InsPs accumulation peaked on postnatal day 7 in cerebral cortex and hippocampus while in cerebellum and brainstem the effect of muscarinic stimulation was maximal at birth and then declined to adulthood. The effect of glutamate also showed a peak on day 7 in hippocampus and brainstem and a developmentally related decrease in cerebral cortex. In the cerebellum, on the other hand, the response to glutamate remained sustained through adulthood. Stimulation of phosphoinositide metabolism by norepinephrine increased with age in hippocampus and cerebral cortex, but decreased in the cerebellum, while the effect of serotonin did not change significantly with age except in cerebellum. These changes in receptor-stimulated phosphoinositide metabolism do not parallel, for the most part, the ontogeny of receptor recognition sites. Activation of the phosphoinositide metabolism pathway leads to an increase in intracellular calcium levels and to stimulation of protein kinase C, which are believed to play significant roles in cellular proliferation and differentiation. Thus, the differential ability of neurotransmitters to stimulate phosphoinositide hydrolysis might play a role in the development of brain regions.

Original languageEnglish
Pages (from-to)115-120
Number of pages6
JournalDevelopmental Brain Research
Volume62
Issue number1
DOIs
Publication statusPublished - Sep 19 1991

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Carbachol
Phosphatidylinositols
Cerebellum
Glutamic Acid
Serotonin
Norepinephrine
Cerebral Cortex
Hippocampus
Cholinergic Agents
Brain
Brain Stem
Neurotransmitter Receptor
Inositol Phosphates
Inositol
Adrenergic Receptors
Protein Kinase C
Neurotransmitter Agents
Hydrolysis
Cell Proliferation
Parturition

Keywords

  • Carbachol
  • Glutamate
  • Noradrenaline
  • Phosphoinositide metabolism
  • Serotonin

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Regional development of carbachol-, glutamate-, norepinephrine-, and serotonin-stimulated phosphoinositide metabolism in rat brain. / Balduini, Walter; Candura, Stefano M.; Costa, Lucio G.

In: Developmental Brain Research, Vol. 62, No. 1, 19.09.1991, p. 115-120.

Research output: Contribution to journalArticle

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